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Physical Property and Stability of Liposome Prepared from Egg Yolk Phospholipids at Various Storage Conditions

난황 유래 인지철로 합성한 리포좀의 물리적 특성과 안정성

  • Park, Sun-Hyun (Division of Food Science/Technology and Food Service Industry, Yeungnam University) ;
  • Kim, Myung-Hee (Division of Food Science/Technology and Food Service Industry, Yeungnam University)
  • 박선현 (영남대학교 식품.외식학부 식품가공학) ;
  • 김명희 (영남대학교 식품.외식학부 식품가공학)
  • Published : 2008.12.31

Abstract

Liposomes were prepared from egg yolk phospholipids to study their physical properties and stability at various storage conditions. Under storage conditions at different pH levels, the particle sizes of liposomes increased at the range of pH 1-2, and the absolute values of $\xi$-potentials were reduced at the range of pH 1-4. The leakage of sulforhodamine B (SRB), a fluorescent dye which is encapsulated in the liposome, increased greatly at pH 2-4. At different storage temperatures, the particle size of liposomes increased from the 10 days of storage at $4^{\circ}C$ and the 40 days at 20 and $35^{\circ}C$. The $\xi$-potentials decreased slightly later during storage under 4, 20 and $35^{\circ}C$. At the storage temperature of $50^{\circ}C$, the leakage of SRB was the greatest. Therefore, we concluded that the pH conditions lower than pH 6 and high temperature of $50^{\circ}C$ are not conducive to storing liposomes. The results obtained here may prove helpful in developing liposome-based encapsulation and diagnostic reagents.

난황 유래의 인지질을 주원료로 리포좀을 제조하여 서로 다른 저장조건에서의 리포좀의 물리적인 안정성을 연구하였다. 리포좀 용액의 pH를 달리하여 저장한 후 리포좀 입자크기와 리포좀 용액의 제타전위와 형광도를 측정한 결과, 리포좀은 pH 1-2에서 입자크기의 증가를 나타내었고 pH 4 이하에서 제타전위의 절대값이 감소하였으며 리포좀의 내부에 포집한 수용성물질의 손실량 지표인 형광도의 측정치 또한 pH 2-4에서 급격하게 증가한 것으로 보아 산성의 저장조건에서는 리포좀이 불안정함을 추론할 수 있다. 저장 온도별 리포좀의 안정성 평가에서는 $4^{\circ}C$에서 저장하였을 때 10일째부터 입자의 크기 변화가 있었고 20, $35^{\circ}C$에서 저장할 경우, 저장기간 40일째 이후부터 입자의 크기변화가 있었다. 제타전위는 3가지 온도조건에서 동일하게 저장 말기에 측정값의 절대값이 미세하게 감소하는 경향을 보였다. 또한, $50^{\circ}C$에서 저장된 리포좀의 형광도 측정실험에서 포집된 SRB의 누출량이 급격히 증가하는 양상을 나타내었다. 따라서 pH 6 이하의 산성의 조건에서 리포좀은 불안정하며 $50^{\circ}C$와 같이 높은 온도에서 인지질로 구성된 리포좀의 안정성을 유지하기 어렵다는 것을 알 수 있었다. 본 연구의 결과는 난황 유래 인지질로 구성된 리포좀을 이용한 진단용 시약 개발 및 포집 특성 연구에 유용한 기초 자료로 활용될 수 있을 것이다.

Keywords

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