Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Identification of a Cryptic Type III Polyketide Synthase (1,3,6,8-Tetrahydroxynaphthalene Synthase) from Streptomyces peucetius ATCC 27952

  • Ghimire, Gopal Prasad (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University) ;
  • Oh, Tae-Jin (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University) ;
  • Liou, Kwangkyoung (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University) ;
  • Sohng, Jae Kyung (Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University)
  • Received : 2008.04.07
  • Accepted : 2008.07.04
  • Published : 2008.10.31
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Abstract

We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydroxynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation

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