• Title/Summary/Keyword: rainbow trout

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Cloning and Expression Analysis of Gonadogenesis-associated Gene SPATA4 from Rainbow Trout (Oncorhynchus mykiss)

  • Liu, Bowen;Liu, Shangfeng;He, Shan;Zhao, Ying;Hu, Hongxia;Wang, Zhao
    • BMB Reports
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    • v.38 no.2
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    • pp.206-210
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    • 2005
  • Gonadogenesis is a complicated process which involves multi-gene interactions. A rainbow trout (Oncorhynchus mykiss) gene spermatogenesis associated 4 (SPATA4) was cloned and characterized from adult rainbow trout testis. The cDNA sequence of rainbow trout SPATA4 contains an open reading frame of 1, 081 nucleatides encoding a putative protein of 259 amino acids. The putative protein from rainbow trout shares a 76.8% homology with zebrafish SPATA4. No trans-membrane regions or signal peptide were detected using bioinformatics methods. Subcellular localization analysis revealed that rainbow trout SPATA4 was a nuclear protein with highest possibility (39.1%). Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the distribution of rainbow trout SPATA4 in eleven organs of adult rainbow trout. The result demonstrated that this gene express specifically in testis and slight amount of expression was detected in ovary. Further analysis of SPATA4 characterization and function in rainbow trout may provide insight into the understanding of gonadogenesis process.

Comparison of the Food Quality of Freshwater Rainbow Trout Oncorhynchus mykiss Cultured in Different Regions (생산 지역을 달리한 담수산 무지개송어(Oncorhynchus mykiss)의 식품학적 품질 특성 비교)

  • Kang, Sang In;Kim, Ki Hyun;Lee, Jun Kyu;Kim, Yong Jung;Park, Su-Jin;Kim, Min Woo;Choi, Byeong Dae;Kim, Dongsoo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.2
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    • pp.103-113
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    • 2014
  • This study compared the food quality of freshwater rainbow trout Oncorhynchus mykiss cultured (in Pyeongchang, Pyeongtaek, Jecheon and Geochang) with that of imported salmon Oncorhynchus keta. The proximate composition of the four types of rainbow trout was 74.4-75.7% moisture, 18.3-19.4% crude protein, 3.1-4.3% crude lipid, and 1.3-1.7% ash. There were slight differences in the proximate composition of the four types of rainbow trout. No significant (P>0.05) differences were found in the red color and odor and taste intensities of the four types of rainbow trout based on the Hunter a values, volatile basic nitrogen content, odor intensity using an electronic nose and taste intensity using an electronic tongue. The crude protein, total amino acid, and mineral contents of rainbow trout cultured in Pyeongtaek were superior or similar to those of the trout cultured in the other three places. The rainbow trout cultured in Geochang had the highest hardness, at 858.5 g.

Effect of an Astaxanthin-supplemented Diet on the Nutritional Composition of Rainbow Trout (Oncorhynchus mykiss) (무지개 송어(Oncorhynchus mykiss)의 영양 성분에 대한 astaxanthin 첨가사료의 영향)

  • Choi, Chi-Song;Eom, Sung-Hwan;Lee, Myung-Suk;Kim, Young-Mog
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.2
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    • pp.109-116
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    • 2010
  • This study investigated the effects of astaxanthin, which is used to improve the muscle color of fish, on the nutritional composition of rainbow trout (Oncorhynchus mykiss). The trout were fed extruded pellets containing astaxanthin (pigmented rainbow trout) or without astaxanthin (non-pigmented rainbow trout). No significant differences in the contents of crude protein and crude ash between the two muscles of the two groups were observed. However, the crude fat composition of the muscle of pigmented rainbow trout was about two times higher than that of control muscle. In the muscle of pigmented rainbow trout, the contents of docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3), which are functional polyunsaturated fatty acids, were 1.6 fold higher than in non-pigmented muscle. In addition, the contents of minerals such as Zn and Fe, vitamins (B group, C, and E), free amino acids, carotenoids and astaxanthin were increased in the muscle of rainbow trout fed pellets supplemented with astaxanthin. Specifically, the content of the bioactive compound astaxanthin, was increased six times in the pigmented muscle, as compared to the control muscle. The results suggest that a diet supplemented with astaxanthin improves the nutritional composition of rainbow trout muscle as well as improving the muscle color.

