Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Apoptotic Effect of Vitisin A from Vitis Amurensis against MES-SA Uterine Cancer Cells

왕머루 포도에서 분리한 Vitisin A의 자궁암주에 대한 자멸사 효과

  • Lim, Jeong-Han (Graduate School of East-West Medical Science, Kyunghee University) ;
  • Lee, Hyo-Jeong (College of Oriental Medicine, Kyunghee University) ;
  • Lee, Eun-Ok (College of Oriental Medicine, Kyunghee University) ;
  • Lee, Hyo-Jung (College of Oriental Medicine, Kyunghee University) ;
  • Kwon, Hee-Young (College of Oriental Medicine, Kyunghee University) ;
  • Shim, Bum-Sang (College of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (College of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medical Science, Kyunghee University, College of Oriental Medicine, Kyunghee University)
  • 임정한 (경희대학교 동서의학대학원) ;
  • 이효정 (경희대학교 한의과대학) ;
  • 이은옥 (경희대학교 한의과대학) ;
  • 이효정 (경희대학교 한의과대학) ;
  • 권희영 (경희대학교 한의과대학) ;
  • 심범상 (경희대학교 한의과대학) ;
  • 안규석 (경희대학교 한의과대학) ;
  • 김성훈 (경희대학교 동서의학대학원, 경희대학교 한의과대학)
  • Published : 2008.04.25
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Abstract

The cytotoxic characteristics of Vitsin A isolated from Vitis amurensis L. were examined in human colorectal, breast, uterine and renal cancer cells. Vitsin A showed good cytotoxicity against various cancer cells with $IC_{50}$ of $1\;{\sim}\;30\;{\mu}M$. Among them, Vitisin A exhibited strongest cytotoxic effect against MES-SA cells with $IC_{50}$ of 1.11 ${\mu}M$ by SRB assay. To verify whether the cytotoxicity of Vitisin A may be associated with apoptosis, TdT-mediated-dUTP Nick-End Labeling (TUNEL) assay and cell cycle analysis were performed in MES-SA cells. Apoptotic bodies were observed in Vitisin A treated MES-SA cells by TUNEL assay. Also, Vitisin A effectively increased the portion of $sub-G_1$ DNA content by flow cytometric analysis. Taken together, these findings suggest that the cytotoxicity of Vitisin A against MES-SA cells is chiefly mediated by apoptosis.

Keywords

References

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