한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제37권5호
  • /
  • Pages.542-547
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  • 2008
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

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한약재의 물 추출물이 당대사 관련 효소와 항산화 활성에 관한 연구

A Study on the Glucose-regulating Enzymes and Antioxidant Activities of Water Extracts from Medicinal Herbs

  • 최면 (강원대학교 식물생명공학) ;
  • 김대중 (강원대학교 식물생명공학) ;
  • 이현주 (강원대학교 식물생명공학) ;
  • 유진균 (강원대학교 식물생명공학) ;
  • 서동주 (강원대학교 식물생명공학) ;
  • 이준희 (강원대학교 생명공학부) ;
  • 정미자 (강원대학교 BK21 사업단(뉴트라슈티컬바이오))
  • Choe, Myeon (Dept. of Plant Biotechnology, Kangwon National University) ;
  • Kim, Dae-Jung (Dept. of Plant Biotechnology, Kangwon National University) ;
  • Lee, Hyeon-Ju (Dept. of Plant Biotechnology, Kangwon National University) ;
  • You, Jin-Kyoun (Dept. of Plant Biotechnology, Kangwon National University) ;
  • Seo, Dong-Joo (Dept. of Plant Biotechnology, Kangwon National University) ;
  • Lee, Joon-Hee (Division of Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University)
  • 발행 : 2008.05.31
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초록

본 연구에서는 제2형 당뇨병를 가진 GK 흰쥐(Goto-Kakizaki) 간에서 추출한 cytosol과 심장에서 추출한 mitochondria를 이용한 모델계에서 당대사 관련 효소인 glucokinase glucokinase(GCK), pyruvate dehydrogenase(PDH), acetyl-CoA carboxylase(ACC) 및 glucosidase 활성에 대한 한약재의 물 추출물의 항당뇨 효과를 연구하였다. 그리고 역시 그들의 물 추출물의 free radical 소거활성을 DPPH 방법으로 알아보았다. Free radical 소거활성은 산수유(CF), 목단피(MDB), 천화분(CHB) 그리고 산약(SY)의 물 추출물이 강했고 반면에 백목령(BBR), 숙지황(SGH)과 택사(TS)는 낮은 소거작용을 나타내었다. 간 cytosol의 GCK 활성은 산수유(CF)와 천화분(CHB)에서 다른 추출물보다 더 강했다. 심장미토콘드리아의 PDH 활성은 택사(TS)를 제외하고 모든 추출물에서 대조군과 비교하여 높았다. 간 cytosol의 ACC 활성은 대조군보다 산수유(CF), 천화분(CHB), 숙지황(SGH), 택사(TS) 그리고 산약(SY) 추출물에서 높았다. 산수유(CF), 백복령(BBR) 및 목단피(MDB) 추출물은 ${\alpha}$-glucosidase 활성 감소를 유도했다. 따라서 모든 추출물은 혈당 상승을 억제할 수 있는 항당뇨 기능성식품이나 약품 개발을 위한 기능성 천연 소재로 이용될 수 있을 가능성을 제시하였다.

The anti-diabetic effects of water extracts (WE) from medicinal herbs on hepatic glucose-regulating enzymes, such as glucokinase (GCK), pyruvate dehydrogenase (PDH), acetyl-CoA carboxylase (ACC) and ${\alpha}$-glucosidase, were studied using the cytosol fraction in liver and mitochondia fraction in heart of a type II diabetic animal (GK rat, Goto-Kakizaki). The free radical scavenging activity of water extracts by DPPH method was also tested. We found that free radical scavenging activity was strong in Corni fructu (CF), Mokdan Bark (MDB), Chenhwabon (CHB) and Sanyack (SY), while that of Backbocreng (BBR), Shuckgihwang (SGH) and Taecsa (TS) was lower. For GCK activity in cytosol of liver, CF and CHB had a more effective activity than other extracts. PDH activity in mitochondria fraction of heart was higher in all of the extracts, expect for the TS extract, than in the control. ACC activity in cytosol fraction of liver was significantly higher in the CF, CHB, SGH, TS and SY extracts than in the control. CF, BBR and MDB led to a decrease in the ${\alpha}$-glucosidase activity. Therefore, these results suggest that all of the extracts may be used as functional material in the development as anti-diabetic functional food and medicine.

