난소절제 쥐에서 카페인 첨가식이가 골밀도 및 골함량에 미치는 영향

Effects of Caffeine on Bone Mineral Density and Bone Mineral Content in Ovariectomized Rats

  • 최미자 (계명대학교 식품영양학과) ;
  • 이주영 (계명대학교 식품영양학과)
  • Choi, Mi-Ja (Department of Food and Nutrition, Keimyung University) ;
  • Lee, Joo-Young (Department of Food and Nutrition, Keimyung University)
  • 발행 : 2008.04.30

초록

본 연구는 0.03%의 카페인 함유 식이를 폐경모델인 난소절제쥐에서 6주간 섭취시켜 골밀도와 골무기질함량에 미치는 영향을 아래와 같이 요약하였다. 1) 체중증가량은 난소절제군이 Sham군에 비해 유의적으로 높았으며 각 군내에서 카페인 섭취에 따른 차이는 없었다. 2) 혈 중 칼슘 농도는 난소절제군내에서 카페인군이 대조군보다 유의적으로 낮게 나타났다. 3) 혈 중 ALP는 Sham군과 난소절제군 모두에서 카페인 군이 대조군보다 높은 경향을 나타내었다. 혈중 Osteocalcin은 Sham 군과 난소절제군, 그리고 각 군내에서 카페인 섭취여부에 따라 유의적인 차이를 나타내지 않았다. 4) 요 중 칼슘 및 인의 농도, 요 중 Deoxypyridinoline(DPD)와 crosslinks value는 Sham 군과 난소절제군, 그리고 각 군내에서 카페인 섭취여부에 따라 유의적인 차이를 나타내지 않았다. 5) 척추골밀도는 Sham군에 비해 난소절제군이 유의적으로 낮았고, 난소절제군내에서 카페인 첨가군과 대조군간에 차이가 없었다. 6) 대퇴골밀도와 대퇴 골무기질 함량은 Sham군과 난소 절제군 간의 차이는 없었고, 각 군내에서 식이에 따른 차이도 없었다. 따라서 카페인 0.03% caffeine 섭취는 난소절제쥐에서 6주간 섭취 시킨 경우 척추와 대퇴골밀도에 부정적 영향을 미치지 않는 것으로 나타났다.

The purpose of this study was to examine the effects of dietary caffeine supplementation on bone mineral density and bone mineral content in ovariectomized rats. Twenty eight female Sprague-Dawley rats (body weight $210\;{\pm}\;5\;g$) were divided into two groups, ovariectomy (OVX) and Sham groups, which were each randomly divided into two subgroups that were fed control and control supplemented with caffeine diets (caffeine 0.03% diets). All rats were fed on experimental diet and deionized water ad libitum for 6 weeks. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin) in spine and femur. Serum alkaline phosphatase activity (ALP) and osteocalcin and urinary DPD crosslinks value were measured as markers of bone formation and resorption. The results of this study indicate that body weight gain and food intake were higher in OVX groups than in Sham groups regardless of diets. There were no differences weight gain between the control and caffeine groups in both OVX and Sham groups. Within the OVX groups, serum Ca concentration was lower in rats fed caffeine than in rats fed the control diet. Serum ALP, osteocalcin, urinary Ca, and phosphate were not different in each group. Spine BMD, spine BMD/weight, and spine BMC/weight, femur BMD/weight and femur BMC/weight of ovariectomy groups were significantly lower than Sham groups. Within the OVX group, there were no differences in spine BMD and BMC and femur BMD and BMC. These results indicate that no significant differences in spine and femur BMD were found due to 0.03% caffeine intakes in diet in OVX rats for 6 weeks. No negative effect of caffeine in 0.03% diet on bone mineral density were found in the present study. Further investigation of the relation between caffeine and bone mineral density are warranted. (KoreanJNutr2008; 41(3): 2l6~223)

