Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Sulfide Removal on Sulfate Reduction at pH 5 in a Hydrogen Fed Gas-Lift Bioreactor

  • Bijmans, Martijn F.M. (Sub Department of Environmental Technology, Wageningen University and Research Centre) ;
  • Dopson, Mark (Department of Molecular Biology, Umea University) ;
  • Ennin, Frederick (Sub Department of Environmental Technology, Wageningen University and Research Centre) ;
  • Lens, Piet N.L. (Sub Department of Environmental Technology, Wageningen University and Research Centre) ;
  • Buisman, Cees J.N. (Sub Department of Environmental Technology, Wageningen University and Research Centre)
  • Published : 2008.11.30
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Abstract

Biotechnological treatment of sulfate- and metal-ions-containing acidic wastewaters from mining and metallurgical activities utilizes sulfate-reducing bacteria to produce sulfide that can subsequently precipitate metal ions. Reducing sulfate at a low pH has several advantages above neutrophilic sulfate reduction. This study describes the effect of sulfide removal on the reactor performance and microbial community in a high-rate sulfidogenic gas-lift bioreactor fed with hydrogen at a controlled internal pH of 5. Under sulfide removal conditions, 99% of the sulfate was converted at a hydraulic retention time of 24 h, reaching a volumetric activity as high as 51 mmol sulfate/l/d. Under nonsulfide removal conditions, <25% of the sulfate was converted at a hydraulic retention time of 24 h reaching volumetric activities of <13 mmol sulfate/l/d. The absence of sulfide removal at a hydraulic retention time of 24 h resulted in an average $H_2S$ concentration of 18.2 mM (584 mg S/I). The incomplete sulfate removal was probably due to sulfide inhibition. Molecular phylogenetic analysis identified 11 separate 16S rRNA bands under sulfide stripping conditions, whereas under nonsulfide removal conditions only 4 separate 16S rRNA bands were found. This shows that a less diverse population was found in the presence of a high sulfide concentration.

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References

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