Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Plant Regeneration via Secondary Somatic Embryogenesis and Acclimatization in Panax ginseng

장뇌삼의 2차 체세포배 발생을 통한 식물체 유도 및 순화

  • Lee, Su-Gwang (Lab. of Environmental Biotechnology, Department of Environmental & Ecological Engineering, College of Life Science, Dongguk University) ;
  • Kim, Ji-Hee (Lab. of Environmental Biotechnology, Department of Environmental & Ecological Engineering, College of Life Science, Dongguk University) ;
  • Kang, Ho-Duck (Lab. of Environmental Biotechnology, Department of Environmental & Ecological Engineering, College of Life Science, Dongguk University)
  • 이수광 (동국대학교 상명과학대학 환경생태공학과 환경생명공학연구실) ;
  • 김지희 (동국대학교 상명과학대학 환경생태공학과 환경생명공학연구실) ;
  • 강호덕 (동국대학교 상명과학대학 환경생태공학과 환경생명공학연구실)
  • Received : 2008.02.26
  • Accepted : 2008.03.17
  • Published : 2008.03.30
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Abstract

This study was conducted to establish the optimal condition for plant regeneration and acclimatization from somatic embryos of Panax ginseng. Cotyledon segments of Panax ginseng produced primary and secondary somatic embryos when cultured on MS and WPM media supplemented with 7% sucrose. To induce plantlet conversion, cotyledonary somatic embryos were cultured on WPM solid medium with $GA_3$ at various concentrations (1~30 mg/L) for 4 weeks. Plantlets were transferred to 1/2 WPM solid medium with $GA_3$ at various concentrations (0~5 mg/L) and 0.5% activated charcoal for shoot and root elongations. Elongated plantlets further developed into well-developed leaf and root system on 1/3 SH medium with 0.5% activated charcoal under ventilation condition for 5 months. The highest survival rate to soil was 75% when plantlets were regenerated on 1/3 SH medium without sucrose under ventilation condition.

본 연구는 장뇌삼(Panax ginseng)의 효율적인 증식과 성공적인 토양 순화의 최적 조건을 구명하기 위해 수행되었다. 장뇌삼의 자엽을 시료로 사용하여 체세포배를 유도한 후, 유도된 체세포배를 발아시켜 얻은 자엽을 다시 시료로 사용하여 2차 체세포배를 유도함으로써 효율성을 증가시켰다. 2차 체세포배의 형성율은 MS 배지의 경우 약 53%이고, WPM 배지의 경우 약 48%인 것으로 나타나 1차 체세포 배의 형성율인 MS 배지와 WPM 배지의 각각 83%, 73%에 비해 약간 떨어지는 것으로 나타났다. 형성된 체세포배는 $GA_3$가 첨가된 WPM 배지에서 발아시키고, 무기염류의 양을 반감시키고, $GA_3$가 첨가된 WPM 배지에서 신장시킨 후, 무기염류를 줄인 SH 배지에서 대기 순화와 무당배양의 처리에 따른 줄기와 뿌리길이, 생존율 차이를 조사하였다. 기내에서 대기 순화와 무당배양 혼용처리 시 가장 정상적인 유식물체로 성장 시킬 수 있었고, 뿌리길이도 가장 길었으며, 생존율 또한 75%로써 가장 높은 생존율을 보였다.

Keywords

Acknowledgement

Supported by : 동국대학교

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