KSBB Journal

  • 제23권5호
  • /
  • Pages.398-402
  • /
  • 2008
  • /
  • 1225-7117(pISSN)
  • /
  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

시아노박테리아의 세포외산물에 대한 연구

Extracellular Products from Cyanobacteria

  • 권종희 (베를린 공과대학 생물공학과) ;
  • 김기은 (서경대학교 생물공학과)
  • Kwon, Jong-Hee (Fachgebiet Technische Bioverfahrenstechnik Institut fur Biotechnologie Technische Universitat Berlin) ;
  • Kim, Gi-Eun (Department of Biotechnology, Seokyeong University)
  • 발행 : 2008.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Cyanobacteria havebeen identified as one of the most promising group producing novel biochemically active natural products. Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Though many useful natural products have been identified in cyanobacterial biomass, cyanobacteria produce also extracellular proteins related with NRPS/PKS. Detection of unknown secondary metabolites in medium was carried in the present study by a screening of 98 cyanobacterial strains. A degenerated PCR technique as molecular approaches was used for general screening of NRPS/PKS gene in cyanobacteria. A putative PKS gene was detected by DKF/DKR primer in 38 strains (38.8%) and PCR amplicons resulted from a presence of NRPS gene were showed by MTF2/MTR2 primer in 30 strains (30.6%) and by A3/A7 primer in 26 strains (26.5%). HPLC analysis for a detection of natural products was performed in extracts from medium in which cyanobacteria containing putative PKS or NRPS were cultivated. CBT57, CBT62, CBT590 and CBT632 strains were screened for a production of extracellular natural products. 5 pure substances were detected from medium of these cyanobacteria.

키워드

참고문헌

  1. Fenical, W. (1993), Chemical Studies of Marine-Bacteria - Developing a New Resource. Chem. Rev. 93, 1673-1683 https://doi.org/10.1021/cr00021a001
  2. Moore, B. S. and J. N. Hopke (2001), Discovery of a new bacterial polyketide biosynthetic pathway. Chembiochem 2, 35-38 https://doi.org/10.1002/1439-7633(20010105)2:1<35::AID-CBIC35>3.0.CO;2-1
  3. Chen, G, G. Y. Wang, X. Li, B. Waters, and J. Davies (2000), Enhanced productionofmicrobial metabolites in the presence of dimethyl sulfoxide. J. Antibiot (Tokyo) 53, 1145-1153 https://doi.org/10.7164/antibiotics.53.1145
  4. Wagner-Dobler, I., W. Beil, S. Lang, M. Meiners, and H. Laatsch (2002), Integrated approach to explore the potential of marine microorganisms for the production of bioactive metabolites. Adv Biochem. Eng. Biotechnol. 74, 207-238 https://doi.org/10.1007/3-540-45736-4_10
  5. Stevens H. (2004), Communities of heterotrophic bacteria in the German Wadden Sea-diversity, dynamics and abundance. Dissertation. Institute for Chemistry and Biology of the Marine Environment, Universität Oldenburg. Oldenburg, Germany
  6. Stachelhaus, T., A. Hüser, and M. A. Marahiel (1996), Biochemical characterization of peptidyl carrier protein (PCP), the thiolation domain of multifunctional peptide synthetases. Chem. Biol. 3, 913-921 https://doi.org/10.1016/S1074-5521(96)90180-5
  7. Marahiel, M. A., T. Stachelhaus, and H. D. Mootz (1997) Modular Peptide Synthetases Involved in Nonribosomal Peptide Synthesis. Chem. Rev. 97, 2651-2673 https://doi.org/10.1021/cr960029e
  8. Kwok, S., S. Y. Chang, J. J. Sninsky, and A. Wang (1994), A guide to the design and use of mismatched and degenerate primers. PCR Methods Appl. 3(4), p.S39-47 https://doi.org/10.1101/gr.3.4.S39
  9. Dittmann, E., B. A. Neilan, M. Erhard, H. von Dohren, and T. Borner (1997), Insertional mutagenesis of a peptide synthtase gene which is responsible for hepatoxin production in the cyanobacterium Microcystis PCC7806. Mol. Microbiol. 26(4), 779-787 https://doi.org/10.1046/j.1365-2958.1997.6131982.x
  10. Schwarzer, D. and M. A. Marahiel (2001), Multimodular biocatalysts for natural product assembly. Naturwissenschaften 88, 93-101 https://doi.org/10.1007/s001140100211
  11. Franche, X. and T. G. Damerval (1988), Tests on nif probes and DNA-Hybridization, Meth. Enzymol. 167, 803-808 https://doi.org/10.1016/0076-6879(88)67091-1