한국물환경학회지 (Journal of Korean Society on Water Environment)

  • 제24권2호
  • /
  • Pages.195-200
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  • 2008
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  • 2289-0971(pISSN)
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  • 2289-098X(eISSN)
한국물환경학회 (Korean Society on Water Environment)
권호 표지

유전자지문분석법(T-RFLP)을 이용한 하천 미생물의 다양성 평가

Evaluation of Riverine Microbial Diversity using the Culture-Independent Genetic Fingerprinting Technique (T-RFLP)

  • 정주용 (경기도보건환경연구원 수질연구부) ;
  • 이경희 (경기도보건환경연구원 수질연구부)
  • Jeong, Ju-Yong (Department of Water Quality Research, Gyeonggido Institute of Health and Environment) ;
  • Lee, Kyong-Hee (Department of Water Quality Research, Gyeonggido Institute of Health and Environment)
  • 투고 : 2008.01.07
  • 심사 : 2008.02.26
  • 발행 : 2008.03.30
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초록

To analyze the riverine microbial community structure, genetic fingerprints and ecological indexes such as species abundances, diversity, evenness, dominance of targeted rivers in Gyeonggi Province were acquired and evaluated using terminal restriction fragment length polymorphism (T-RFLP) technique. Genetic fingerprinting technique such as T-RFLP, which is able to show the microbial community clearly unlike traditional culture-dependent techniques, was thought to be useful to analyse the riverine microbial ecosystem under various factors. Riverine ecosystem evaluation using visible organisms would give biased results with time, targeted organism and researcher. But, T-RFLP, which can exclude the subjected biases such as culture condition and identification, would be an option to understand natural ecosystem by including the microorganisms that defy culture but perform important functions.

키워드

참고문헌

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