Effect of Lipoxygenase on the Oxidation of Rainbow Trout Lipid in Model system (모델시스템에 있어서 무지개 송어 지방질의 산화에 대한 Lipoxygenase의 영향)

  • 김혜경;엄수현;최홍식
    • Journal of Life Science
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    • v.5 no.2
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    • pp.70-75
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    • 1995
  • The effect of lipoxygenase (LOX) on the oxidation and co-oxidation of lipid fraction was studied in the model system of rainbow trout. For the reaction in model system 1 g of lipid fraction and 50mL of enzyme extract(LOX, 140 unit in 50mL phosphate buffer solution at pH 7, 4)), which were obtained from rainbow trout, were homoginized in the presence of Tween 20 and kept at 23$\circ$C for 3 days. The activity of LOX was decreased to 43% of initial level during the reaction in the model system. The initial composition of rainbow trout lipid was showed to be consisted of trigliceride(TG;82%) and free fatty acid(FFA;0.1%), while this converted to 59% of TG and 20% of FIFA, respectively after reaction in model system. Change of fatty acid composition was also observed and the content of linoleic acid, one of the major fatte acids, was decreased to 13% from 54% in the content of total fatty acids after reaction. The carotenoids in rainbow trout were composed of 0.4% $\alpha$-carotene, 1.6% $\beta$ -carotene, 80% canthaxanthin, 7% lutein and 11% zeaxanthin, thus the canthaxanthin was the major component. This canthaxanthin was the most degraded carotenoid by lipoxygenase catalyzed co-oxidation during the reaction. On the other hand the tocopherol isomers found in the rainbow trout were $\alpha$ and $\beta$ -tocopherol, and $\alpha$-tocopherol had a higher degradation rate by the lipoxygenase catalyzed co-oxidation than of $\beta$-tocopherol in the reaction of model system.

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Determination of Astaxanthin, $\alpha$-Tocopherol and TBARS in the Liver and Muscle of Rainbow Trout Supplemented with Red Yeast Containing Astaxanthin (Astaxanthin을 포함한 Red Yeast를 급여한 무지개 송어 간과 근육의 Astaxanthin, $\alpha$-Tocopherol 및 지질과산화물 함량)

  • 김해리;강지원
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.5
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    • pp.935-939
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    • 1998
  • The concentrations of astaxanthin and $\alpha$-tocopherol were measured from the muscle of the rainbow trout(Oncorhynchus mykiss) that had been fed the red yeast(Phaffia rhodozyma) containing 0.2% astaxanthin for 7, 14 and 21 days. The effect of the astaxanthin supplementation for 21 days on peroxidation of liver and muscle lipids of the rainbow trouts was examined. The astaxanthin was found to be accumulated in the rainbow trout muscle when fed for 7 days with astaxanthin supplementation(80mg/kg diet) in the form of the red yeast and the content did not increase further when fed longer up to 21 days. Seven days supplementation of astaxanthin raised the rainbow trout muscle content of the astaxanthin to 17.3$\mu\textrm{g}$/g tissue from 11.8$\mu\textrm{g}$/g tissue in mature control group. Although the hepatic TBARS level was found to be significantly decreased, the astaxanthin supplementation did not alter the $\alpha$-tocopherol and TBARS contents of the rainbow trout muscle.

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Susceptibility of rainbow trout Oncorhynchus mykiss and cherry salmon Oncorhynchus masou against Ichthyophthirius multifiliis (무지개송어와 산천어의 백점충에 대한 감수성)

  • Kim, Yi-Cheong;Kim, Jin-Do;Jee, Bo-Young;Jung, Sung-Hee;Seo, Jung-Soo;Park, Sung-Woo
    • Journal of fish pathology
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    • v.22 no.3
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    • pp.193-200
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    • 2009
  • This study was performed to natural infection of Ichthyophthirius multifiliis and mortalities of two fish species were compared at 10${^{\circ}C}$ and 17${^{\circ}C}$ after artificial infection with the parasite. Subsequently compared to motality by the artificial infection with the different parasite orgin of host at 17${^{\circ}C}$ using rainbow trout Oncorhynchus mykiss. The prevalence of Ichthyophthirius multifiliis in cherry salmon was higher than that in rainbow trout during the periods of low temperature but no difference in high temperature season. The parasite was not detectable in culturing water and detritus except May that of density has been high. Susceptibility to the parasite was higher in cherry salmon than in rainbow trout at 10${^{\circ}C}$ of water temperature but no difference between two fish species at 17${^{\circ}C}$. When rainbow trout were infected with Ichthyophthirius multifiliis from rainbow trout and cherry salmon at 17${^{\circ}C}$, there was no difference in death of host. These results suggest that cherry salmon have higher susceptibility to Ichthyophthirius multifiliis than rainbow trout at low temperature.

