Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Uncultivable Anaerobic Thermophiles of the Family Clostridiaceae Requiring Growth-Supporting Factors

  • Kim, Joong-Jae (Department of Biological Sciences, Graduate School of Science, Osaka University) ;
  • Kim, Hee-Na (Department of Biological Sciences, Graduate School of Science, Osaka University) ;
  • Masui, Ryoji (Department of Biological Sciences, Graduate School of Science, Osaka University) ;
  • Kuramitsu, Seiki (Department of Biological Sciences, Graduate School of Science, Osaka University) ;
  • Seo, Jin-Ho (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Kwang (Department of Biological Sciences, Graduate School of Science, Osaka University) ;
  • Sung, Moon-Hee (Department of Bio & Nanochemistry, Kookmin University, BioLeaders Corporation)
  • Published : 2008.04.30
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Abstract

Novel groups of uncultivable anaerobic thermophiles were isolated from compost by enrichment cultivation in medium with a cell-free extract of Geobacillus toebii. The cell-free extract of G. toebii provided the medium with growth-supporting factors (GSF) needed to cultivate the previously uncultured microorganisms. Twenty-nine GSF-requiring candidates were successfully cultivated, and 16 isolated novel bacterial strains were classified into three different groups of uncultivable bacteria. The similarity among these 16 isolates and a phylogenetic analysis using 16S rRNA gene sequences revealed that these GSF-requiring strains represented novel groups within the family Clostridiaceae.

Keywords

References

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