The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Transgenic Rice Plants Expressing an Active Tobacco Mitogen-activated Protein Kinase Kinase Induce Multiple Defense Responses

  • Jeong, Jin-A (Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Yoo, Seung-Jin (Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Yang, Douck-Hee (Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Shin, Seo-Ho (Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA) ;
  • Lee, Myung-Chul (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Cho, Baik-Ho (Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Yang, Kwang-Yeol (Agricultural Plant Stress Research Center, Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University)
  • Published : 2008.12.01
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Abstract

It is well known that NtMEK2, a tobacco MAPK kinase, is the upstream kinase of both salicylic acid-induced protein kinase and wound-induced protein kinase. In addition, expression of $NtMEK2^{DD}$, a constitutively active mutant of NtMEK2, is known to induce multiple defense responses in tobacco. In this study, transgenic rice plants that contained an active or inactive mutant of NtMEK2 under the control of a steroid inducible promoter were generated and used to determine if a similar MAPK cascade is involved in disease resistance in rice. The expression of $NtMEK2^{DD}$ in transgenic rice plants resulted in HR-like cell death. The observed cell death was preceded by the activation of endogenous rice 48-kDa MBP kinase, which is also activated by Xanthomonas oryzae pv. oryzae, the bacterial blight pathogen of rice. In addition, prolonged activation of the MAPK induced the generation of hydrogen peroxide and up-regulated the expression of defense-related genes including the pathogenesis-related genes, peroxidases and glutathione S-transferases. These results demonstrate that NtMEK2 is functionally replaceable with rice MAPK kinase in inducing the activation of the downstream MAPK, which in turn induces multiple defense responses in rice.

Keywords

References

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