Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Reactivity of German Cockroach Allergen, Bla g 2, Peptide Fragments to IgE Antibodies in Patients' Sera

  • Lee, Hae-Seok (Department of Environmental Medical Biology and the Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
  • Jeong, Kyoung-Yong (Department of Environmental Medical Biology and the Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
  • Shin, Kwang-Hyun (Department of Environmental Medical Biology and the Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
  • Yi, Myung-Hee (Department of Environmental Medical Biology and the Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine) ;
  • Gantulaga, Darambazar (Department of Medical Biology, Health Science University) ;
  • Hong, Chein-Soo (Department of Internal Medicine and the Institute of Allergy, Yonsei University College of Medicine) ;
  • Yong, Tai-Soon (Department of Environmental Medical Biology and the Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine)
  • Published : 2008.12.31
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Abstract

Bla g 2 is a cockroach allergen of great importance, This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique, Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2), Only 5,3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients' sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies.

Keywords

References

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