Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Cyclodextrins in Ultrafiltration Membrane Reactor Containing Cyclodextrin Glycosyltransferase from Bacillus macerans

  • Son, Young-Jin (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University) ;
  • Rha, Chan-Su (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University) ;
  • Park, Yong-Cheol (Center for Agricultural Biomaterials, Seoul National University) ;
  • Shin, So-Yeon (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lee, Yoon-Seung (Department of Agricultural Biotechnology, Seoul National University) ;
  • Seo, Jin-Ho (Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University)
  • Published : 2008.04.30
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Abstract

An enzyme reactor installed with ultrafiltration membrane was developed to produce ${\alpha}-,\;{\beta}-$, and ${\gamma}$-cyclodextrins (CDs) from soluble starch by Bacillus macerans cyclodextrin glycosyltransferase (CGTase) tagged with 10 lysines at its C-terminus (CGTKIOase). Ultrafiltration membrane YM10 with 10,000 of molecular cutoff was chosen for membrane modification and CD production. A repeated-batch type of the enzyme reaction with free CGTK10ase resulted in a ${\alpha}$-CD yield of 24.0 (${\pm}1.5$)% and a productivity of 4.68 (${\pm}0.88$) g/l-h, which were 7 times higher that those for CGTK10ase immobilized on modified YM10 membrane. Addition of 1-nonanol increased CD yields by 30% relative to the control, which might be due to prevention of the reversible hydrolysis of CDs.

Keywords

References

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