한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제36권4호
  • /
  • Pages.291-298
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  • 2008
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  • 1598-642X(pISSN)
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  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

적포도주들의 발효와 후 발효 중 심혈관 관련 Angiotensin I 전환효소 저해활성과 혈전용해활성 및 $\beta$-secretase 저해 활성의 변화

Changes of Angiotensin I-Converting Enzyme Inhibitory Activity, Fibrinolytic Activity and $\beta$-Secretase Inhibitory Activity of Red Wines During Fermentation and Post-Fermentation

  • 노재덕 (배재대학교 생명유전공학과) ;
  • 이은나 (배재대학교 생명유전공학과) ;
  • 서동수 (배재대학교 생명유전공학과) ;
  • 천종필 (충남대학교 원예과) ;
  • 최신양 (한국식품연구원) ;
  • 이종수 (배재대학교 생명유전공학과)
  • No, Jae-Duck (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Lee, Eun-Na (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Seo, Dong-Soo (Department of Life Science and Genetic Engineering, Paichai University) ;
  • Chun, Jong-Pil (Department of Horticulture, Chungnam National University) ;
  • Choi, Shin-Yang (Korea Food Research Institute) ;
  • Lee, Jong-Soo (Department of Life Science and Genetic Engineering, Paichai University)
  • 발행 : 2008.12.28
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초록

본 연구는 4종류의 한국산 포도를 이용하여 포도주를 제조한 후 이들의 발효와 후 발효중의 심혈관 관련 angiotensis I 전환효소 저해 확정과 혈전 용해 활성 및 항치매성 $\beta$-secretase 저해활성을 조사하였다. 발효 10일 후 모든 시료 포도주들의 항고혈압성 엔지오텐신 전환효소(ACE)저해활성은 $38.6%{\sim}58.8%$ 이었다. 그러나 후발효가 진행됨에 따라 ACE저해활성은 증가하여 세리단(Vitis hybrid) 포도주가 후발효 120일 후 최고인 76.9%에 도달하였다. 혈전용해활성은 모든 시료 포도주들에서 미약하거나 없었다. 발효 10일 후, 켐벨어리(Vitis labrusca B) 포도주가 54.8%의 가장 높은 항치매성 $\beta$-secretase저해 활성을 보였으나 후발효 120일 후에는 10% 미만으로 현저하게 감소되었다. 결론적으로 본 연구에서는 세리단 포도를 S. cerevisiae K-7 효모로 $25^{\circ}C$에서 10일간 발효 시킨 후 $4^{\circ}C$에서 120일간 후발효 시켜서 고부가가치의 생리 기능성을 가진 세리단 적포도주를 제조하였다.

The cardiovascular angiotensin I-converting enzyme inhibitory activity, fibrinolytic activity and bbb-secretase inhibitory activity of four kinds of red wine were investigated during fermentation and post-fermentation. After 10 days of fermentation, the antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activities of all the red wines ranged from 38.6% to 58.8%. However, the ACE inhibitory activities increased with the prolongation of the post-fermentation period; moreover, in the Vitis hybrid red wine, the ACE inhibitory activity reached its highest value, 76.9%, after 120 days of post-fermentation. During the fermentation and post-fermentation of all the red wines, fibrinolytic activity was weak or not detected. After 10 days of fermentation, Vitis labrusca B red wine exhibited the greatest antidementia $\beta$-secretase inhibitory activity of 54.8%, though $\beta$-secretase inhibitory activity decreased significantly to less than 10% during 120 days of post-fermentation. In conclusion, we obtained a highly valuable Vitis hybrid red wine that was fermented for 10 days at $25^{\circ}C$ with Vitis hybrid and S. cerevisiae K-7 and then post-fermentation for 120 days at $4^{\circ}C$.

