Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Analysis of Differential Expression of Cytochrome P450 Aromatase(Cyp19) in The Efferent Ductules and The Epididymis of Male Rats During Postnatal Development

생후 발달과정 동안 숫 흰쥐의 Efferent Ductules과 부정소에서 Cytochrome P450 Aromatase(Cyp19) 발현 양상 분석

  • Kim, Ju-Young (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Seo, Hee-Jung (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Kim, Ok-Soon (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Kim, Byung-Joon (Departments of Biochemistry Internal Medicine, Eulji University School of Medicine) ;
  • Lee, Seong-Kyu (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Baik, Haing-Woon (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine) ;
  • Lee, Ki-Ho (Departments of Biochemistry and Molecular Biology and Medical Sciences Research Institute, Eulji University School of Medicine)
  • 김주영 (을지대학교 생화학－분자생물학교실 및 의과학연구소) ;
  • 서희정 (을지대학교 생화학－분자생물학교실 및 의과학연구소) ;
  • 김옥순 (을지대학교 생화학－분자생물학교실 및 의과학연구소) ;
  • 김병준 (을지대학교 생화학－내과학교실) ;
  • 이성규 (을지대학교 생화학－분자생물학교실 및 의과학연구소) ;
  • 백행운 (을지대학교 생화학－분자생물학교실 및 의과학연구소) ;
  • 이기호 (을지대학교 생화학－분자생물학교실 및 의과학연구소)
  • Published : 2008.12.31
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Abstract

The present study was performed to determine expression of cytochrome P450 aromatase(Cyp19) in the efferent ductules(EDs) and the epididymis of male rat reproductive tract at different postnatal ages. Total RNAs isolated were reverse-transcribed, and cDNAs were utilized for real-time PCR analysis. In the EDs, the Cyp19 transcript was expressed at all prepubertal ages with the highest level at 14 days of age, but not at 90 days of age. Expression of Cyp19 mRNA in the epididymis was found at all age groups, except 7 days of age. Distinct expression patterns of Cyp19 transcript were shown in each segment of the epididymis. These results indicate that expression of Cyp19 gene in the excurrent duct of male reproductive tract is differentially regulated in age-dependent and segment-specific manners.

본 연구는 생후 발달과정에서 숫 흰쥐의 생식기인 Efferent ductules(EDs)과 부정소에서 cytochrome P450 aromatase(Cyp19) 유전자의 발현과 연령별 발현 양상을 알아보는데 목적이 있다. 조직으로부터 추출된 total RNA는 역 전사 반응을 통해 cDNA로 바뀌어진 후 real-time PCR 기법을 사용하여 부위별 그리고 연령별로 Cyp19 유전자 발현을 알아보았다. Cyp19 유전자의 발현은 EDs에서 90일령을 제외한 7일, 14일, 그리고 30일령 등 성숙기 이전의 모든 연령에서 나타났으며, 부정소에서는 7일령을 제외한 모든 연령에서 보여졌다. 특히 부정소에서 Cyp19의 발현은 부정소의 각 부위별로 특이한 양상을 보였다. 본 연구 결과를 통해 남성 생식기의 EDs와 부정소의 각 부분에서 Cyp19 유전자 발현은 연령과 부위에 따라 차별적으로 조절되는 것을 알 수 있었다.

Keywords

References

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