Toxicological Research

  • 제24권1호
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  • Pages.29-36
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  • 2008
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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The Effects of Crinum asiaticum on the Apoptosis Induction and the Reversal of Multidrug Resistance in HL-60/MX2

  • Hyun, Jae-Hee (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Kang, Jung-Il (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Kim, Sang-Cheol (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Kim, Elvira (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Kang, Ji-Hoon (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Kwon, Jung-Mi (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Park, Doek-Bae (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Lee, Young-Jae (Department of Veterinary Medicine, College of Applied Life Science, Cheju National University) ;
  • Yoo, Eun-Sook (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University) ;
  • Kang, Hee-Kyoung (Department of Medicine, College of Medicine, College of Applied Life Science, Cheju National University)
  • 발행 : 2008.03.31
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초록

The present study investigated the anti-proliferative and chemosensitizing effects of Crinum asiaticum var. japonicum against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform ($CHCl_3$) fraction and butanol (BuOH) fraction of C. asiaticum inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the $CHCl_3$ and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced BcI-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The $CHCl_3$ fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the $CHCl_3$ fraction of C. asiaticum plays a pivotal role as a chemosensitizer. We suggest that components of C. asiaticum might have a therapeutic potential for the treatment of MDR leukemia.

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참고문헌

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