Effect of Sopung-tang on Glutamate-Induced Apoptosis in C6 Glial Cells

소풍탕(疎風湯)이 Glutamate에 의한 C6 Glial Cell의 Apoptosis에 미치는 영향

  • Jeong, Seung-Won (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi, Chul-Won (Department of Internal Medicine, School of Oriental Medicine, Wonkwang University) ;
  • Kim, Bong-Sang (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Moon, Byung-Soon (Department of Third Medicine, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 정승원 (원광대학교 한의학전문대학원 제3의학과) ;
  • 최철원 (원광대학교 한의과대학 내과학교실) ;
  • 김봉상 (원광대학교 한의학전문대학원 제3의학과) ;
  • 문병순 (원광대학교 한의학전문대학원 제3의학과)
  • Published : 2008.12.25

Abstract

The water extract of Sopung-tang(SPT) has been traditionally used for treatment of psycologic disease and brain damage in oriental medicine. However, little is known about the mechanism by which the water extract of SPT rescues cells from these disease. Therefore, this study was designed to investigate the effect of SPT on the glutamate-induced toxicity of rat C6 glial cells. SPT have protective effects in glutamate-induced toxicity, which was revealed as apoptosis characterized by chromatic condensation and fragmentation and the loss of mitochondrial membrane potential in C6 glial cells. Also, SPT have inhibited the active form of caspase-3 and PARP and significantly protected the apoptotic phenomena by glutamate toxicity in C6 glial cells. However, SPT significantly recovered the depletion of GSH and inhibited the generation of ROS by glutamate in C6 glial cells. In addition, both SPT and antioxidants such as GSH and NAC protected the glutamate-induced cytotoxicity in C6 glial cells, indicating that SPT possibly have antioxidative effect. Specially, SPT were showed transcriptional factor significantly increased the activation of NF-${\kappa}B$ using the analysis of NF-${\kappa}B$ luciferase reporter system in C6 glial cells. These NF-${\kappa}B$ activation protected cells from glutamate-induced toxicity to generate the heme oxygenase-1(HO-1). Taken together, we suggest that SPT have protective effects in glutamate-induced toxicity via a antioxidative mechanism.

Keywords

References

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