KSBB Journal

  • 제23권5호
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  • Pages.460-462
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  • 2008
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Epidermal growth factor 발현을 위한 화분립의 이용

Utilization of pollen grains for the expression of epidermal growth factor

  • 최병진 (대구가톨릭대학교 원예학과, 생명공학과) ;
  • 박희성 (대구가톨릭대학교 원예학과, 생명공학과)
  • Choi, Byung-Jin (Department of Horticulture, Department of Biotechnology, Catholic University of Daegu Kyungsan) ;
  • Park, Hee-Sung (Department of Horticulture, Department of Biotechnology, Catholic University of Daegu Kyungsan)
  • 발행 : 2008.10.31
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초록

개화시기의 수술로부터 수집한 백합화분을 aluminum oxide 미세입자와 섞어 교반에 의해 상해를 발생시켰다. 이어서 이들 화분은 signal peptide-fused epidermal growth factor (EGF) DNA를 지니는 Agrobacterium 세포로 vacuum infiltration을 시켰으며 24 hr 화분신장을 통한 배양을 실시하였다. 이들 신장 화분에서의 EGF mRNA 및 단백질 발현은 성공적으로 확인되었으며 이는 cDNA blot hybridization 및 immuno-blotting의 분석결과이다.

Pollens grains collected from fully dehisced lily (Lilium longiflorum) anthers were given wounds by means of shaking in the presence of aluminum oxide particles. They were transformed by infiltration with Agrobacterium cells harboring a synthetic DNA encoding signal peptide-fused epidermal growth factor (EGF). After incubation for 24 hr in vitro, the pollen culture showed that EGF mRNAs and proteins were successfully expressed in the analysis of cDNA blot hybridization and immuno-blotting.

키워드

참고문헌

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