Journal of Microbiology and Biotechnology

  • 제18권12호
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  • Pages.1895-1902
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  • 2008
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Proteome Analysis for 3T3-L1 Adipocyte Differentiation

  • 발행 : 2008.12.31
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초록

Adipose tissue is an important endocrine organ involved in the control of whole body energy homeostasis and insulin sensitivity. Considering the increased incidence of obesity and obesity-related disorders, including diabetes, it is important to understand thoroughly the process of adipocyte differentiation and its control. Therefore, we performed a differential proteome mapping strategy using two-dimensional gel electrophoresis combined with peptide mass fingerprinting to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3-L1 pre-adipocytes in response to an adipogenic cocktail. In the current study, we identified 46 differentially expressed proteins, 6 of which have not been addressed previously in 3T3-L1 cell differentiation. Notably, we found that phosphoribosyl pyrophosphate synthetase (PRPS), a regulator of cell proliferation, was preferentially expressed in pre-adipocytes than in fully differentiated adipocytes. In conclusion, our results provide valuable information for further understanding of the adipogenic process.

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참고문헌

  1. Becker, M. A., P. Dicker, and E. Rozengurt. 1983. Mitogenic enhancement of purine base phosphoribosylation in Swiss mouse 3T3 cells. Am. J. Physiol. 244: 288-296 https://doi.org/10.1152/ajpcell.1983.244.3.C288
  2. Biddinger, S. B. and C. R. Kahn. 2006. From mice to men: Insights into the insulin resistance syndromes. Annu. Rev. Physiol. 68: 123-158 https://doi.org/10.1146/annurev.physiol.68.040104.124723
  3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  4. Burton, G. R., Y. Guan, R. Nagarajan, and R. E. McGehee Jr. 2002. Microarray analysis of gene expression during early adipocyte differentiation. Gene 293: 21-31 https://doi.org/10.1016/S0378-1119(02)00726-6
  5. Choi, K. L., Y. Wang, C. A. Tse, K. S. Lam, G. J. Cooper, and A. Xu. 2004. Proteomic analysis of adipocyte differentiation: Evidence that alpha2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytes. Proteomics 4: 1840-1848 https://doi.org/10.1002/pmic.200300697
  6. DeRossi, C., L. Bode, E. A. Eklund, F. Zhang, J. A. Davis, V. Westphal, L. Wang, A. D. Borowsky, and H. H. Freeze. 2006. Ablation of mouse phosphomannose isomerase (Mpi) causes mannose 6-phosphate accumulation, toxicity, and embryonic lethality. J. Biol. Chem. 281: 5916-5927 https://doi.org/10.1074/jbc.M511982200
  7. Futcher, B., G. I. Latter, P. Monardo, C. S. McLaughlin, and J. I. Garrels. 1999. A sampling of the yeast proteome. Mol. Cell. Biol. 19: 7357-7368 https://doi.org/10.1128/MCB.19.11.7357
  8. Geigl, J. B., S. Langer, S. Barwisch, K. Pfleghaar, G. Lederer, and M. R. Speicher. 2004. Analysis of gene expression patterns and chromosomal changes associated with aging. Cancer Res. 64: 8550-8557 https://doi.org/10.1158/0008-5472.CAN-04-2151
  9. Gregoire, F. M. 2001. Adipocyte differentiation: From fibroblast to endocrine cell. Exp. Biol. Med. 226: 997-1002 https://doi.org/10.1177/153537020122601106
  10. Gregoire, F. M., C. M. Smas, and H. S. Sul. 1998. Understanding adipocyte differentiation. Physiol. Rev. 78: 783-809 https://doi.org/10.1152/physrev.1998.78.3.783
  11. Guo, X. and K. Liao. 2000. Analysis of gene expression profile during 3T3-L1 preadipocyte differentiation. Gene 251: 45-53 https://doi.org/10.1016/S0378-1119(00)00192-X
  12. Gygi, S. P., Y. Rochon, B. R. Franza, and R. Aebersold. 1999. Correlation between protein and mRNA abundance in yeast. Mol. Cell. Biol. 19: 1720-1730 https://doi.org/10.1128/MCB.19.3.1720
  13. Hovi, T., A. C. Allison, K. Raivio, and A. Vaheri. 1977. Purine metabolism and control of cell proliferation. Ciba Found Symp. 48: 225-248
  14. Juan, H. F., J. Y. Lin, W. H. Chang, C. Y. Wu, T. L. Pan, M. J. Tseng, K. H. Khoo, and S. T. Chen. 2002. Biomic study of human myeloid leukemia cells differentiation to macrophages using DNA array, proteomic, and bioinformatic analytical methods. Electrophoresis 23: 2490-2504 https://doi.org/10.1002/1522-2683(200208)23:15<2490::AID-ELPS2490>3.0.CO;2-3
  15. Kaida, A., Y. Ariumi, K. Baba, M. Matsubae, T. Takao, and K. Shimotohno. 2005. Identification of a novel p300-specificassociating protein, PRS1 (phosphoribosylpyrophosphate synthetase subunit 1). Biochem. J. 39: 239-247