키워드

참고문헌

  1. Wahren J, Felig P, Cerasi E, Luft R. 1972. Splanchnic and peripheral glucose amino acid metabolism in diabetes mellitus. J Clin Invest 51: 870-876 https://doi.org/10.1172/JCI106989
  2. Saudek CD, Eder HA. 1979. Lipid metabolism in diabetes mellitus. Am J Med 66: 843-849 https://doi.org/10.1016/0002-9343(79)91126-4
  3. Jung UJ, Lee MK, Park YB, Kang MA, Choi MS. 2006. Effect of citrus flavonoids on lipid metabolism and glucose- regulating enzyme mRNA levels in type-2 diabetic mice. Int J Biochem Cell Biol 38: 1134-1145 https://doi.org/10.1016/j.biocel.2005.12.002
  4. Park SA, Choi MS, Kim MJ, Jung UJ, Kim HJ, Park KK, Noh HJ, Park HM, Park YB, Lee JS, Lee MK. 2006. Hypoglycemic and hypolipidemic action of Du-zhong (Eucommia ulmoides Oliver) leaves water extract in C57BL/KsJ-db/db mice. J Ethnopharmacol 107: 412-417 https://doi.org/10.1016/j.jep.2006.03.034
  5. Bailey CJ. 1999. Insulin resistance and antidiabetic drugs. Biochem Pharmacol 58: 1511-1520 https://doi.org/10.1016/S0006-2952(99)00191-4
  6. Zhang BB, Moller DE. 2000. New approaches in the treatment of type 2 diabetes. Curr Opin Chem Biol 4: 461-467 https://doi.org/10.1016/S1367-5931(00)00103-4
  7. Song Y, Wang J, Li Y, Du Y, Arteel GE, Saari JT, Kang YJ, Cai L. 2005. Cardiac metallothionein synthesis in streptozotocin- induced diabetic mice, and its protection against diabetes-induced cardiac injury. Am J Patbol 167: 17-26 https://doi.org/10.1016/S0002-9440(10)62949-5
  8. Cai L, Kang YJ. 2001. Oxidative stress and diabetic cardiomyopathy: a brief review. Cardiovasc Toxicol 1: 181- 193 https://doi.org/10.1385/CT:1:3:181
  9. Hogeboom GH, Schneider WC, Pallade GE. 1948. Cytochemical studies of mammalian tissues. I. Isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submicroscopic particulate matter. J Biol Chem 172: 619-635
  10. Singh N, Rajini PS. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85: 611-616 https://doi.org/10.1016/j.foodchem.2003.07.003
  11. Sharma C, Manjechwar R, Weinhouse S. 1963. Effects of diet and insulin on glucose-adensine triphoshate phosphotransferases of rat liver. J Biol Chem 238: 3840-3845
  12. Furuta S, Hashimoto T. 1982. Pyruvate dehydrogenase complex from pigeon breast muscle. Methods Enzymol 89: 414-420 https://doi.org/10.1016/S0076-6879(82)89072-1
  13. Tanabe T, Nakanishi S, Hashimoto T, Ogiwara H, Nikawa J, Numa S. 1981. Acetyl-CoA carboxylase from rat liver. Methods Enzymol 71: 5-15 https://doi.org/10.1016/0076-6879(81)71003-6
  14. Xu XM, Jun JY, Jeong IH. 2007. A study on the antioxidant activity of Hae-Songi mushroom (Hypsizigus marmoreus) hot water extracts. J Korean Sci Food Sic Nutr 36: 1351- 1357 https://doi.org/10.3746/jkfn.2007.36.11.1351
  15. Kim DI, Lee SH, Hur EY, Cho SM, Park HJ. 2005. Screening of natural plant resources with acetylcholinesterase inhibition and antioxidant activity. J Korean Soc Food Sci Nutr 32: 427-432 https://doi.org/10.3746/jkfn.2005.34.3.427
