Journal of Periodontal and Implant Science

  • 제38권4호
  • /
  • Pages.621-628
  • /
  • 2008
  • /
  • 2093-2278(pISSN)
  • /
  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
권호 표지

성견의 3급 이개부 병변에서 성형성 합성 펩타이드 젤의 치주재생 능력에 관한 실험적 연구

Experimental study on the periodontal regenerative capacity of moldable synthetic peptide domain gel in degree III furcation defect of beagles

  • 김정범 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 박윤정 (서울대학교 치의학전문대학원 두개악안면재건학교실) ;
  • 이상철 ((주) 나이벡) ;
  • 김태일 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 설양조 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 이용무 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 구영 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 류인철 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 한수부 (서울대학교 치의학전문대학원 치주과학교실) ;
  • 정종평 (서울대학교 치의학전문대학원 치주과학교실)
  • Kim, Jeong-Beom (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Park, Yoon-Jeong (Department of Head and Neck Reconstruction, School of Dentistry, Seoul National University) ;
  • Lee, Sang-Cheol (NIBEC Corp.) ;
  • Kim, Tae-Il (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Seol, Yang-Jo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Gu, Young (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, School of Dentistry, Seoul National University)
  • 발행 : 2008.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Purpose: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. Material and Methods: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. Result: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. Conclusion: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible.