  16. Kandror, K. V. and P. F. Pilch. 1996. The insulin-like growth factor II/mannose 6-phosphate receptor utilizes the same membrane compartments as GLUT4 for insulin-dependent trafficking to and from the rat adipocyte cell surface. J. Biol. Chem. 271: 21703-21708 https://doi.org/10.1074/jbc.271.36.21703
  17. Kopelman, P. G. 2000. Obesity as a medical problem. Nature 404: 635-643 https://doi.org/10.1038/35007508
  18. Moon, H. S., C. S. Chung, H. G. Lee, T. G. Kim, Y. J. Choi, and C. S. Cho. 2007. Inhibitory effect of (-)-epigallocatechin-3-gallate on lipid accumulation of 3T3-L1 cells. Obesity 15: 2571-2582 https://doi.org/10.1038/oby.2007.309
  19. Nadler, S. T., J. P. Stoehr, K. L. Schueler, G. Tanimoto, B. S. Yandell, and A. D. Attie. 2000. The expression of adipogenic genes is decreased in obesity and diabetes mellitus. Proc. Natl. Acad. Sci. USA 97: 11371-11376 https://doi.org/10.1073/pnas.97.21.11371
  20. Ntambi, J. M. and Y. C. Kim. 2000. Adipocyte differentiation and gene expression. J. Nutr. 130: 3122-3126 https://doi.org/10.1093/jn/130.12.3122S
  21. Rahman, M. A., S. G. Kumar, S. W. Kim, H. J. Hwang, Y. M. Baek, S. H. Lee, H. S. Hwang, Y. H. Shon, K. S. Nam, and J. W. Yun. 2008. Proteomic analysis for inhibitory effect of chitosan oligosaccharides on 3T3-L1 adipocyte differentiation. Proteomics 8: 569-581 https://doi.org/10.1002/pmic.200700888
  22. Renes, J., F. Bouwman, J. P. Noben, C. Evelo, J. E. Robben, and E. Mariman. 2005. Protein profiling of 3T3-L1 adipocyte differentiation and (tumor necrosis factor $\alpha$-mediated) starvation. Cell. Mol. Life Sci. 62: 492-503 https://doi.org/10.1007/s00018-004-4498-9
  23. Rheem, D. M. 2002. Microarray-based expression profiling of normal and malignant immune cells. Endocr. Rev. 23: 393-400 https://doi.org/10.1210/er.23.3.393
  24. Richards, J., F. Le Naour, S. Hanash, and L. Beretta. 2002. Integrated genomic and proteomic analysis of signaling pathways in dendritic cell differentiation and maturation. Ann. NY Acad. Sci. 975: 91-100 https://doi.org/10.1111/j.1749-6632.2002.tb05944.x
  25. Rosen, E. D. and B. M. Spiegelman. 2000. Molecular regulation of adipogenesis. Annu. Rev. Cell Dev. Biol. 16: 145-171 https://doi.org/10.1146/annurev.cellbio.16.1.145
  26. Schneider, R., E. Schneider-Scherzer, M. Thurnher, B. Auer, and M. Schweiger. 1988. The primary structure of human ribonuclease/ angiogenin inhibitor (RAI) discloses a novel highly diversified protein superfamily with a common repetitive module. EMBO J. 7: 4151-4156
  27. Shapiro, R. and B. L. Vallee. 1987. Human placental ribonuclease inhibitor abolishes both angiogenic and ribonucleolytic activities of angiogenin. Proc. Natl. Acad. Sci. USA 84: 2238-2241 https://doi.org/10.1073/pnas.84.8.2238
  28. Shevchenko, A., M. Wilm, O. Vorm, and M. Mann. 1996. Mass spectrometric sequencing of proteins from silver stained gels. Anal. Chem. 68: 850-858 https://doi.org/10.1021/ac950914h
  29. Sidhu, R. S. 1979. Two-dimensional electrophoretic analyses of proteins synthesized during differentiation of 3T3-L1 preadipocytes. J. Biol. Chem. 254: 11111-11118
  30. Sorisky, A. 1999. From preadipocyte to adipocyte: Differentiationdirected signals of insulin from the cell surface to the nucleus. Crit. Rev. Clin. Lab Sci. 36: 1-3 https://doi.org/10.1080/10408369991239169
  31. Soukas, A., N. D. Socci, B. D. Saatkamp, S. Novelli, and J. M. Friedman. 2001. Distinct transcriptional profiles of adipogenesis in vivo and in vitro. J. Biol. Chem. 276: 34167-34174 https://doi.org/10.1074/jbc.M104421200
  32. Takahashi, M. 2001. Adipocytes as endocrine organ-adipocytokines and lipid metabolism. J. Clin. Med. 59: 504-508
  33. Tatibana, M., K. Kita, M. Taira, S. Ishijima, T. Sonoda, T. Ishizuka, T. Iizasa, and I. Ahmad. 1995. Mammalian phosphoribosylpyrophosphate synthetase. Adv. Enzyme Regul. 35: 229-249 https://doi.org/10.1016/0065-2571(94)00017-W
  34. Welsh, G. I., M. R. Griffiths, K. J. Webster, M. J. Page, and J. M. Tavare. 2004. Proteome analysis of adipogenesis. Proteomics 4: 1042-1051 https://doi.org/10.1002/pmic.200300675
  35. Wilson-Fritch, L., A. Burkart, G. Bell, K. Mendelson, J. Leszyk, S. Nicoloro, M. Czech, and S. Corvera. 2003. Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone. Mol. Cell. Biol. 23: 1085-1094 https://doi.org/10.1128/MCB.23.3.1085-1094.2003
  36. Wu, Z., P. Puigserver, and B. M. Spiegelman. 1999. Transcriptional activation of adipogenesis. Curr. Opin. Cell Biol. 11: 689-694 https://doi.org/10.1016/S0955-0674(99)00037-X
  37. Yang, Y., W. Shang, L. Zhou, B. Jiang, H. Jin, and M. Chen. 2007. Emodin with PPARgamma ligand-binding activity promotes adipocyte differentiation and increases glucose uptake in 3T3-Ll cells. Biochem. Biophys. Res. Commun. 353: 225-230 https://doi.org/10.1016/j.bbrc.2006.11.134

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