키워드

참고문헌

  1. Korea National Statistical Office, Life Expectancy, Seoul; 2005
  2. Lee HJ, Lee HO. A study on the bone mineral density and related factors in korean postmenopausal women. Korean J Nutr 1999; 32(2): 197-203
  3. Lee YS. Micronutrients and osteoporosis. Korean J Osteoprosis 2005; 3: 206-230
  4. Bukowiecki LJ, Lupien J, Folla N, Jahjah L. Effect of sucrose, caffeine, and cola beverages on obesity, cold resistance, and adipose tissue cellularity. Am J Physiol 1983; 244: R500-R507
  5. Yoon MH, Lee MJ, Hwang SI, Moon SK, Kim JK, Jeong LH, Yim JR. A evaluation of caffeine contents in commercial foods. J Fd Hyg Safety 2001; 16(4): 295-299
  6. Lee HW. A study on caffeine containing foods and the effect of caffeine in humans. Kor J Culinary Res 2000; 6(3): 343-355
  7. Hernandez-Avila M, Colditz GA, Stamter MJ, Rosner B, SpeiZer FE, Willett WC: Caffeine, moderate alcohol intake, and the risk of the hip and forearm in middle-aged women. Am J Clin Nutr 1991; 54: 157-163 https://doi.org/10.1093/ajcn/54.1.157
  8. Lee JS, Hong HO, Yu CH. A study on the effect of caffeine intake on calcium and phosphorus metabolism in ovariectomized rats. Korean J Nutr 1996; 29(9): 950-957
  9. Choi MK, Lee YS, Sung CJ. Effects of caffeine intake on calcium utilization in rats of different age and sex. Korean J Nutr 1997; 30(8): 911-919
  10. Massey KL, Whiting SJ. Caffeine, urinary calcium, calcium metabolism and bone. J Nutr 1993; 123(9): 1161-1164
  11. Lloyd T, Rollings N, Douglas FE, Keselhorst, VM Chinchilli. Dietary caffeine intake and bone status of postmenopausal women. Am J Clin Nutr 1997; 65: 1826-1830 https://doi.org/10.1093/ajcn/65.6.1826
  12. Harris SS, Dawson-Hughes B. Caffeine and bone loss in healthy postmenopausal women. Am J Clin Nutr 1994; 60: 573-578 https://doi.org/10.1093/ajcn/60.4.573
  13. Wink Cs, Rossowska MJ, Nakamoto T. Effects of caffeine on bone cells and bone development in fast-growing rats. Pub Med 1996; 246(1): 30-39
  14. Ohta M, Check G, Ide K, Cheuk S, Wink CS, Falster AU, Simmons WB, Nakamoto T. Effects of caffeine on the bones of aged, ovariectomized rats. Ann Nutr Metab 1999; 43: 52-59 https://doi.org/10.1159/000012767
  15. Sakamoto W. Effect of coffee consumption on bone metabolism. Bone 2001; 28(3): 332-336 https://doi.org/10.1016/S8756-3282(00)00444-0
  16. Wronski TJ, Cinton M, Doherty AL, Donn LM. Estrgen treatment prevents osteopenia and depresses bone turnover in ovariectomized rats. Endocrinol 1988; 123: 681-688 https://doi.org/10.1210/endo-123-2-681
  17. Williams JP, Jordan SE, Barnes S, Blair HC. Tyrosine Kinase inhibitor effects on avian osteoclastic acid transport. Am J Clin Nutr 1998(6s); 1369-1374
  18. Ohta M, Cheuk G, Thomos KA, Kamagata-Kiyouraa Y, Winkc CS, Yazdania M, Falsterd AU, Simmonsd WB, Nakamotoa T. Effects of caffeine on bones of aged, ovariectomized rats. Ann Nutr Metab 1999; 43: 52-59 https://doi.org/10.1159/000012767
  19. Nakamoto T, Shaye R. Effects of caffeine on growth of mandible and long bone in protein-energy malnourished newborn rats. Proc Soc Exp Biol Med 1984; 177: 56-61
  20. Cheung WT, Lee CM, Ng TB. Potentiation of the antilipolytic effect of 2-chloroadenosine after chronic caffeine treatment. Pharmacol 1988; 36: 331-339 https://doi.org/10.1159/000138403
  21. Chung LW, Gleave ME, Hsi JT, Hong SJ, Zhau HE. Reciprocal mesenchymal epithelial interaction affecting prostate tumor growth and hormonal responsiveness. Cancer Survey 1991; 11: 91-121
  22. Kim MS, Beneficial effect of soy isoflavone on bone loss and hyperlopidemia in ovariectomized rat. Dissertation of Ph. D. Seoul: Seoul National University; 1999
  23. Choi MJ, Kang YJ. Effect of isoflabones on bone mineral dencity and bone mineral content in ovariectomized rats. Korean J Nutr 2006; 39: 236-243
  24. Jang HR. The effect of soy isoflabone supplementation on the factors relating to vascular disease in ovariectomized and hyperlipidemic rats. MS Thesis. Seoul: Seoul National University; 2002
  25. Mituka BM, Rawnsley HN. Clinical biochemical and hemogtologycal reference value in normal experimental animals and normal humans. 2nd edition, Masson, New York. 1987; p.160
  26. Kim WY, Choi HK, Lee HS. The effects of dietary calcium levels on calcium and skelectal metabolism in ovariectomised rats of different ages. Korean J Nutr 1998; 31(4): 55-62
  27. Choi MJ, Jung JW. Effect of soybean protein on BMD and BMC in ovariectomized rats. Korean J Nutr 2005; 38(4): 279-288
  28. Zapata CLV, Donangelo CM, Woodhouse LR, Abrams SA, Spencer EM, King JC, et al. Calcium homeostasis during pragnancy and lactation in Brazilizn women with low calcium intakes: a longitudinal study. Am J Clin Nutr 2004; 80: 417-422 https://doi.org/10.1093/ajcn/80.2.417
  29. Gallagher SK. Biochemiccal makers of bone metabolism as they relate to osteoporosis. Medical Lab Observer 1997; 29: 50-56
  30. Liu G, Peacock M. Age-related changes in serum undercarboxylated osteocalcin and its relationships with bone density, bone quality, and hip fracture. Calci Tissue Int 1998; 62: 286-289 https://doi.org/10.1007/s002239900432
  31. Greger JL, Emery SM. Mineral metabolism and bone strength of rats fed coffee and decaffeinated coffee. J Agric Food Chem 1987; 35: 551-556 https://doi.org/10.1021/jf00076a028
  32. Glajchen N. The effect of chronic caffeine administration on serum makers of bone metabolism and bone histomorphometry in the rat. Calcif Tissue Int 1988; 43: 277-280 https://doi.org/10.1007/BF02556635
  33. Ohta M, Ide K, Cheuk G, Yazdani M, Nakamoto T, Thomas KA. A caffeine diet can alter the mechanical properties of bones of young ovariectomised rats. Ann Nutr Metab 2002; 46: 108-113 https://doi.org/10.1159/000063078
  34. Ried IR, Ibbertson HK. Calcium supplements in prevention of steroid- induced osteoporosis. Am J Clin Nutr 1986; 44: 287-290 https://doi.org/10.1093/ajcn/44.2.287
  35. Choi MJ, JO HJ. Effect of soy protein and isoflabones on bone mineral dencity and bone mineral content in growing female rats. Korean J Nutr 2003; 36: 359-367