Color Doppler Ultrasonographic Evaluation in Observing the Affects of $Osteoset^{(R)}$ on Radial Defects in Beagle Dogs

개에서 $Osteoset^{(R)}$의 요골 결손부 충진에 대한 색도플러초음파검사의 유용성 평가

  • Jeon, Jong-Hoon (College of Veterinary Medicine, Kyungpook National University) ;
  • Seong, Yun-Sang (College of Veterinary Medicine, Kyungpook National University) ;
  • Eom, Ki-Dong (College of Veterinary Medicine, Konkuk University) ;
  • Kim, Jae-Hoon (College of Veterinary Medicine, Cheju National University) ;
  • Jang, Kwang-Ho (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2008.02.29

Abstract

This study is conducted to compare ultrasonographs with plain radiographs in monitoring bone regeneration during reconstruction of critical sized radial defects in dogs. A 15 mm bony defect was taken on each of the eight dog's radius using an electrical saw and an external fixator was applied. The experimental groups were divided into non-treated group(group 1) and $Osteoset^{(R)}$-treated group(group 2). Each fracture site was evaluated using plain radiography and ultrasonography. Radiographic callus formation occurred after $11.50{\pm}1.12$ days in group 1 and $11.50{\pm}0.5$ days in group 2. Neovascularized flow signal could be seen $6.50{\pm}1.5$ days and the vascular signal disappeared after $45.00{\pm}6.16$ days after operation in group 1. Neovascularized flow signal was observed $6.75{\pm}1.78$ days and vascular signal disappeared $23.25{\pm}3.03$ days after surgery which was caused by acoustic shadowing in group 2. Early stages of regeneration were observed more clearly with color Doppler ultrasonography than with plain radiography. Also from the results it is concluded that color Doppler ultrasonography are useful in observing initial stages of bone repair.

