Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Rapid and Sensitive Detection of Listeria monocytogenes Using a PCR-Enzyme-Linked Immunosorbent Assay

  • Kim, Hye-Jin (Institute of Environmental Science and Engineering, Department of Environmental Sciences, Hankuk University of Foreign Studies) ;
  • Cho, Jae-Chang (Institute of Environmental Science and Engineering, Department of Environmental Sciences, Hankuk University of Foreign Studies)
  • Published : 2008.11.30
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Abstract

A PCR-enzyme-linked immunosorbent assay (PCR-ELISA) was developed for the rapid and sensitive detection of L. monocytogenes. PCR primers generating a 132-bp amplicon and a capture probe able to hybridize to the PCR amplicon were designed based on the L. monocytogenes-specific hly gene encoding listeriolysin. The detection limit of PCR-ELISA for L. monocytogenes was determined to be as low as 10 cells per PCR reaction, and this level of detection was achieved within 5 h. These results indicate that the PCR-ELISA provides a valuable tool for the rapid and sensitive detection of L. monocytogenes for the ready-to-eat food industry.

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References

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