Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Oxygen and Shear Stress on Molecular Weight of Hyaluronic Acid Produced by Streptococcus zooepidemicus

  • Duan, Xu-Jie (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Yang, Li (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Zhang, Xu (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology) ;
  • Tan, Wen-Song (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology)
  • Published : 2008.04.30
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Abstract

Dissolved oxygen (DO) and shear stress have pronounced effects on hyaluronic acid (HA) production, yet various views persist about their effects on the molecular weight of HA. Accordingly, this study investigated the effects of DO and shear stress during HA fermentation. The results showed that both cell growth and HA synthesis were suppressed under anaerobic conditions, and the HA molecular mass was only $(1.22{\pm}0.02){\times}10^6 Da$. Under aerobic conditions, although the DO level produced no change in the biomass or HA yield, a high DO level favored the HA molecular mass, which reached a maximum value of $(2.19{\pm}0.05){\times}10^6 Da$ at 50% DO. Furthermore, a high shear stress delayed the rate of HA synthesis and decreased the HA molecular weight, yet had no clear effect on the HA yield. Therefore, a high DO concentration and mild shear environment would appear to be essential to enhance the HA molecular weight.

Keywords

References

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