Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidant Effect of Crataegi Fructus Extract on the Oxidative Stress of Reactive Oxygen Species in Cultured Human Skin Fibroblast

배양 인체피부섬유모세포에 있어서 활성산소의 산화적 손상에 대한 산사(山査)추출물의 항산화 효과

  • Lim, Young-Mi (Department of School of Medicine, Wonkwang University Graduate School) ;
  • Kim, Byoung-Ryun (Department of Obstetrics and Gynecology, School of Medicine, Wonkwang University) ;
  • Hong, Gi-Youn (Department of Obstetrics and Gynecology, School of Medicine, Wonkwang University)
  • 임영미 (원광대학교 의과대학원) ;
  • 김병륜 (원광대학교 의과대학 산부인과학교실) ;
  • 홍기연 (원광대학교 의과대학 산부인과학교실)
  • Published : 2008.02.25
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Abstract

This study was done to evaluate the antioxidant effect of Crataegi Fructus (CF) extract on the oxidative stress induced by reactive oxygen species (ROS), The human skin fibroblasts (Detroit 551) were cultured with various concentrations of hydrogen peroxide $(H_2O_2)$. The cytotoxicity of $H_2O_2-induced$ oxidative stress was performed by XTT assay for the cell viability according to the dose- and time-dependent treatment. For the protective effect of CF extract on $H_2O_2-mediated$ oxidative stress, cell viability, lactate dehydroganase (LDH) activity, and ferric thiocyanate (FTC) assay for the inhibitive activity of lipid peroxidation on CF extract were carried out. In this study, $H_2O_2-mediated$ oxidative stress was decreased cell viability dose-, and time-dependent manner and increased LDH activity compared with the control in these cultures. In the protective effect, CF extract increased cell viability and decreased LDH activity on $H_2O_2-mediated$ oxidative stress, especially, CF extract has antioxidant effect by the showing the inhibitive activity of lipid peroxidation by FTC assay. From these results, It is suggested that $H_2O_2-mediated$ oxidative stress was highly toxic, and also, CF extract showed the protective effect on $H_2O_2-mediated$ oxidative stress by showing the increased cell viability, decreased LDH activity and lipid peroxidation inhibition in these cultures.

Keywords

References

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