키워드

참고문헌

  1. Amous, A., D. Makris, and P. Kefalas. 2001. Effect of principal polyphenolic components in relation antioxidant characterics of aged red wines. J. Agric. Food Chem. 49: 5736-5742 https://doi.org/10.1021/jf010827s
  2. Ariyoshi, Y. 1993. Angiotensin converting enzyme inhibitors derived from food protein. Trends. Food Sci. Tech. 4: 139-144 https://doi.org/10.1016/0924-2244(93)90033-7
  3. Byun, H. G., Y. T. Kim, P. J. Park, X. Lin, and S. K. Kim. 2005. Chitooligosaccharides as a novel ${\beta}-secretase$ inhibitor. Carbohydr. Polym. 61: 198-202 https://doi.org/10.1016/j.carbpol.2005.05.003
  4. Choi, Y. M., K. W. Yu, N. S. Han, J. H. Koh, and J. S. Lee. 2006. Antioxidant activities and antioxidant compounds of commercial red wines. J. Food Sci. Nutr. 35: 1286-1290 https://doi.org/10.3746/jkfn.2006.35.9.1286
  5. Cushman, D. W. and H. S. Cheung. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20: 1637-1648 https://doi.org/10.1016/0006-2952(71)90292-9
  6. Fayek, K. I. and S. T. El-Sayed. 1980. Purification and properties of fibrinolytic enzyme from Bacillus subtilis. Z. Allg. Mikrobiol. 20: 375-382 https://doi.org/10.1002/jobm.3630200603
  7. Frankel, E. N., J. Kanner, J. B. German, E. Parks, and J. E. Kinsella. 1993. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet. 341: 454-457 https://doi.org/10.1016/0140-6736(93)90206-V
  8. Jayaprakasha, G. K., R. P. Singh, and K. K. Sakariah. 2001. Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidatin models in Vitro. Food chem. 73: 285-290 https://doi.org/10.1016/S0308-8146(00)00298-3
  9. Kallithraka, S., I. Arvanitoyanins, A. El-zajouli, and P. Kefalas. 2001. The application of an improved method for trans-resveratrol to determine the origin of Greek red wines. Food chem. 75: 355-363 https://doi.org/10.1016/S0308-8146(01)00213-8
  10. Kanner, J., E. N. Frankel, R. Granit, B. German, and J. E. Kinsella. 1994. Natural antioxidants in grapes and wines. J. Agric. Food Chem. 42: 64-69 https://doi.org/10.1021/jf00037a010
  11. Kim, J. H., D. H. Lee, S. H. Lee, S. Y. Choi, and J. S. Lee. 2004. Effect of Ganoderma lucidum on the quality and functionality of Korean traditional rice wine, Yakju. J. Biosci. Bioeng. 97: 24-28 https://doi.org/10.1016/S1389-1723(04)70160-7
  12. Kim, H. J., D. H. Lee, Y. Y. Hwang, K. S. Lee, and J. S. Lee. 2005. Characterization of ${\beta}-Hydroxy-{\beta}-methylglutaryl$ Coenzyme A reductase inhibitor from Pueraria thunbergiana. J. Agric. Food Chem. 53: 5882-5888 https://doi.org/10.1021/jf0505978
  13. Kim, J. H., D. H. Lee, S. C. Jeong, K. S. Chung, and J. S. Lee. 2004. Characterization of antihypertensive angiotensin I-converting enzyme inhibitor from Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 14: 1318-1323
  14. Kim, J. H., S. H. Lee, N. M. Kim, S. Y. Choi, J. Y. Yoo, J. S. Lee. 2000. Manufacture and physiological functionality of Korean traditional liquors by using dandelion (Taraxacum platycarpum). Kor. J. Biotechnol. Bioeng. 28: 367-371
  15. Koh, K. H., J. H. Lee, K. R. Yoon, S. Y. Choi, and K. L. Seo 1998. Phenolic compounds of Korean red wine and their superoxide radical scavenging activity. Food Sci. Biotechnol. 7: 131-136
  16. Koh, S. K., J. E. Lee, H. W. Kim, S. S. Kim, Y. K. Park, Y. H. Park, Y. H. Park, and K. H. Koh. 2004. Identification and deacidification of lactic acid bacteria in Korean red wine. Food Sci. Biotechnol. 13: 96-99
  17. Lee, D. H., J. H. Kim, N. M. Kim, J. S. Park, and J. S. Lee. 2002. Manufacture and physiologocal functionality of Korean liquors by using chamomile (Matricaria chamomile). Kor. J. Food Sci. Technol. 34: 109-113
