Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Agrobactrium tumefaciens-Mediated Transformation of Monascus ruber

  • Yang, Yun-Jung (Department of Foods and Nutrition, Kookmin University) ;
  • Lee, In-Hyung (Department of Foods and Nutrition, Kookmin University)
  • Published : 2008.04.30
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Abstract

Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to Monascus ruber. The optimum cocultivation time was 84 h with an efficiency of 900 to 1,000 transformants when $1{\times}10^6$ spores were used with the same volume of bacteria. The stability of transform ants was over 98% after five generations. When M. ruber was transformed with A. tumefaciens YL-63 containing the green fluorescent protein gene (egfp), the green fluorescent signal was observed throughout hyphae, confirming expression of the gene. This efficient transformation and expression system of M. ruber by ATMT will facilitate the study of this fungus at a molecular genetic level.

Keywords

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