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The Korean Association of Immunobiologists (대한면역학회)
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Non-specific Immune Response of Rainbow Trout (Oncorhynchus Mykiss) by Dietary Heat-inactivated Potential Probiotics

  • Published : 2008.09.30
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Abstract

Background: The effects of the dietary administration of two heat-inactivated whole bacteria from the Vibrionaceae family, singly or combined, on innate immune response of the rainbow trout were studied. The two bacteria (Pdp11 and 51M6), which were obtained from the skin of rainbow trout, showed in vitro characteristics that suggested they could be considered as potential fish probiotics. Methods: The fish were fed four different diets: control (non-supplemented), or diets supplemented with heat-inactivated bacteria at $10^8$ cfu/g Pdp11, $10^8$ cfu/g 51M6 or with $0.5{\times}10^8$ cfu/g Pdp11 plus $0.5{\times}10^8$ cfu/g 51M6 for 4 weeks. Six fish were sampled at weeks 1, 2, 3 and 4, and then the main humoral (natural haemolytic complement activity and serum peroxidase content) and cellular innate immune responses (leucocyte peroxidase content, phagocytosis, respiratory burst and cytotoxicity) were evaluated. Results: The serum peroxidase content and the natural haemolytic complement activity increased with time, reaching the highest values in the third and fourth weeks of feeding, respectively. The phagocytic ability of specimens fed the mixture of the two inactivated bacteria was significantly higher than in the controls after 2 and 3 weeks of treatment. The same activity increased significantly in rainbow trout fed the Pdp11 diet for 2 weeks or the 51M6 diet for 3 weeks. Respiratory burst activity was unaffected by all the experimental diets at all times assayed. Cytotoxic activity had significantly increased after 3 weeks in fish fed the 51M6 diet. Conclusion: Our results demonstrated the usefulness of incorporating inactivated probiotic bacteria into fish diets.

