DOI QR코드

DOI QR Code

모유 섭취 신생아 유래 Lactobacillus acidophilus에 의한 발효유 내 중미형성

Production of Flavor Compounds in Fermented Milk by Lactobacillus acidophilus Isolated from Breast-Fed Infants

  • 박정규 (단국대학교 동물자원학과) ;
  • 송원호 (단국대학교 동물자원학과) ;
  • 홍성문 (단국대학교 동물자원학과) ;
  • 김철현 (단국대학교 동물자원학과)
  • Park, Jeong-Gyu (Department of Animal Resource & Science, Dankook University) ;
  • Song, Won-Ho (Department of Animal Resource & Science, Dankook University) ;
  • Hong, Sung-Moon (Department of Animal Resource & Science, Dankook University) ;
  • Kim, Cherl-Hyun (Department of Animal Resource & Science, Dankook University)
  • 발행 : 2008.12.31

초록

이 연구는 모유를 섭취하고 있는 생후 7일 이내의 신생아로부터 분리 및 선발된 9종의 L. acidophilus를 이용하여 제조된 발효유의 풍미성분의 확인을 통해 상업적 발효유제조 시 사용가능성을 탐색하고자 수행하였다. 시험 균주에 의한 유리아미노산의 생성은 전반적으로 arginine 및 histidine과 같은 쓴맛을 내는 유리아미노산의 생성량이 높았으며, 유기산은 lactic acid의 경우 전 처리구에서 다른 유기산에 비해 높게 생성되었고, 전체적인 유기산 생성량은 L. acidophilus NB 209 처리구가 가장 높았다. 또한 acetaldehyde의 함량이 다른 휘발성 향미성분에 비해 높게 생성되었으며 L. acidophilus NB 209에 의한 acetaldehyde 생성량이 가장 높게 나타났다. 시험 균주로 발효유를 제조하여 관능검사를 실시한 결과 맛과 향미는 L. acidophilus NB 209로 제조된 발효유에 대한 기호도가 다른 처리군에 비해 높게 나타났으나 조직감은 다른 시험 균주에 비해 조금 낮게 나타났고, 상업적 발효유 제조에 사용 중인 L. acidophilus 145에 비해 전반적으로 기호도가 낮게 나타났다. 따라서 L. acidophilus NB 209의 제품 적용가능성이 가장 높은 것으로 나타났으나 상업적인 이용을 위해 향후 최적배양조건 선정 실험을 통한 관능성의 개선 관련 연구가 필요한 것으로 생각된다.

Lactobacillus acidophilus is a normal inhabitant of the human intestine and its numerous health benefits have been reported. This organism is referred to as a "starter culture". This study was conducted to verify that the production of flavor compounds in fermented milk was obtained using a good probiotic strain of L. acidophilus from breast-fed infant feces. The bitter-tasting amino acids, such as arginine and histidine were produced in larger amounts than other free amino acids when L. acidophilus strains were inoculated in skim milk. The lactic acid was the major acid produced from glucose. L. acidophilus NB 209 was the best producer of lactic acid. This L. acidophilus NB 209 produced higher amounts of acetaldehyde than other L. acidophilus strains. L. acidophilus NB 209 gave higher flavor and taste score of the yogurt produced than other L. acidophilus strains in sensory evaluation. These results indicate that L. acidophilus NB 209 has the potential to be developed as a starter culture for fermented milk products.

