Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Fermentation Increases Antidiabetic Effects of Acanthopanax Senticosusbhpark@chonbuk.ac.kr

발효에 의한 오가피의 항당뇨 활성 촉진

  • Ham, Seong-Ho (Han Seo Pharm. Co. Ltd.) ;
  • Lim, Byung-Lak (H&BT Co, Ltd.) ;
  • Yu, Jia-hua (Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University) ;
  • Ka, Sun-O (Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University) ;
  • Park, Byung-Hyun (Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University)
  • 함성호 (한서제약(주)) ;
  • 임병락 ((주)에이치앤비티 코리아) ;
  • 유가화 (전북대학교 의과학연구소, 의학전문대학원 생화학교실) ;
  • 가선오 (전북대학교 의과학연구소, 의학전문대학원 생화학교실) ;
  • 박병현 (전북대학교 의과학연구소, 의학전문대학원 생화학교실)
  • Published : 2008.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

Extract of Acanthopanax senticosus has recently been demonstrated to possess significant antidiabetic potential, in accordance with the traditional use of this plant as an antidiabetic natural health product. The present study evaluated the effects of fermented extract (FE) of this plant on glucose-stimulated insulin secretion, glucose uptake, and streptozotocin-induced type 1 diabetes model. A 3 h pretreatment with FE prevented $IL-1{\beta}$ and $IFN-{\gamma}$ toxicity in isolated rat islets. However, it did not affect insulin-stimulated glucose uptake in C2C12 myotubes. In addition, pretreatment of mice with FE blocked the destruction of streptozotocin-induced islets and the development of type 1 diabetes. FE reduced blood glucose level, increased insulin secretion, and improved glucose tolerance in streptozotocin-treated mice, whereas nonfermented extract (NFE) had moderate effects. Immunohistochemical staining for insulin clearly showed that pretreatment with FE blocked the STZ-induced islets destruction and restored the number of islet cells that secreted insulin to the level of the control. Although the active principles and their mechanisms of action remain to be identified, FE may nevertheless represent a novel complementary therapy and a source of novel therapeutic agents against type 1 diabetes mellitus.

