소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp.의 분리 및 동정

Isolation and Identification of Micromonospora sp. Showing Nematocidal Activity Against Pine Wood Nematode

  • 박동진 (한국생명공학연구원 기능대사물질연구센터) ;
  • 이재찬 (한국생명공학연구원 기능대사물질연구센터) ;
  • 김판경 (한국생명공학연구원 기능대사물질연구센터) ;
  • 김창진 (한국생명공학연구원 기능대사물질연구센터)
  • Park, Dong-Jin (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Chan (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Pan-Kyung (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Jin (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2008.03.31

초록

소나무재선충에 대한 살선충 활성을 나타내는 방선균의 탐색 및 동정을 위하여 2000여개의 방선균배양액 라이브러리로부터 살선충 활성을 탐색하였다. 현재 소나무재선충에 대한 생물학적 방제제로 사용되고 있는 Streptomyces avermitilis 균주 배양액의 살선충 활성과 비교하여 선발한 결과, 강력한 살선충 활성을 나타내는 균주 AW050027을 균주를 최종 선발하였다. 선발군주에 대하여 형태학적, 배양학적, 생리학적 특성 분석 및 분자계통분류학적 분석을 한 결과 선발군주는 Micromonospora coriariae $NAR01^T$ 균주와 98.9%의 상동성을 가진 균주로 동정되었다.

For the isolation of Actinomycetes showing nematocidal activity against Pine wood nematode, Bursaphelenchus xylophilus, about 2000 culture broth of Actinomycetes were tested and their activity were compared with that of Streptomyces avermitilis resulting a selected strain AW050027. The cultural, morphological and physiological analysis was performed for the identification of a selected strain. Phylogenetic analyses based on 165 rDNA gene sequences showed that the selected strain AW050027 belonged to the genus Micromonospora and M. corioriae $NAR01^T$ was the closest neighbors, sharing 98.9% 165 rDNA gene sequence similarity.

키워드

참고문헌

  1. Cole, J. R., B. Chai, R. J. Farris, Q. Wang, A. S. Kulam-Syed- Mohideen, D. M. McGarrell, A. M. Bandela, E. Cardenas, G. M. Garrity & J. M. Tiedje (2007). The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res 35 (Database issue): D169-D172; doi: 10.1093/nar/gkl889
  2. Edward, O. R. and M. J. Linit (1992) Transmission of Bursaphelenchus xylophilus through cviposition wounds of Monachamus alternatus (Coleoptera : Cerambycidae). J. Nematol. 24:133-139
  3. Felsenstein, J. (2002). PHYLIP (phylogeny inference package), version 3.6a, Seattle: Department of Genetics, University of Washington, Seattle, WA, USA
  4. Holt, J. G., N. R. Kriege, P. H. A. Snea, J. T. Staley and S. T. Williams (1994) Bergey's Manual of Determinative Bacteriology, pp. 605-703. 9th ed Williams & Wilkins, Baltimore
  5. ISCC-NBS Color-Name Charts Illustrated with Centroid Colors. National Bureau of Standards, U.S.A
  6. Kawazu, K., H. Zhang, H. Tamashita and H. Kanzaki (1996) Relationship between the pathogenicity of the pine wood nematode, Bursaphelenchus xylophilus, and phanylacetic acid production. Biosci. Biotechnol. Biochem. 60:1430-1415 https://doi.org/10.1271/bbb.60.1430
  7. Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16:111-120 https://doi.org/10.1007/BF01731581
  8. Lane, D. J. (1991) 16S/23S rDNA sequencing, In Nucleic Acid Techniques in Bacterial Systematics, pp. 115-175. Edited by E. Stackebrandt & M. Goodfellow, John Wiley and Sons, Chichester, United Kingdom
  9. Lechevalier, M. P. and H. Lechevalier (1970) Chemical composition as a criterion in the classification of aerobic actinomycetes. Int. Syst. Bacteriol. 20:435-443 https://doi.org/10.1099/00207713-20-4-435
  10. Mamiya, Y. and N. Enda (1972) Transmission of Bursaphelenchus lignicolus (Nematoda : Aphelenchoididae) by Monachamus alternatus (Coleoptera : Cerambycidae). Nematologica 18: 159-162 https://doi.org/10.1163/187529272X00395
  11. Morimoto, K. and A. Iwasaki (1972) Role of Monachamus alternatus (Coleoptera : Cerambycidae) as a vector of Bursaphelenchus lignicolus (Nematoda : Aphelenchoididae). J. Jap. For. Soc. 54:177-183
  12. Saitou, N. & M. Nei (1987) The neighbor-joining method : a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425
  13. Shirling, E. B. and D. Gottlieb (1966) Methods for characterization of Streptomyces species. Int. J. Syst. Bacterial. 16:313-340 https://doi.org/10.1099/00207713-16-3-313
  14. Thompson, J. D., D. G. Higgins & T. J. Gibson (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  15. Williams, S. T. and F. L. Davis (1967) Use of a scanning electron microscope for the examination of Actinomycetes. J. Gen. Microbiol. 48:171-177 https://doi.org/10.1099/00221287-48-2-171
  16. Wingfield, M. J. and R. B. Blanchette (1983) The pine-wood nematode, Bursaphelenchus xylophilus, in Minnesota and Wisconsin: insect associates and transmission studies. Can. J. for. Res. 13:1068-1076 https://doi.org/10.1139/x83-143
  17. Zao, B. G. and F. Lin (2005) Mutualistic symbiosis between Bursaphelenchus xylophilus and bacteria of genus Pseudomonas. For. Path. 35:339-345 https://doi.org/10.1111/j.1439-0329.2005.00417.x
  18. 산림청, 국립산림과학원 (2004) 소나무AIDS 소나무재선충병. 산림과학속보. '04-06