Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제34권6호
  • /
  • Pages.635-639
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  • 2008
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

다양한 소독방법이 PPDO-hybrid-PLGA nanofiber scaffold의 형태와 세포부착에 미치는 영향에 관한 연구

EFFECT OF DIFFERENT STERILIZATION METHODS ON THE SURFACE MORPHOLOGY OF PPDO-hybrid-PLGA NANOFIBER SCAFFOLD AND ATTACHMENTS OF PC12 CELL

  • 이주현 (이화여자대학교 의학전문대학 구강악안면외과) ;
  • 민현기 (이화여자대학교 의학전문대학 구강악안면외과) ;
  • 정주영 (이화여자대학교 임상치의학대학원 임플란트치의학과) ;
  • 강나라 (이화여자대학교 의학전문대학 구강악안면외과)
  • Lee, Ju-Hyon (Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery) ;
  • Min, Hyun-Gi (Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery) ;
  • Jung, Ju-Young (Ewha Womans University Graduate School of Clinical Dentistry Department of lmplant Dentistry) ;
  • Kang, Na-Ra (Ewha Womans University School of Medicine Department of Oral and Maxillofacial Surgery)
  • 발행 : 2008.12.31
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초록

Objectives : the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated. Methods : Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; Control:Non-sterilized scaffold, Group I:scaffold sterilized with 70% Alcohol, Group II: scaffold sterilized with Ethylene Oxide at $65^{\circ}C$, and Group III: scaffold sterilized with Ethylene Oxide at $37^{\circ}C$. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control: sterilized with 70% Alcohol, Group I: sterilized with Ethylene Oxide at $65^{\circ}C$, and Group II: sterilized with Ethylene Oxide at $37^{\circ}C$. Results : 1. The surface morphology was slightly changed in Group I, II and Group III, compared with control. 2. The attachment of PC12 cells in Group I, II was not higher than in control Discussion : The attachment of PC12 cell is not influenced by different sterilization methods.

키워드

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