Keywords

References

  1. Bae, E.A., Han, M.J., Kim, E.J., Kim, D.H. Transformation of ginseng saponins to ginsenoside Rh2 by acids and human intestinal bacteria and biological activities of their transformants. Arch Pharm Res 27: 61-67, 2004 https://doi.org/10.1007/BF02980048
  2. Hasegawa, H. Proof of the mysterious efficacy of ginseng: basic and clinical trials: metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid. J Pharmacol Sci 95: 153-157, 2004 https://doi.org/10.1254/jphs.FMJ04001X4
  3. Akao, T., Kawabata, K., Yanagisawa, E., Ishihara, K., Mizuhara, Y., Wakui, Y., Sakashita, Y., Kobashi, K. Baicalin, the predominant flavone glucuronide of scutellariae radix, is absorbed from the rat gastrointestinal tract as the aglycone and restored to its original form. J Pharm Pharmacol 52: 1563-1568, 2000 https://doi.org/10.1211/0022357001777621
  4. Akao, T., Kobashi, K., Aburada, M. Enzymic studies on the animal and intestinal bacterial metabolism of geniposide. Biol Pharm Bull 17: 1573-1576, 1994 https://doi.org/10.1248/bpb.17.1573
  5. Akao, T., Kobashi, K. Glycyrrhizin ${\beta}$-D-glucuronidase of Eubacterium sp. from human intestinal flora. Chem Pharm Bull (Tokyo) 35: 705-710, 1987 https://doi.org/10.1248/cpb.35.705
  6. Steinmann, G.G., Esperester, A., Joller, P. Immunophar- macological in vitro effects of Eleutherococcus senticosus extracts. Arzneimittelforschung 51: 76-83, 2001
  7. Sui, D.Y., Lu, Z.Z., Li, S.H., Cai, Y. Hypoglycemic effect of saponin isolated from leaves of Acanthopanax senticosus (Rupr. et Maxin.) Harms. Zhongguo Zhong Yao Za Zhi 19: 683-685, 703, 1994
  8. Glatthaar-Saalmuller, B., Sacher, F., Esperester, A. Antiviral activity of an extract derived from roots of Eleutherococcus senticosus. Antiviral Res 50: 223-228, 2001 https://doi.org/10.1016/S0166-3542(01)00143-7
  9. Hibasami, H., Fujikawa, T., Takeda, H., Nishibe, S., Satoh, T., Fujisawa, T., Nakashima, K. Induction of apoptosis by Acanthopanax senticosus HARMS and its component, sesamin in human stomach cancer KATO III cells. Oncol Rep 7: 1213-1216, 2000
  10. Lin, C.C., Huang, P.C. Antioxidant and hepatoprotective effects of Acathopanax senticosus. Phytother Res 14: 489-494, 2000 https://doi.org/10.1002/1099-1573(200011)14:7<489::AID-PTR656>3.0.CO;2-G
  11. Gaffney, B.T., Hugel, H.M., Rich, P.A. The effects of Eleutherococcus senticosus and Panax ginseng on steroidal hormone indices of stress and lymphocyte subset numbers in endurance athletes. Life Sci 70: 431-442, 2001 https://doi.org/10.1016/S0024-3205(01)01394-7
  12. Szolomicki, J., Samochowiec, L., Wojcicki, J., Drozdzik, M. The influence of active components of Eleutherococcus senticosus on cellular defence and physical fitness in man. Phytother Res 14: 30-35, 2000 https://doi.org/10.1002/(SICI)1099-1573(200002)14:1<30::AID-PTR543>3.0.CO;2-V
  13. Yang, J.Y., Lee, K.S., Kim, M.K., Moon, S.K., Kang, M.K., Park, B.H., Kim, J.S., Park, J.W. Effect of Acanthopanax senticosus on lipoprotein lipase in 3T3-L1 adipocytes. Phytother Res 18: 160-163, 2004 https://doi.org/10.1002/ptr.1413
  14. Park, S.Y., Chang, S.Y., Yook, C.S., Nohara, T. New 3,4-seco-lupane-type triterpene glycosides from Acanthopanax senticosus forma inermis. J Nat Prod 63: 1630-1633, 2000 https://doi.org/10.1021/np000277c
  15. Yoshizumi, K., Hirano, K., Ando, H., Hirai, Y., Ida, Y., Tsuji, T., Tanaka, T., Satouchi, K., Terao, J. Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase. J Agric Food Chem 54: 335-341, 2006 https://doi.org/10.1021/jf052047f
  16. Yim, J.S., Kim, Y.S., Moon, S.K., Cho, K.H., Bae, H.S., Kim, J.J., Park, E.K., Kim, D.H. Metabolic activities of ginsenoside Rb1, baicalin, glycyrrhizin and geniposide to their bioactive compounds by human intestinal microflora. Biol Pharm Bull 27: 1580-1583, 2004 https://doi.org/10.1248/bpb.27.1580
  17. Xi, M., Hai, C., Tang, H., Chen, M., Fang, K., Liang, X. Antioxidant and antiglycation properties of total saponins extracted from traditional Chinese medicine used to treat diabetes mellitus. Phytother Res, 2007
  18. Eckburg, P.B., Bik, E.M., Bernstein, C.N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S.R., Nelson, K.E., Relman, D.A. Diversity of the human intestinal microbial flora. Science 308: 1635-1638, 2005 https://doi.org/10.1126/science.1110591
  19. Hooper, L.V., Gordon, J.I. Commensal host-bacterial relationships in the gut. Science 292: 1115-1118, 2001 https://doi.org/10.1126/science.1058709
  20. Hooper, L.V., Wong, M.H., Thelin, A., Hansson, L., Falk, P.G., Gordon, J.I. Molecular analysis of commensal host-microbial relationships in the intestine. Science 291: 881-884, 2001 https://doi.org/10.1126/science.291.5505.881
  21. Kobashi, Y., Matsushima, T. Clinical analysis of recent lower lung field tuberculosis. J Infect Chemother 9: 272-275, 2003 https://doi.org/10.1007/s10156-003-0249-3
  22. Kobashi, Y., Nakajima, M., Niki, Y., Matsushima, T. A case of acute eosinophilic pneumonia due to Sho-saiko-to. Nihon Kyobu Shikkan Gakkai Zasshi 35: 1372-1377, 1997
  23. Kim, E.K., Kwon, K.B., Koo, B.S., Han, M.J., Song, M.Y., Song, E.K., Han, M.K., Park, J.W., Ryu, D.G., Park, B.H. Activation of peroxisome proliferator-activated receptor-${\gamma}$ protects pancreatic $\beta$-cells from cytokine-induced cytotoxicity via NF ${\kappa}B$ pathway. Int J Biochem Cell Biol 39: 1260-1275, 2007 https://doi.org/10.1016/j.biocel.2007.04.005
  24. Hasegawa, H., Sung, J.H., Benno, Y. Role of human intestinal Prevotella oris in hydrolyzing ginseng saponins. Planta Med 63: 436-440, 1997 https://doi.org/10.1055/s-2006-957729
  25. Vuong, T., Martineau, L.C., Ramassamy, C., Matar, C., Haddad, P.S. Fermented Canadian lowbush blueberry juice stimulates glucose uptake and AMP-activated protein kinase in insulin-sensitive cultured muscle cells and adipocytes. Can J Physiol Pharmacol 85: 956-965, 2007 https://doi.org/10.1139/Y07-090
  26. Liu, T.P., Lee, C.S., Liou, S.S., Liu, I.M., Cheng, J.T. Improvement of insulin resistance by Acanthopanax senticosus root in fructose-rich chow-fed rats. Clin Exp Pharmacol Physiol 32: 649-654, 2005 https://doi.org/10.1111/j.0305-1870.2005.04245.x
  27. De Vos, P., Lefebvre, A.M., Miller, S.G., Guerre-Millo, M., Wong, K., Saladin, R., Hamann, L.G., Staels, B., Briggs, M.R., Auwerx, J. Thiazolidinediones repress ob gene expression in rodents via activation of peroxisome proliferator-activated receptor gamma. J Clin Invest 98: 1004-1009, 1996 https://doi.org/10.1172/JCI118860