  16. Kim SJ, Kweon DH, Lee JH. 2006. Investigation of antioxidant activity and stability of ethanol extracts of licorice root (Glycyrrhiza glabra). Korean J Food Sci Technol 38: 584-588
  17. Ferre T, Pujol A, Riu E, Bosch F, Valera A. 1996. Correction of diabetic alterations by glucokinase. Proc Natl Acad Sci USA 93: 7225-7230 https://doi.org/10.1073/pnas.93.14.7225
  18. Munoz MC, Barbera A, Dominguez J, Fermandez-Alvarez J, Gomis R, Guinovart JJ. 2001. Effects of tungstate, a new potential oral antidiabetic agent, in Zucker diabetic fatty rats. Diabetes 50: 131-138 https://doi.org/10.2337/diabetes.50.1.131
  19. Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM. 1999. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic $\beta$- cell-specific gene knock-outs using Cre recombinase. J Biol Chem 274: 305-315 https://doi.org/10.1074/jbc.274.1.305
  20. Iynedjian PB, Gjinovei A, Renold AE. 1988. Stimulation by insulin of glucokinase gene transcription in liver of diabetic rats. J Biol Chem 263: 740-744
  21. Shimizu T, Parker JC, Najafi H, Matschinsky FM. 1988. Control of glucose metabolism in pancreatic $\beta$-cells by glucokinase, hexokinase and phosphofructokinase; model study with cell lines derived from $\beta$-cells. Diabetes 37: 1524-1530 https://doi.org/10.2337/diabetes.37.11.1524
  22. Matschinsky FM. 1990. Glucokinase as glucose sensor and metabolic signal generator in pancreatic $\beta$-cells and hepatocytes. Diabetes 39: 647-652 https://doi.org/10.2337/diabetes.39.6.647
  23. Lee HA, Sim HS, Choi KJ, Lee HB. 1998. Hypoglycemic action of red ginseng components (II): investigation of the effect of fat soluble fraction from red ginseng on enzymes related to glucose metabolism in cultured rat hepatocytes. Korean J Ginseng Sci 22: 51-59
  24. Kim HS, Ro YJ, Cho M. 2005. Effect of Cordyceps militaris on key enzymes of carbohydrate metabolism. J Korean Soc Food Sci Nutr 34: 1531-1535 https://doi.org/10.3746/jkfn.2005.34.10.1531
  25. Hwang JY, Han JS. 2007. Inhibitory effects of Sasa borealis leaves extracts on carbohydrate digestive enzymes and postprandial hyperglycemia. J Korean Soc Food Sci Nutr 36: 989-994 https://doi.org/10.3746/jkfn.2007.36.8.989
  26. Kim JI, Kang MJ, Bae SY. 2003. Hypoglycemic effect of the methanol extract of soybean sprout in streptozotocin- induced diabetic rats. J Korean Soc Food Sci Nutr 32: 921-925 https://doi.org/10.3746/jkfn.2003.32.6.921
  27. Ji ST, Lee SJ, Lee KE, Son YT, Chung YK. 2002. Inhibitory effect of extracts from Paeoniae radix on postprandial hyperglycemia. J Korean Soc Food Sci Nutr 31: 131-135 https://doi.org/10.3746/jkfn.2002.31.1.131
  28. Lee SO, Kim MJ, Kim DG, Choi HJ. 2005. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. J Korean Soc Food Sci Nutr 32: 139-147 https://doi.org/10.3746/jkfn.2005.34.2.139

