식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제38권1호
  • /
  • Pages.51-61
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  • 2008
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
권호 표지

잎의 발생과정에 있어서의 극성제어

Regulation of Leaf Polarity during Leaf Development

  • 조규형 (동아대학교 분자생명공학부) ;
  • 전상은 (동아대학교 분자생명공학부) ;
  • ;
  • 김경태 (동아대학교 분자생명공학부)
  • Cho, Kiu-Hyung (Division of Molecular Biotechnology, Dong A University) ;
  • Jun, Sang-Eun (Division of Molecular Biotechnology, Dong A University) ;
  • Tsukaya , Hirokazu (Graduate School of Science, University of Tokyo) ;
  • Kim, Gyung-Tae (Division of Molecular Biotechnology, Dong A University)
  • 투고 : 2007.11.21
  • 심사 : 2007.11.28
  • 발행 : 2008.03.31
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초록

잎은 무한생장기관으로 잎의 극성제어에 많은 유전적인 요소가 필요하다. 이들 극성은 잎의 초기발생과정에서 제어되기 시작하고, 정단분열조직과 잎기관의 원기와의 제어를 담당하는 인자들에 의해서 결정이 된다. 본 연구에서는 가늘고 바늘처럼 생긴 잎을 가진 deformed root and leaf1 (drl1) 돌연변이체를 유전학적 해석하였고, 그 결과 DRL1 유전자는 정단분열조직과 잎의 극성축을 제어하고 있는 것으로 판명되었다. 이 DRL1 유전자는 효모의 KTI12 유전자 산물과 유사한 단백질인 Elongator associate protein을 만들어 내는 것으로 판명되었다. 또한, 이 단백질의 아미노산 서열이 원핵생물에서부터 진핵생물까지 광범위하게 진화적으로 보존되고 있는 것으로 밝혀졌다. 특히, DRL1 단백질과 유사한 식물의 단백질은 계통해석 결과 단일계통을 나타내고 있는 것으로 나타났고, 이는 이 단백질들이 육상식물의 진화과정에서 잘 보존되고 있음을 시사하고 있다.

Leaves are indeterminate organs and possess a lot of genes which is involved in establishing leaf polarities. These polarities are regulated relatively early during leaf development and defined relative to the factors intrinsic to the primordia and interactions with the shoot apical meristem (SAM). Recently, several genes that control the polarity of lateral organs have been identified. Our genetic study of deformed root and leaf1 (drl1) mutant, which produces narrow, filament‐like leaves and defective meristems, revealed that DRL1 is involved in the regulation of SAM activity and leaf polarity. The DRL1 gene was found to encode a novel protein showing homology to Elongator‐associate protein (EAP) of yeast KTI12. The amino acid sequence of DRL1 is universally conserved in prokaryotes and eukaryotes. DRL1 and the plant DRL1 homologs clearly formed a monophyletic clade, suggesting the evolutionary conservation of DRL1 homologs was maintained in the genomes of all land plants.

키워드

과제정보

연구 과제 주관 기관 : Korea Research Foundation, Environmental Biotechnology National Core Research Center

참고문헌

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