Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Padina arborescens on $H_2O_2$-induced Oxidative Stress in Human Endothelial Cell line, ECV304 cells

부챗말 추출액이 $H_2O_2$에 의한 혈관내피세포주인 ECV304세포의 산화적 스트레스에 미치는 영향

  • Park, Jin-Mo (Division of Natural Science, College of Natural Sciences) ;
  • Ju, Sung-Min (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Jeon, Byung-Jae (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Yang, Hyun-Mo (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Choi, Han-Kil (Division of Natural Science, College of Natural Sciences) ;
  • Jeon, Byung-Hoon (Department of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Kim, Won-Sin (Institute of Biotechnology, Wonkwang University)
  • 박진모 (원광대학교 자연과학대학 생명과학부) ;
  • 주성민 (원광대학교 한의과대학 병리학교실) ;
  • 전병제 (원광대학교 한의과대학 병리학교실) ;
  • 양현모 (원광대학교 한의과대학 병리학교실) ;
  • 최한길 (원광대학교 자연과학대학 생명과학부) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 김원신 (원광대학교 생명공학연구소)
  • Published : 2008.12.25
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Abstract

The marine algae, Padina arborescens, have been used traditionally for treatment of various brain diseases. However, the molecular studies on the effect of Padina arborescens have not been carried out. In the present study, the protective effect of the water extract of Padina arborescens (PAWE) was researched in $H_2O_2$-treated human vascular endothelial cells, ECV304. ECV304 cells were pre-incubated with PAWE (0, 400, 800, 1,200 and $1,600{\mu}g/m{\ell}$) for 12 h and treated with 500 uM $H_2O_2$ for 12 h, and then the protective effects of PAWE were determined. PAWE recovered the $H_2O_2$-induced cell damage and decreased ROS production in ECV304 cells. Moreover, PAWE increased ERK expression and inhibited p38 and JNK expression. Furthermore, PAWE dosedependently increased the expression of heme oxygenase-1 (HO-1) and the HO-1 expression was reduced by ERK inhibitor treatment in $H_2O_2$-treated EVC304 cells. These results suggested that protective effect of PAWE on $H_2O_2$-induced oxidative stress in ECV304 cells might be associated with the production of HO-1 through the ERK signal pathway.

Keywords

References

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