한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제36권3호
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  • Pages.189-194
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  • 2008
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

사상설 진균 Aspergillus nidulans의 Phospholipase B 유전자(plb A)의 클로링

Molecular Cloning of a Putative Gene Encoding Phospholipase B (plbA) from Aspergillus nidulans

  • 홍사현 (국립보건연구원 감염병센터 장내세균팀) ;
  • 조은민 (한국기초과학지원연구원 서울센터 메타볼륨분석연구팀) ;
  • 송승은 (한국기초과학지원연구원 서울센터 메타볼륨분석연구팀) ;
  • 엄치용 (한국기초과학지원연구원 서울센터 메타볼륨분석연구팀)
  • Hong, Sa-Hyun (Division of Enteric Bacterial Infections, Center for Infectious Diseases, Korean National Institute of Health) ;
  • Cho, Eun-Min (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute) ;
  • Song, Seung-Eun (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute) ;
  • Eom, Chi-Yong (Metabolome Analysis Team, Seoul Center, Korea Basic Science Institute)
  • 발행 : 2008.09.28
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초록

Phospholipase B(PLB) families는 phospholipase(PL), lysophospholipase(LPL) 그리고 lysophospholipase-transacylase(LHA)의 활성을 공유하고 있는 효소이다. 본 연구에서는, 사상성 진균인 Aspergillus nidulans에서 최초로 lipase motifs를 보유하고 있는 단백질 Phospholipase B를 인코딩하는 유전자(plb A)를 클로닝하였다. plb A는 다양한 PLB 효소들의 lipase 보존영역들의 염기서열 정보에 근거하여 제작한 probe를 이용하여 A. nidulans genomic DNA library로 부터 분리하였다. Phospholipase B 유전자의 염기서열을 결정한 결과 626아미노산으로 구성된 단백질을 코드하고 있었다. PlbA는 Penicillium notatum의 PLB와는 72%의 높은 상동성을 나타내었으나, 다른 생물유래의 PLA와는 낮은 상동성을 나타내었다.

The phospholipase B (PLB) families are enzymes sharing phospholipase (PL), lysophospholipase (LPL) and lysophospholipase-transacylase (LPTA) activities. In this study, we report the putative gene encoding phospholipase B (plbA) containing lipase motifs was cloned for the first time from the filamentous fungus, Aspergillus nidulans. plbA was isolated from A. nidulans genomic DNA library using a PCR-amplified probe, which is designed on the basis of sequence information derived from the conserved lipase regions of various PLBs. The deduced product of plbA is of 626 amino acids. From the assigned sequence, PlbA showed 72% identity with Penicillium notatum PLB but have low similarity with phospholipase A of other organisms.

키워드

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