Keywords

References

  1. Abiri MM, Kirpekar M, Ablow RC. Osteomyelitis: detection with US. Radiology 1989; 172: 509-511 https://doi.org/10.1148/radiology.172.2.2664872
  2. Ann L. Johnson, Donald A. Hulse. Fundamentals of orthopedic surgery and fracture management. In: Small animal surgery, 2nd ed. St. Louis: Mosby. 2002: 821-900
  3. Brinker WO, Piermattei DL, Flo GL. Fractures: Classification, Diagnosis, and Treatment. In: Handbook of small animal orthopedics and fracture treatment, 2nd ed. Philadelphia: Saunders. 1990: 3-58
  4. Caruso G, Lagalla R, Derchi L, Iovane A, Sanfilippo A. Monitoring of fracture calluses with color Doppler sonography. J Clin Ultrasound 2000; 28: 20-27 https://doi.org/10.1002/(SICI)1097-0096(200001)28:1<20::AID-JCU3>3.0.CO;2-W
  5. Cho BJ, Kim TG, Yang JD. Effect of calcium sulfate-chitosan composite: pellet on bone formation in bone defect. J Craniofacial Surgery 2005; 16: 213-224 https://doi.org/10.1097/00001665-200503000-00006
  6. Claes L, Eckert-Hubner K, Augat P. The fracture gap size influences the local vascularization and tissue differentiation in callus healing. Langenbecks Arch Surg 2003; 388: 316-322 https://doi.org/10.1007/s00423-003-0396-0
  7. Derbyshire ND, Simpson AH. A role for ultrasound in limb lengthening. Br J Radiol 1992; 65: 576-580 https://doi.org/10.1259/0007-1285-65-775-576
  8. Gibbon WW, Long G, Barron DA, O'Connor PJ. Complications of orthopedic implants: sonographic evaluation. J Clin Ultrasound 2002; 30: 288-299 https://doi.org/10.1002/jcu.10065
  9. Hausman MR, Schaffler MB, Majeska RJ. Prevention of fracture healing in rats by an inhibitor of angiogenesis. Bone 2001; 29: 560-564 https://doi.org/10.1016/S8756-3282(01)00608-1
  10. Maffulli N, Hughes T, Fixsen J. Ultrasonographic monitoring of limb lengthening. J Bone Joint Surg Br 1992; 74: 130-132 https://doi.org/10.2106/00004623-199274010-00017
  11. Maffulli N, Thornton A. Ultrasonographic appearance of external callus in long-bone fractures. Injury 1995; 26: 5-12 https://doi.org/10.1016/0020-1383(95)90544-8
  12. Malde HM, Hemmadi SS, Chadda D, Parihar ML, Bhosale PB, Kerdar RP. The role of skeletal sonography in limb lengthening procedures. J Postgrad Med 1993; 39: 127-129
  13. Matsuyama J, Ohnishi I, Kageyama T, Oshida H, Suwabe T, Nakamura K. Osteogenesis and angiogenesis in regeneration bone during transverse distraction. Clin Orthop 2005; 433: 243-250
  14. Mckibbin B. The biology of fracture healing in long bones. J Bone Joint Surg 1978; 60: 150-162
  15. Moed BR, Kim EC, van Holsbeeck M. Ultrasound for the early diagnosis of tibial fracture healing after static interlocked nailing without reaming: histologic correlation using a canine model. J Orthop Trauma 1998; 12: 200-205 https://doi.org/10.1097/00005131-199803000-00012
  16. Moed BR, Subramanian S, van Holsbeeck M. Ultrasound for the early diagnosis of tibial fracture healing after static interlocked nailing without reaming: clinical results. J Orthop Trauma 1998; 12: 206-213 https://doi.org/10.1097/00005131-199803000-00013
  17. Moed BR, Watson JT, Goldschmidt P, Holsbeeck MV. Ultrasound for the early diagnosis fracture healing after interlocking nailing of the tibia without reaming. Clin Orthop 1995; 310: 137-144
  18. Nocini PF, Albanese M, Wangerin K, Fior A, Trevisiol L, Kretschmer W. Distraction osteogenesis of the mandible: evaluation of callus distraction by B-scan ultrasonography. J Craniomaxillofac Surg 2002; 30: 286-291 https://doi.org/10.1016/S1010-5182(02)90307-0
  19. Ohlerth S, Kaser-Hotz B. A review of Doppler sonography for assessment of tumor vascularity. Veterinary and Comparative Oncology 2003; 1: 121-130 https://doi.org/10.1111/j.1476-5829.2003.00020.x
  20. Ricciardi L, Perissinotto A, Dabala M. Mechanical monitoring of fracture healing using ultrasound imaging. Clin Orthop 1993; 293: 71-76
  21. Risselada M, Kramer M, de Rooster H, Taeymans O, Verleyen P, van Bree H. Ultrasonographic and radiographic assessment of uncomplicated secondary fracture healing of long bones in dogs and cats. Vet surg 2005; 34: 99-107 https://doi.org/10.1111/j.1532-950X.2005.00017.x
  22. Risselada M, Kramer M, van Bree H. Approaches for ultrasonographic evaluation of long bones in the dog. Vet Radiol Ultrasound 2003; 44: 214-220 https://doi.org/10.1111/j.1740-8261.2003.tb01274.x
  23. Rodruguez-Merchan EC, Forriol F. Nonunion: general principles and experimental data. Clin Orthop 2004; 419: 4-12 https://doi.org/10.1097/00003086-200402000-00003
  24. Saraf SK. Prediction of fracture healing by ultrasonography and its quantitation by acousto ultrasonic technique-experimental and clinical studies. Indian J Orthop 2001; 35: 2-12
  25. Smith JW, Arnoczky SP, Hersh A. The intraosseous blood supply of the fifth metatarsal: implications for proximal fracture healing. Foot Ankle 1992; 13: 143-152 https://doi.org/10.1177/107110079201300306
  26. Thurmuller P, Troulis M, O'Neill MJ, Kaban LB. Use of ultrasound to assess healing of a mandibular distraction wound. J Oral Maxillofac Surg 2002; 60: 1038-1044 https://doi.org/10.1053/joms.2002.34417
  27. Tselentakis G, Kitano M, Owen PJ, Kuiper JH, Richardson JB, Evans GA. The behaviour of the periosteum during callotasis. J Pediatr Orthop B 2003; 12: 277-283 https://doi.org/10.1097/00009957-200307000-00008
  28. Young JW, Kostrubiak IS, Resnik CS, Paley D. Sonographic evaluation of bone production ar the distraction site in Ilizarov limb-lengthening procedures. AJR 1990; 154: 125-128 https://doi.org/10.2214/ajr.154.1.2104695
  29. 김영민, 정문상, 성상철. 골절학. 서울: 군자출판사. 2001: 25-101