Reproductive and Developmental Biology

  • 제32권1호
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  • Pages.45-49
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  • 2008
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Co-expression of Human Proteins (IL-10, TPO and/or Lactoferrin) into Milk of Cross-Breed Transgenic Mouse

  • Zheng, Zhen-Yu (The College of Animal Husbandary and Veterinary, Henan Agricultural University) ;
  • Lee, Hyo-Sang (Center for Regenerative Medicine, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Oh, Keon-Bong (Center for Regenerative Medicine, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Koo, Deog-Bon (Center for Regenerative Medicine, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Han, Yong-Mahn (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST)) ;
  • Lee, Kyung-Kwang (Center for Regenerative Medicine, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 발행 : 2008.03.31
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초록

We have previously produced transgenic (TG) mice expressing the human lactoferrin (hLF), interleukin-10 (hIL-10), and thrombopoietin (hTPO) proteins in the milk. In this study, we examined whether simple crossbreeding between two kids of a single transgenic mouse can produce double transgenics co-expressing two human proteins.. The hLF male, and the hIL-10 male were crossbred with the hIL-10 and hTPO females, and the hTPO female, respectively. PCR analysis for genotyping showed 32%, 23% and 24% double transgenic rates for hLF/hIL-10, hLF/hTPO, and hIL-10/hTPO transgenes, respectively. We analyzed the expression levels of the human proteins from double transgenic mice and compared those with their single transgenic siblings. All double transgenic co-expressed two human proteins at comparable levels to singles', unless hTPO was not co-expressed: for hLF, 1.1 mg/ml in hLF/hIL-10, whereas 0.5 mg/ml in hLF/hTPO; for hIL-10, 4.1 mg/ml in hIL-10/hLF, whereas 1.4 mg/ml in hIL-10/hTPO. Ihe downregulation of hTPO to half level of singles' was observed in double transgenic mice. The possible reason why hTPO co-expressed might lead to down-regulation of another human protein was discussed. These results suggested that double transgenic generated by crossbreeding between two singles' could be useful system for bioreactor.

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