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  9. Saeng-Kankunbi-Tang (生肝健脾汤) protects liver against oxidative damage through activation of ERK/Nrf2 pathway vol.22, pp.8, 2016, https://doi.org/10.1007/s11655-016-2466-5
  10. Change of Antioxidant Activities in Preparing Freeze Dried Wild Vegetable Block for the Long-term Storage vol.38, pp.12, 2009, https://doi.org/10.3746/jkfn.2009.38.12.1649
  11. Biological Activities of Dried Garlic, Red Ginseng and Their Mixture vol.38, pp.12, 2009, https://doi.org/10.3746/jkfn.2009.38.12.1633
  12. Biological Activity of Browning Compounds from Processed Garlics Separated by Dialysis Membrane vol.40, pp.3, 2011, https://doi.org/10.3746/jkfn.2011.40.3.357
  13. The Antioxidative Effect of Ethanol Extracts from Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium Linn, and Lonicera japonica vol.23, pp.5, 2013, https://doi.org/10.5352/JLS.2013.23.5.643
  14. Effect of fermented water plantain on growth performance, meat composition, oxidative stability, and fatty acid composition of broiler vol.162, 2014, https://doi.org/10.1016/j.livsci.2014.01.016
  15. The Effects of Soybean Protopectinase on Melanin Biosynthesis vol.42, pp.3, 2013, https://doi.org/10.3746/jkfn.2013.42.3.355
  16. Evaluation of fermented Alisma canaliculatum with probiotics as potential feed additives for finishing Hanwoo (Bos taurus coreanae) steers vol.54, pp.8, 2014, https://doi.org/10.1071/AN13160
  17. Increased glucose metabolism and alpha-glucosidase inhibition in Cordyceps militaris water extract-treated HepG2 cells vol.11, pp.3, 2017, https://doi.org/10.4162/nrp.2017.11.3.180
  18. Inhibition of Browning and Preference Improvements of Dioscorea batatas through the Addition of Sugar Alcohols and Organic Acids vol.14, pp.3, 2009, https://doi.org/10.3746/jfn.2009.14.3.220
  19. Effect of Removed Peel from Sweet Persimmon on Nutritional Ingredients and Antioxidant Activities vol.39, pp.10, 2010, https://doi.org/10.3746/jkfn.2010.39.10.1495
  20. Growth Performance, Meat Yield, Oxidative Stability, and Fatty Acid Composition of Meat from Broilers Fed Diets Supplemented with a Medicinal Plant and Probiotics vol.25, pp.8, 2012, https://doi.org/10.5713/ajas.2012.12090
  21. Enhancing the Organoleptic and Functional Properties of Jujube by a Quick Aging Process vol.18, pp.1, 2013, https://doi.org/10.3746/pnf.2013.18.1.050
  22. Physiological activities of natural color powders and their mixtures vol.23, pp.1, 2016, https://doi.org/10.11002/kjfp.2016.23.1.80
  23. Effect of a Combined Extract of Orostachys japonicus with Medicinal Plants on the Lipid Composition of the Liver and Kidney from Streptozotocin-induced Diabetic Rats vol.41, pp.4, 2012, https://doi.org/10.3746/jkfn.2012.41.4.510
  24. α-Glucosidase Inhibition Activity of Methanol Extracts and Fractions Obtained from Three Dryopteridaceae Species vol.21, pp.4, 2013, https://doi.org/10.7783/KJMCS.2013.21.4.301
  25. Anti-Obesity Effects of Foeniculum fructus Water Extract vol.39, pp.11, 2010, https://doi.org/10.3746/jkfn.2010.39.11.1604
  26. Change of Antioxidant Activities in Carrots (Daucus carota var. sativa) with Enzyme Treatment vol.42, pp.2, 2013, https://doi.org/10.3746/jkfn.2013.42.2.262
  27. Nutritional Properties and Biological Activities of Artemisia annua L. vol.40, pp.2, 2011, https://doi.org/10.3746/jkfn.2011.40.2.163
  28. Effects of medicinal herb water extracts on expression of hepatic glucokinase, pyruvate dehydrogenase and acetyl-CoA carboxylase mRNA vol.46, pp.2, 2013, https://doi.org/10.4163/kjn.2013.46.2.119
  29. Hypoglycemic and Hypolipidemic Effects of Orostachys japonicus with Medicinal Herbs in Streptozotocin-Induced Diabetic Rats vol.42, pp.4, 2013, https://doi.org/10.3746/jkfn.2013.42.4.587
  30. Effects of Medicinal Plant Water Extracts on Expression of Anti-diabetic Enzymes mRNA vol.42, pp.7, 2013, https://doi.org/10.3746/jkfn.2013.42.7.1008
  31. Development of Optimization Mixture Tea prepared with Roasting Mulberry Leaf and Fruit vol.29, pp.6, 2016, https://doi.org/10.9799/ksfan.2016.29.6.1040
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  33. Leuconostoc mesenteroidesies 균주를 이용한 여주 추출물 발효 및 생산물의 생리활성 특성 vol.35, pp.4, 2008, https://doi.org/10.12925/jkocs.2018.35.4.1250
  34. Paenibacillus kribbensis AM49로 발효시킨 택사의 항산화 활성 및 NO 생성 효과 vol.36, pp.4, 2008, https://doi.org/10.6116/kjh.2021.36.4.23.