Monitoring of Viral Hemorrhagic Septicemia Virus in Seawater-Reared Rainbow Trout Oncorhynchus mykiss (해수 사육 무지개송어(Oncorhynchus mykiss)의 Viral Hemorrhagic Septicemia Virus (VHSV) 모니터링)

  • Kim, Wi-Sik;Kong, Kyoung-hui;Jeon, Young-Ho;Oh, Myung-Joo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.5
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    • pp.621-623
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    • 2017
  • Mariculture of rainbow trout Onchorhynchus mykiss has been initiated in or around olive flounder Paralichthys olivaceus farms, where viral hemorrhagic septicemia virus (VHSV) is often detected in some fish. In the present study, we investigated VHSV infection in seawater-reared rainbow trout because VHSV has never been detected in salmonids in Korea. A total of 104 adult fish were tested for the presence of VHSV by reverse transcription-polymerase chain reaction, followed by virus isolation with the fathead minnow caudal trunk cell line. Cytopathic effects were observed in two samples but the virus was identified as infectious hematopoietic necrosis virus. Thus, VHSV was not isolated from seawater-reared rainbow trout.

The Novel Angiotensin I Converting Enzyme Inhibitory Peptide from Rainbow Trout Muscle Hydrolysate

  • Kim, Sung-Rae;Byun, Hee-Guk
    • Fisheries and Aquatic Sciences
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    • v.15 no.3
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    • pp.183-190
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    • 2012
  • The purpose of this study was the purification and characterization of an angiotensin I converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of rainbow trout Oncorhynchus mykiss muscle. After removal of lipid, the approximate composition analysis of the rainbow trout revealed 24.4%, 1.7%, and 68.3% for protein, lipid, and moisture, respectively. Among six hydrolysates, the peptic hydrolysate exhibited the highest ACE inhibitory activity. We attempted to purify ACE inhibitory peptides from peptic hydrolysate using high performance liquid chromatography on an ODS column. The $IC_{50}$ value of purified ACE inhibitory peptide was $63.9{\mu}M$. The amino acid sequence of the peptide was identified as Lys-Val-Asn-Gly-Pro-Ala-Met-Ser-Pro-Asn-Ala-Asn, with a molecular weight of 1,220 Da, and the Lineweaver-Burk plots suggested that they act as a competitive inhibitor against ACE. Our study suggested that novel ACE inhibitory peptides purified from rainbow trout muscle protein may be beneficial as anti-hypertension compounds in functional foods.

Experimental Infection of Rainbow Trout (Oncorhynchus mykiss) with Viral Hemorrhagic Septicemia Virus (VHSV, Genotype IVa) from Olive Flounder (Paralichtys olivaceus) (넙치 (Paralichtys olivaceus) 유래 viral hemorrhagic septicemia virus (VHSV, genotype IVa)의 무지개송어 (Oncorhynchus mykiss) 감염 실험)

  • Kim, Wi-Sik;Jeong, Ha-Na;Kong, Kyoung-hui;Kim, Ah-ra;Jeon, Young-Ho;Oh, Myung-Joo
    • Korean Journal of Ichthyology
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    • v.28 no.3
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    • pp.141-146
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    • 2016
  • Experimental infection of rainbow trout Oncorhynchus mykiss with viral hemorrhagic septicemia virus (VHSV, genotype IVa) from olive flounder Paralichtys olivaceus was examined. The cumulative mortalities of three different lot of rainbow trout fry challenged with VHSV ($10^{6.3-7.3}TCID_{50}$/fish) were less than 15%. No difference of virulence was observed in experimental infection using 5 in vivo passaged VHSV and original VHSV. No mortality was observed in seawater-reared rainbow trout (adult) challenged with VHSV ($10^{5.3-6.3}TCID_{50}$/fish) and virus was not detected in the fish. We thus concluded that VHSV from olive flounder has low virulence to rainbow trout fry, but not pathogenic to seawater-rainbow trout (adult).