Journal of Microbiology and Biotechnology

  • 제18권11호
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  • Pages.1858-1861
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  • 2008
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Rapid and Sensitive Detection of Listeria monocytogenes Using a PCR-Enzyme-Linked Immunosorbent Assay

  • Kim, Hye-Jin (Institute of Environmental Science and Engineering, Department of Environmental Sciences, Hankuk University of Foreign Studies) ;
  • Cho, Jae-Chang (Institute of Environmental Science and Engineering, Department of Environmental Sciences, Hankuk University of Foreign Studies)
  • 발행 : 2008.11.30
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초록

A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for the rapid and sensitive detection of L. monocytogenes. PCR primers generating a 132-bp amplicon and a capture probe able to hybridize to the PCR amplicon were designed based on the L. monocytogenes-specific hly gene encoding listeriolysin. The detection limit of PCR-ELISA for L. monocytogenes was determined to be as low as 10 cells per PCR reaction, and this level of detection was achieved within 5 h. These results indicate that the PCR-ELISA provides a valuable tool for the rapid and sensitive detection of L. monocytogenes for the ready-to-eat food industry.

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참고문헌

  1. Arimi, S. M., E. T. Ryser, T. J. Pritchard, and C. W. Donnelly. 1997. Diversity of Listeria ribotypes recovered from dairy cattle, silage, and dairy processing environments. J. Food Prot. 60: 811-816 https://doi.org/10.4315/0362-028X-60.7.811
  2. Autio, T., S. Hielm, M. Miettinen, A. M. Sjoberg, K. Aarnisalo, J. Bjorkroth, T. Mattila-Sandholm, and H. Korkeala. 1999. Sources of Listeria monocytogenes contamination in a cold-smoked rainbow trout processing plant detected by pulsed-field gel electrophoresis typing. Appl. Environ. Microbiol. 65: 150-155
  3. Ben Embarek, P. K. 1994. Presence, detection and growth of Listeria monocytogenes in seafoods: A review. Int. J. Food Microbiol. 23: 17-34 https://doi.org/10.1016/0168-1605(94)90219-4
  4. Bolton, F. J., A. D. Sails, A. J. Fox, D. R. Wareing, and D. L. Greenway. 2002. Detection of Campylobacter jejuni and Campylobacter coli in foods by enrichment culture and polymerase chain reaction enzyme-linked immunosorbent assay. J. Food Prot. 65: 760-767 https://doi.org/10.4315/0362-028X-65.5.760
  5. Bula, C. J., J. Bille, and M. P. Glauser. 1995. An epidemic of foodborne listeriosis in western Switzerland: Description of 57 cases involving adults. Clin. Infect. Dis. 20: 66-72 https://doi.org/10.1093/clinids/20.1.66
  6. Carlin, F., V. Broussolle, S. Perelle, S. Litman, and P. Fach. 2004. Prevalence of Clostridium botulinum in food raw materials used in REPFEDs manufactured in France. Int. J. Food Microbiol. 91: 141-145 https://doi.org/10.1016/S0168-1605(03)00371-4
  7. Daly, P., A. Corcoran, B. P. Mahon, and S. Doyle. 2002. Highsensitivity PCR detection of parvovirus B19 in plasma. J. Clin. Microbiol. 40: 1958-1962 https://doi.org/10.1128/JCM.40.6.1958-1962.2002
  8. Dykes, G. A. and M. Dworaczek. 2002. Influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in a defined medium. Lett. Appl. Microbiol. 35: 538-542 https://doi.org/10.1046/j.1472-765X.2002.01237.x
  9. Farber, J. M. and P. I. Peterkin. 1991. Listeria monocytogenes, a food-borne pathogen. Microbiol. Rev. 55: 476-511
  10. Gallagher, L., E. D. Ebel, and J. R. Kause. 2003. Draft FSIS risk assessment for Listeria in ready-to-eat meat and poultry products. Food Safety and Inspection Service, Washington DC
  11. Gasanov, U., D. Hughes, and P. M. Hansbro. 2005. Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: A review. FEMS Microbiol. Rev. 29: 851-875 https://doi.org/10.1016/j.femsre.2004.12.002
  12. Ge, B., S. Zhao, R. Hall, and J. Meng. 2002. A PCR-ELISA for detecting Shiga toxin-producing Escherichia coli. Microbes Infect. 4: 285-290 https://doi.org/10.1016/S1286-4579(02)01540-X
  13. Goulet, V., J. Rocourt, I. Rebiere, C. Jacquet, C. Moyse, P. Dehaumont, G. Salvat, and P. Veit. 1998. Listeriosis outbreak associated with the consumption of rillettes in France in 1993. J. Infect. Dis. 177: 155-160 https://doi.org/10.1086/513814
  14. Gutierrez, R., T. Garcia, I. Gonzalez, B. Sanz, P. E. Hernandez, and R. Martin. 1998. Quantitative detection of meat spoilage bacteria by using the polymerase chain reaction (PCR) and an enzyme linked immunosorbent assay (ELISA). Lett. Appl. Microbiol. 26: 372-376 https://doi.org/10.1046/j.1472-765X.1998.00352.x
  15. Hong, Y., M. E. Berrang, T. Liu, C. L. Hofacre, S. Sanchez, L. Wang, and J. J. Maurer. 2003. Rapid detection of Campylobacter coli, C. jejuni, and Salmonella enterica on poultry carcasses by using PCR-enzyme-linked immunosorbent assay. Appl. Environ. Microbiol. 69: 3492-3499 https://doi.org/10.1128/AEM.69.6.3492-3499.2003
