Transactions of the Korean hydrogen and new energy society (한국수소및신에너지학회논문집)

The Korean Hydrogen and New Energy Society (한국수소및신에너지학회)
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Optimization of Hydrogen Production Using the Mixed Microflora Isolated from Sewage Sludge

하수슬러지로부터 분리된 혐기세균에 의한 수소생산 최적화

  • Oh, You-Kwan (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Kim, Mi-Sun (Bioenergy Research Center, Korea Institute of Energy Research)
  • 오유관 (한국에너지기술연구원 바이오에너지연구센터) ;
  • 김미선 (한국에너지기술연구원 바이오에너지연구센터)
  • Published : 2008.02.29
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Abstract

Fermentative $H_2$ production was studied using microbial consortia isolated from heat-treated ($90{\circ}C$, 20 min) sewage sludge. Important parameters investigated were carbon(C) and nitrogen(N)-sources, C/N ratio, phosphate concentration, pH and temperature during anaerobic cultivation in serum bottles. Starch, ribose, sucrose and glucose were good C-sources for the culture growth and $H_2$ production. Yeast extract was better N-source than $(NH_4)_2SO_4$ or peptone when individually added to the synthetic media, however the combination of above three N-sources exhibited the additional effect for cell growth and $H_2$ evolution. Addition of 100 mM phosphate as a buffering agent prevented the rapid pH drop during the cultivation. The optimum initial pH for the cell growth was at 7.0, whereas $H_2$ production was observed at pH 5.5. Optimum temperature for the cell growth and $H_2$ production was $37{\circ}C$. Initial C/N ratio of 1.22 in the media using glucose and yeast extract as the C- and N-sources, respectively, showed the $H_2$ yield 1.0 mol $H_2$/mol glucose.

Keywords

References

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