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References

  1. Gatesoupe FJ: The use of probiotics in aquaculture. Aquaculture 189;147-165, 1999
  2. Verschuere L, Rombaut G, Sorgeloos P, Verstraete W: Probiotic bacteria as biological control agents in aquaculture. Microbiol & Mol Biol Rev 64;655-671, 2000 https://doi.org/10.1128/MMBR.64.4.655-671.2000
  3. Schrezenmeir J, de Vrese M: Probiotics, prebiotics and symbiotics - approaching a definition. The Amer J of Clin Nut 73;361-364, 2001 https://doi.org/10.1093/ajcn/73.2.361s
  4. Bly JE, Quiniou SMA, Lawson LA, Clem LW: Inhibition of Saprolegnia pathogenic for fish by Pseudomonas fluorescens. J of Fish Dis 20;35-40, 1997 https://doi.org/10.1046/j.1365-2761.1997.d01-104.x
  5. Gomez-Gil B, Roque A, Tumbull JF: The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture 191;259-270, 2000 https://doi.org/10.1016/S0044-8486(00)00431-2
  6. Spanggaard B, Huber I, Nielsen J, Sick EB, Pipper CB, Martinussen: The probiotic potential against vibriosis of the indigenous microflora of rainbow trout. Environ Microbiol 3;755-765, 2001 https://doi.org/10.1046/j.1462-2920.2001.00240.x
  7. Raida MK, Larsen JL, Nielsen ME, Buchmann K: Enhanced resistance of rainbow trout, Oncorhynchus mykiss (Walbaum), against Yersinia ruckeri challenge following oral administration of Bacillus subtilis and B. licheniformis (BioPlus2B). J of Fish Dis 26;495-498, 2003 https://doi.org/10.1046/j.1365-2761.2003.00480.x
  8. Al-Harbi AH, Naim Uddin M: Seasonal variation in the intestinal bacterial flora of hybrid tilapia (Oreochromis niloticusxOreochromis aureus) cultured in earthen ponds in Saudi Arabia. Aquaculture 229;37-44, 2004 https://doi.org/10.1016/S0044-8486(03)00388-0
  9. Nikoskelainen S, Ouwehand AC, Bylund G, Salminen S, Lilius EM: Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish & Shellfish Immunol 15;443-452, 2003 https://doi.org/10.1016/S1050-4648(03)00023-8
  10. Villamil L, Figueras A, Planas M, Novoa B: Control of Vibrio alginolyticus in Artemia culture by treatment with bacterial probiotics. Aquaculture 219;43-56, 2003 https://doi.org/10.1016/S0044-8486(02)00515-X
  11. Panigrahi A, Kiron V, Puangkaew J, Kobayashi T, Satoh S, Sugita H: The viability of probiotic bacteria as a factor influencing the immune response in rainbow trout Oncorhynchus mykiss. Aquaculture 243;241-254, 2005 https://doi.org/10.1016/j.aquaculture.2004.09.032
  12. Salinas I, Cuesta A, Esteban MA, Meseguer J: Dietary administration of Lactobacillus delbrüeckii and Bacillus subtilis, single or combined, on gilthead seabream cellular innate immune responses. Fish & Shellfish Immunol 19; 67-77, 2005 https://doi.org/10.1016/j.fsi.2004.11.007
  13. Naidu AS, Bidlack WR, Clemens AR: Probiotic spectra of lactic acid bacteria (LAB). Critic Rev in Food Sci & Nut 39;13-129, 1999 https://doi.org/10.1080/10408699991279187
  14. Salminen S, Ouwehand AC, Benno Y, Lee YK: Probiotics:how should they be defined? Trends in Food Sci Technol 10;107-110, 1999 https://doi.org/10.1016/S0924-2244(99)00027-8
  15. Irianto A, Robertson PA, Austin B: Oral administration of formalin-inactivated cells of Aeromonas hydrophila A3-51 controls infection by atypical A. salmonicida in goldfish, Carassius auratus (L.). J of Fish Dis 26;117-120, 2003 https://doi.org/10.1046/j.1365-2761.2003.00439.x
  16. Irianto A, Austin B: Use of dead probiotic cells to control furunculosis in rainbow trout, Onchorhynchus mykiss (Walbaum). J of Fish Dis 26;59-62, 2003 https://doi.org/10.1046/j.1365-2761.2003.00414.x
  17. Chabrillon M, Rico RM, Balebona MC, Morinigo M: Adhesion to sole Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp. Piscicida. J of Fish Dis 28;229-237, 2005 https://doi.org/10.1111/j.1365-2761.2005.00623.x
  18. Esteban MA, Mulero V, Munoz J, Meseguer J: Methodological aspects of assessing phagocytosis of Vibrio anguillarum by leucocytes of gilthead seabream (Sparus aurata L.) by flow cytometry and electron microscopy. Cell & Tissue Research 293;133-141, 1998 https://doi.org/10.1007/s004410051105
  19. Ortuno J, Esteban MA, Mulero V, Meseguer J. Methods for Studying the Haemolytic, Chemoattractant and Opsonic Activities of Seabream (Sparus aurata L.) Serum, Methodology in Fish Diseases Research. Albion Press, Aberdeen, United Kingdom; 1998
  20. Quade MJ, Roth JA: A rapid, direct assay to measure degranulation of bovine neutrophil primary granules. Vet Imm & Immunopathol 58;239-248, 1997 https://doi.org/10.1016/S0165-2427(97)00048-2
  21. Rodriguez A, Esteban MA, Meseguer J: Phagocytosis and peroxidase release by seabream (Sparus aurata L.) leucocytes in response to yeast cells. Anatomical Record A 272; 415-423, 2003
  22. Secombes CJ, Chung S, Jeffries AH: Superoxide anion production by rainbow trout macrophages detected by the reduction of ferricytochrome C. Dev Comp Immunol 12; 201-206, 1998 https://doi.org/10.1016/0145-305X(88)90038-9
  23. Cuesta A, Esteban MA, Meseguer: J Natural cytotoxic activity of gilthead seabream (Sparus aurata L.) leucocytes. Assessment by flow cytometry. Vet Imm & Immunopathol 71;161-171, 1999 https://doi.org/10.1016/S0165-2427(99)00063-X
  24. Panigrahi A, Kiron V, Kobayashi T, Puangkaew J, Satoh S, Sugita H: Immune responses in rainbow trout Oncorhynchus mykiss induced by a potential probiotic bacteria Lactobacillus rhamnosus JCM 1136. Vet Immunol & Immunopathol 102;379-388, 2004 https://doi.org/10.1016/j.vetimm.2004.08.006
  25. Irianto A, Austin B: Use of probiotics to control furunculosis in rainbow trout Oncorhynchus mykiss (Walbaum). J of Fish Dis 25;333-342, 2002 https://doi.org/10.1046/j.1365-2761.2002.00375.x
  26. Villamil L, Tafalla C, Figueras A, Novoa B: Evaluation of immunomodulatory effects of lactic acid bacteria in turbot (Scophthalmus maximus). Clin & Diag Lab Immunol 9;1318-1323, 2002
  27. Timmerman HM, Koning CJM, Mulder L, Rombout FM, Beynen AC: Monostrain, multistrain and multispecies probiotics - a comparison of functionality and efficacy. Inter J of Food Microbiol 96;219-233, 2004 https://doi.org/10.1016/j.ijfoodmicro.2004.05.012