  18. Lee, D. H., J. H. Kim, N. M. Kim, J. S. Park, and J. S. Lee. 2002. Manufacture and physiological functionality of Korean traditional liquors by using Paecilomyeces japonica. Kor. J. Mycol. 30: 142-146 https://doi.org/10.4489/KJM.2002.30.2.142
  19. Lee, D. H., J. H. Kim, J. S. Park, Y. J. Choi, and J. S. Lee. 2004. Isolation and characterization of a novel angiotensin Iconverting enzyme inhibitory peptide derived from the edible mushroom Tricholoma giganteum. Peptides 25: 621-627 https://doi.org/10.1016/j.peptides.2004.01.015
  20. Lee, S. J., J. E. Lee, and S. S. Kim. 2004. Development of Korean red wines using various grape varieties and preference measurement. Kor. J. Food Sci. Technol. 36: 911-918
  21. Lee, S. Y., K. H. Lee, K. S. Chang, and S. K. Lee. 2000. The changes of aroma in wine treated with reverse osmosis system. Kor. J. Food Sci. Technol. 32: 17-24
  22. Lu, Y. amd F. L. Yeap 1999. The polyphenol contituents of grape pomace. Food chem. 65: 1-8 https://doi.org/10.1016/S0308-8146(98)00245-3
  23. Meyer, A., O. Yi, D. Pearson, A. L. Waterhouse, and E. Frankel. 1997. Inhibition of human low density lipoprotein oxidation in relation to phenolic antioxidants in grape(Vitis vinifera). J. Agric. Food Chem. 45: 1638-1643 https://doi.org/10.1021/jf960721a
  24. Moroney, M. A., M. J. Alcaraz, R. A. Forder, F. Carey, and J. R. Hoult. 1988. Selectivity of neutrophil 5-lipoxygenase and cyclo oxygenase inhibition by an anti-inflammatory flavonoid glycoside and related aglycone flavonoids. J. Pharm. Pharmacol. 40: 787-792 https://doi.org/10.1111/j.2042-7158.1988.tb05173.x
  25. No, J. D., D. H. Lee, Y. S. Hwang, S. H. Lee, D. H. Lee, and J. S. Lee. 2008. Change of physicochemical properties and antioxidant activities of red wines during fermentation and post-fermentation. Kor. J. Microbiol. Biotechnol. 30:67-71
  26. Park, S. Y., J. S. Lee, K. W. Yu, N. S. Han, H. Lee, J. H. Koh, and K. S. Shin. 2006. Anti-complementary polysaccharides in grape wines. J. Korean Soc. Food Sci. Nutr. 35: 1232-1236 https://doi.org/10.3746/jkfn.2006.35.9.1232
  27. Renaud, S. and M. de Lorgeril. 1992. Wine, alcohol, platelets, and the French paradox for coronary heart disease. Lancet. 339: 1523-1526 https://doi.org/10.1016/0140-6736(92)91277-F
  28. Rhyu, M. R., Y. J. Nam, and H. Y. Lee. 1996. Screening of angiotensin converting enzyme inhibitors in cereals and legumes. Food Sci. Biotechnol. 5: 334-347
  29. Saito, Y., K. Wanezaki, A. Kawato, and S. Imayasu. 1994. Structure and activity of angiotensin I-converting enzyme inhibitory peptides from sake and sake lees. Biosci. Biotechnol. Biochem. 58: 1767-1771 https://doi.org/10.1271/bbb.58.1767
  30. Sato, M., N. Ramarathnam, Y. Suzuki, T. Ohkudo, M. Takeuchi, and H. Ochi. 1997. Food factors for cancer prevention, superoxide radical scavenging activities of wines, and antioxidative properties of fractions recovered from merlot wine pomace. Springer Press, Tokyo, Japan. pp. 359
  31. Serafini, M, A. Ghiselli, A. Ferro-Luzzi, and C. A. S. Melville. 1994. Red wine, tea, and antioxidants. Lancet. 344: 626 https://doi.org/10.1016/S0140-6736(94)92017-6
  32. Teissedre, P. L., E. N. Frankel, A. L. Waterhouse, H. Peleg, and J. B. German. 1996. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. J. Sci. Food Agric. 70: 55-61 https://doi.org/10.1002/(SICI)1097-0010(199601)70:1<55::AID-JSFA471>3.0.CO;2-X
  33. Tsutomu, T. and K. Yokotsuka. 1999. Angiotensin I-converting enzyme inhibitory peptides from wine. Am. J. Enol. Vitic. 50: 65-68
  34. Yilmaz, Y. and R. T. Toledo. 2006. Oxygen radical absorbance capacities of grape wine industry byproducts and effect of solvent type on extraction of grape seed polyphenols. J. Food Compost. Anal. 19: 41-48 https://doi.org/10.1016/j.jfca.2004.10.009