키워드

참고문헌

  1. Bassette, R. and Ward, G. (1975) Measuring parts per billion of volatile materials in milk. J. Dairy Sci. 58, 428-435 https://doi.org/10.3168/jds.S0022-0302(75)84583-8
  2. Beshkova, D., Simona, E., Frengova, G., and Simov, Z. (1998) Production of flavor compounds by yogurt starter culture. J. Industrial Microbiol. Biotechnol. 20, 180-190 https://doi.org/10.1038/sj.jim.2900504
  3. Bevilacqua, A. E. and Califano, A. N. (1989) Determination of organic acids in dairy products by high performance liquid chromatography. J. Food Sci. 54(4), 1076-1079 https://doi.org/10.1111/j.1365-2621.1989.tb07948.x
  4. Bottazzi, V. and Vescovo, M. (1969) Carbonyl compounds produced by yogurt bacteria. Neth. Milk Dairy J. 23, 71-77
  5. Conway, P. L., Gorbach, S. L., and Goldin, B. R. (1987) Survival of lactic acid bacteria in the human stomach and adhesion to intestinal cells. J. Dairy Sci. 70, 1-12 https://doi.org/10.3168/jds.S0022-0302(87)79974-3
  6. Fox, P. F. (1983) Developments in dairy chemistry I. Applied Science Publication, NY, pp. 218-220
  7. Gilliland, S. E. (1989) Acidophilus milk producers: a review of potential benefits to consumers. J. Dairy Sci. 72, 2483-2494 https://doi.org/10.3168/jds.S0022-0302(89)79389-9
  8. Gonzalez, S., Ambrosini, V. M., Nadra, M. M., Ruiz Holgado, A., and Oliver, G. (1994) Acetaldehyde production by strains used as probiotics in fermented milk. J. Food Prot. 57, 436-440 https://doi.org/10.4315/0362-028X-57.5.436
  9. Hoier, E. (1992) Use of probiotic starter cultures in dairy products. Food Tech. Australia 44, 418-420
  10. Holzapfel, W. H. and Schillinger, U. (2002) Introduction to pre-and probiotics. Food Res. Int. 35, 104-116
  11. Hood, S. K. and Zottola, E. A. (1988) Effect of low pH on the ability of Lactobacillus acidophilus to survive and adhere to human intestinal cells. J. Food Sci. 53, 1514-1516 https://doi.org/10.1111/j.1365-2621.1988.tb09312.x
  12. Imhof, R., Glatti, H., and Basset, J. O. (1995) Volatile organic compounds produced by thermophilic and mesophilic single strain dairy starter cultures. Lebensm. Wiss. Techol. 28, 78-85 https://doi.org/10.1016/S0023-6438(95)80016-6
  13. Ishibashi, N. and Shimamura, S. (1993) Bifidobacteria: Research and development in Japan. Food Technol. 47, 129-134
  14. Kang, Y. J., Frank, J. F., and Lilard, D. A. (1988) Gas chromatographic detection of yogurt flavor compounds and changes during refrigerated storage. Cultured Dairy Prod. J. 23, 6-12
  15. Kim, C. H., Shin, Y. K., Baick, S. C., and Kim, S. K. (1999) Changes of oligosaccharide and free amino acid in soy yogurt fermented with different mixed culture. Kor. J. Food Sci. Technol. 31(3), 739-745
  16. Laye, I., Karleskind, D., and Morr, V. V. (1993) Chemical, Microbiological and Sensory properties of plain nonfat yogurt. J. Food Sci. 58, 991-997 https://doi.org/10.1111/j.1365-2621.1993.tb06096.x
  17. Lee, K. W., Shin, Y. K., and Baick, S. C. (1998) D(-) and L(+)-Lactic acid determination of Lactobaillus acidophilus during fermentation and storage period. Kor. J. Food Sci. Technol. 30(1), 168-174
  18. Lew, I. D. and Yu, J. H. (1987) Interaction between Lactobacillus acidophilus and Kluyveromyces fragilis on the metabolism of galacto-oligosaccharides in soy milk. Kor. J. Appl. Microbiol. Bioeng. 15, 253-260
  19. Libeck, A., Gustafsson, J., and Nord, C. E. (1987) Impact of Lactobacillus acidophilus supplements on the human intestinal microflora. Scand. J. Infect. Dis. 19, 531-537 https://doi.org/10.3109/00365548709032419
  20. Liu, H. J. (1994) Determination of amino acids by precolumn derivatization with 6-aminoguinoly-N-hydroxysuccinimidyl carbamate and HPLC with ultraviolet detection. J. Chromatography A 670, 59-66 https://doi.org/10.1016/0021-9673(94)80280-7
  21. Lourens-Hattingh, A. and Viljoen, B. C. (2001) Yogurt as probiotic carrier food. Int. Dairy J. 11, 1-17 https://doi.org/10.1016/S0958-6946(01)00036-X
  22. Marshall, V. M. and Cole, W. M. (1983) Threonine aldolase and alcohol dehydrogenase activities in Lactobacillus bulgaricus and Lactobacillus acidophilus and their contribution to flavour production in fermented milks. J. Dairy Res. 46, 42-57
  23. Oberman, H. and Libudzisz, Z. (1998) Fermented milks. In: Wood, B. J. B. ed. Microbiology of fermented foods. Blackie Academic and Professional. London. pp. 309-350
  24. Olieman, C. and de Vries, E. S. (1988) Determination of Dand L-lactic acid in fermented dairy products with HPLC. Neth. Milk Dairy J. 42, 111-116
  25. Ostlie, H. M., Helland, M. H., and Narvhus, J. A. (2003) Growth and metabolism of selected strains of probiotic bacteria in milk. Int. J. Food Microbiol. 87, 17-27 https://doi.org/10.1016/S0168-1605(03)00044-8
  26. Park, C. K., Lew, I. D., and Yu, J. H. (1986) Effect of peptide on the mixed fermentation of Lactobacillus helveticus YH-1 and Streptococcus lactic ML3 in skim milk. Kor. J. Appl. Microbiol. Bioeng. 14, 487-493
  27. Rasic, J. L. and Kurmann, J. A. (1978) Yogurt: Scientific grounds, technology, manufacture and preparations. Technical Dairy Publishing House, Copenhagen, p. 90-97
  28. Salminen, S., Laine, M., Wright, A., Vuopiovarkila, J., Korhonen, T., and Mattila-Sandholm, T. (1996) Development of selection criteria for probiotic strains to assess their potential in functional foods: a nordic and european approach. Bioscience Microflora 15, 61-67 https://doi.org/10.12938/bifidus1996.15.61
  29. Samona, A., Robinson, R. K., and Marakis, S. (1996) Acid production by bifidobacteria and yoghurt bacteria during fermentation and storage of milk. Food Microbiol. 13, 275-280 https://doi.org/10.1006/fmic.1996.0033
  30. SAS (1996) SAS/STAT Software for PC. Release 6.11, SAS Institute Inc., Cary, NC, USA
  31. Shankar, P. A. (1977). Dairy starter culture. Ph. D. thesis, University of Reading, Reading, England
  32. Takiguchi, R., Mochizuki, E., Suzuki, Y., Nakajima, I., and Benno, Y. (1997) SBT 2062 and Bifidobacterium longum SBT 2928 on harmful intestinal bacteria. J. Int. Microbiol. 11, 11-17
  33. Tamine, A. Y. and Robinson, P. K. (1999) Yoghurt: Science and Technology. Woodhead Publishing, Cambridge, England, pp. 433-485