Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Effects of the Mechanical Stretch on Aligned Multi-Layered Nanofibrous Scaffolds Seeded with Smooth Muscle Cells

기계적 자극이 다층 구조의 나노파이버 지지체의 평활근 세포에 미치는 영향

  • Shin, Ji-Won (Department of Biomedical Engineering, Inje University) ;
  • Kim, Dong-Hwa (Department of Biomedical Engineering, Inje University) ;
  • Heo, Su-Jin (Department of Biomedical Engineering, Inje University) ;
  • Kim, Su-Hyang (Team of BK21, Inje University) ;
  • Kim, Young-Jick (Department of Dental Laboratory Science, College of Health Science, Catholic University) ;
  • Shin, Jung-Woog (Department of Biomedical Engineering, Inje University)
  • 신지원 (인제대학교 의용공학과) ;
  • 김동화 (인제대학교 의용공학과) ;
  • 허수진 (인제대학교 의용공학과) ;
  • 김수향 (인제대학교 BK21 사업단) ;
  • 김영직 (부산 가톨릭 대학교 치기공학과) ;
  • 신정욱 (인제대학교 의용공학과)
  • Published : 2008.02.29
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Abstract

The object of this study is to investigate the effects of intermittent cyclic stretching on the smooth muscle cells (SMCs) seeded onto aligned multi-layered fibrous scaffold. To make multi-layered fibrous scaffold, polyurethane (PU) and poly(ethylene oxide) (PEO) were electrospun alternatively, then were immersed into distilled water to extract PEO. Various types of scaffolds were fabricated depending on fiber directions, i.e., aligned or randomly oriented. The direction of stretching was either parallel or vertical to the fiber direction for the aligned scaffolds. The stretching was also applied to the randomly aligned scaffolds. The duration of stretching was 2 min with 15 min resting period. During the stretching, the maximum and minimum strain was adjusted to be 10 and 7%, respectively with the frequency of 1 Hz. The bioactivities of cells on the scaffolds were assessed by quantifying DNA, collagen, and glycosaminoglycan (GAG) levels. And the cell morphology was observed by staining F-actin. SMCs under parallel stretching to the fiber direction responded more positively than those in other conditions. From the results, we could explain the morphological effect of a substrate on cellular activities. In addition the synergistic effects of substrate and mechanical stimuli effects were confirmed.

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References

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