  16. Jorgensen, L. V. and H. H. Huss. 1998. Prevalence and growth of Listeria monocytogenes in naturally contaminated seafood. Int. J. Food Microbiol. 42: 127-131 https://doi.org/10.1016/S0168-1605(98)00071-3
  17. Jung, S.-J., H.-J. Kim, and H.-Y. Kim. 2005. Quantitative detection of Salmonella typhimurium contamination in milk using realtime PCR. J. Microbiol. Biotechnol. 15: 1353-1358
  18. Kim, B., H. Jeong, S. Baek, J. Shin, J. Kim, K. Min, et al. 2005. Validation of one-step real-time RT-PCR assay in combination with automated RNA extraction for rapid detection and quantitation of hepatitis C virus RNA for routine testing in clinical specimens. J. Microbiol. Biotechnol. 15: 595-602
  19. Kim, H., G. Seo, K. Jung, S. Kim, J. Kim, K. Oh, B. Koo, and Y. Chai. 2007. Generation of a specific marker to discriminate Bacillus anthracis from other bacteria of the Bacillus cereus group. J. Microbiol. Biotechnol. 17: 806-811
  20. Kuhn, M. and W. Goebel. 1999. Pathogenesis of Listeria monocytogenes. Marcel Dekker, Inc., New York, N.Y
  21. Lawrence, L. M. and A. Gilmour. 1995. Characterization of Listeria monocytogenes isolated from poultry products and from the poultryprocessing environment by random amplification of polymorphic DNA and multilocus enzyme electrophoresis. Appl. Environ. Microbiol. 61: 2139-2144
  22. Lawson, A. J., J. M. Logan, L. O'Neill, G. M. Desai, and J. Stanley. 1999. Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay. J. Clin. Microbiol. 37: 3860-3864
  23. Lazar, J. G. 1995. Immunological Detection of PCR Products. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y
  24. Lyytikainen, O., T. Autio, R. Maijala, P. Ruutu, T. Honkanen-Buzalski, M. Miettinen, et al. 2000. An outbreak of Listeria monocytogenes serotype 3a infections from butter in Finland. J. Infect. Dis. 181: 1838-1841 https://doi.org/10.1086/315453
  25. Mengaud, J., M. F. Vicente, J. Chenevert, J. M. Pereira, C. Geoffroy, B. Gicquel-Sanzey, F. Baquero, J. C. Perez-Diaz, and P. Cossart. 1988. Expression in Escherichia coli and sequence analysis of the listeriolysin O determinant of Listeria monocytogenes. Infect. Immun. 56: 766-772
  26. Metzger-Boddien, C., A. Bostel, and J. Kehle. 2004. AnDiaTec Salmonella sp. PCR-ELISA for analysis of food samples. J. Food. Prot. 67: 1585-1590 https://doi.org/10.4315/0362-028X-67.8.1585
  27. Metzger-Boddien, C., D. Khaschabi, M. Schonbauer, S. Boddien, T. Schlederer, and J. Kehle. 2006. Automated high-throughput immunomagnetic separation-PCR for detection of Mycobacterium avium subsp. paratuberculosis in bovine milk. Int. J. Food Microbiol. 110: 201-208 https://doi.org/10.1016/j.ijfoodmicro.2006.01.042
  28. Nesbakken, T., G. Kapperud, and D. A. Caugant. 1996. Pathways of Listeria monocytogenes contamination in the meat processing industry. Int. J. Food Microbiol. 31: 161-171 https://doi.org/10.1016/0168-1605(96)00978-6
  29. O'Sullivan, N. A., R. Fallon, C. Carroll, T. Smith, and M. Maher. 2000. Detection and differentiation of Campylobacter jejuni and Campylobacter coli in broiler chicken samples using a PCR/DNA probe membrane based colorimetric detection assay. Mol. Cell. Probes 14: 7-16 https://doi.org/10.1006/mcpr.1999.0274
  30. Ojeniyi, B., H. C. Wegener, N. E. Jensen, and M. Bisgaard. 1996. Listeria monocytogenes in poultry and poultry products: Epidemiological investigations in seven Danish abattoirs. J. Appl. Bacteriol. 80: 395-401 https://doi.org/10.1111/j.1365-2672.1996.tb03234.x
  31. Perelle, S., F. Dilasser, B. Malorny, J. Grout, J. Hoorfar, and P. Fach. 2004. Comparison of PCR-ELISA and LightCycler realtime PCR assays for detecting Salmonella spp. in milk and meat samples. Mol. Cell. Probes 18: 409-420 https://doi.org/10.1016/j.mcp.2004.07.001
  32. Rorvik, L. M., D. A. Caugant, and M. Yndestad. 1995. Contamination pattern of Listeria monocytogenes and other Listeria spp. in a salmon slaughterhouse and smoked salmon processing plant. Int. J. Food Microbiol. 25: 19-27 https://doi.org/10.1016/0168-1605(94)00080-P
  33. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
  34. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  35. Waters, S. M., S. Doyle, R. A. Murphy, and R. F. Power. 2005. Development of solution phase hybridisation PCR-ELISA for the detection and quantification of Enterococcus faecalis and Pediococcus pentosaceus in Nurmi-type cultures. J. Microbiol. Methods 63: 264-275 https://doi.org/10.1016/j.mimet.2005.03.016
  36. Winters, D. K. and M. F. Slavik. 1995. Evaluation of a PCR based assay for specific detection of Campylobacter jejuni in chicken washes. Mol. Cell. Probes 9: 307-310 https://doi.org/10.1016/S0890-8508(95)91556-7

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