키워드

참고문헌

  1. Page RC, Schroeder HE. Pathogenesis of inflammatory periodontal disease. A summary of current work. Lab Invest 1976;33:235-249
  2. Flemmig TF. Periodontitis Ann Periodontol 1999;4:32-37 https://doi.org/10.1902/annals.1999.4.1.32
  3. Caton J, Zander H. Osseous repair of an infrabony pocket without new attachment of connective tissue. J Clin Periodontol 1976;3:54-58 https://doi.org/10.1111/j.1600-051X.1976.tb01850.x
  4. Garrett S. Periodontal regeneration around natural teeth. Ann Periodontol 1996;1:621-666 https://doi.org/10.1902/annals.1996.1.1.621
  5. Reynolds MA, Aichelmann-Reidy EA, Branch-Mays GL, Gunsolley JC. The efficacy of bone replacement grafts in the treatment of periodontal osseous defects. A systematic review. Ann Periodontol 2003;8:227-265 https://doi.org/10.1902/annals.2003.8.1.227
  6. Melcher AH. On the repair potential of periodontal tissues. J Periodontol 1976;47:256-260 https://doi.org/10.1902/jop.1976.47.5.256
  7. Gottlow J, Nyman S, Lindhe J, Karring T, Wennstrom J. New attachment formation in the human periodontium by guided tissue regeneration. Case reports. J Clin Periodontol 1986;13:604-616 https://doi.org/10.1111/j.1600-051X.1986.tb00854.x
  8. Caffesse RG, Smith BA, Duff B et al. Class II furcations treated by guided tissue regeneration in humans: Case reports. J Periodontol 1990;61:510-514 https://doi.org/10.1902/jop.1990.61.8.510
  9. Cortellini P, Pini Prato G, Baldi C, Clauser C. Guided tissue regeneration with different materials. Int J Periodontics Restorative Dent 1990;10:137-151
  10. Selvig KA, Kersten BG, Chamberlain AD, Wikesjo UM, Nilveus RE. Regenerative surgery of intrabony periodontal defects using ePTFE barrier membranes. Scanning electron microscopic evaluation of retrieved membranes versus clinical healing. J Periodontol 1992;63:974-978 https://doi.org/10.1902/jop.1992.63.12.974
  11. Murphy KG, Cunsolley JC. Guided tissue regeneration for the treatment of periodontal intrabony and furcation defects. A systematic review. Ann Periodontol 2003;8:266-302 https://doi.org/10.1902/annals.2003.8.1.266
  12. Hammarstrom L, Alatli I, Fong CD. Origins of cementum. Oral Dis 1996;2:63-69 https://doi.org/10.1111/j.1601-0825.1996.tb00205.x
  13. Hammarstrom L. Enamel matrix, cementum development and regeneration. J Clin Periodontol 1997;24:658-668 https://doi.org/10.1111/j.1600-051X.1997.tb00247.x
  14. Giannobile WV, Somerman MJ Growth and amelogenin-like factors in periodontal wound healing. A systematic review. Ann Periodontol 2003;8:193-204 https://doi.org/10.1902/annals.2003.8.1.193
  15. Esposito M, Grusovin MG, Coulthard P, Worthington HV. Enamel matrix derivative (Emdogainfor periodontal tissue regeneration in intrabony defects (Review) Cochrane Database Syst Rev 2005;19:CD003875
  16. Butler WT, Ritchie H. The nature and functional significance of dentin extracellular matrix proteins. Int J Dev Biol 1995;39:169-179
  17. Gehron RP, Fedarko NS, Hefferan TE et al. Structure and molecular regulation of bone matrix proteins. J Bone Miner Res 1993;8:S483-S487 https://doi.org/10.1002/jbmr.5650081310
  18. Butler WT. Dentin matrix proteins and dentinogenesis. Connect Tissue Res 1995;33:59-65 https://doi.org/10.3109/03008209509016983
  19. Veis A. Mineral matrix interactions in bone and dentin. J Bone Miner Res 1993;8:S493-S497 https://doi.org/10.1002/jbmr.5650081312
  20. McKee MD , Nanci A. Osteopontin at mineralized tissue interfaces in bone, teeth, and osseointegrated implants: Ultrastructural distribution and implications for mineralized tissue formation, turnover, and repair. Microsc Res Tech 1996; 33:141-164 https://doi.org/10.1002/(SICI)1097-0029(19960201)33:2<141::AID-JEMT5>3.0.CO;2-W
  21. Bosshardt DD, Zalzal S, McKee MD, Nanci A. Developmental appearance and distribution of bone sialoprotein and osteopontin in human and rat cementum. Anat Rec 1998;250:13-33 https://doi.org/10.1002/(SICI)1097-0185(199801)250:1<13::AID-AR3>3.0.CO;2-F
  22. Nanci A. Content and distribution of noncollagenous matrix proteins in bone and cementum: relationship to speed of formation and collagen packing density. J Struct Biol 1999;126:256-269 https://doi.org/10.1006/jsbi.1999.4137
  23. Kawaguchi H, Ogawa T, Kurihara H, Nanci A. Immunodetection of noncollagenous matrix proteins during periodontal tissue regeneration. J Periodont Res 2001;36: 205-213 https://doi.org/10.1034/j.1600-0765.2001.036004205.x
  24. Lee JY, Choo JE, Park HJ et al. Injectable gel with synthetic collagen binding peptide for enhanced osteogenesis in vitro and in vivo. Biochem Biophys Res Commun 2007;357:68-74 https://doi.org/10.1016/j.bbrc.2007.03.106
  25. Jun-Beom Park, Jue-Yeon Lee, Ho-Nam Park et al. Osteopromotion with synthetic oligopeptidecoated bovine bone mineral in vivo. J Periodontol 2007;78:157-163 https://doi.org/10.1902/jop.2007.060200
  26. Yang-Jo Seol, Yoon-Jeong Park, Sang-Cheol Lee et al. Enhanced osteogenic promotion around dental implants with synthetic binding motif mimicking bone morphogenetic protein (BMP) - 2. J Biomed Mater Res 2006;77A: 599-607 https://doi.org/10.1002/jbm.a.30639
  27. Hersel U, Dahmen C, Kessler H. RGD modified polymers: biomaterials for stimulated cell adhesion and beyond. Biomaterials 2003;24:4385-4415 https://doi.org/10.1016/S0142-9612(03)00343-0
  28. Hamp SE, Nyman S, Lindhe J. Periodontal treatment of multirooted teeth. Results after 5 years. J Clin Periodontol 1975;2:126-135 https://doi.org/10.1111/j.1600-051X.1975.tb01734.x
  29. Lindhe J, Pontoriero R, Berglundh T, Araujo M. The effect of flap management and bioresorbable occlusive devices in GTR treatment of degree III furcation defects. An experimental study in dogs. J Clin Periodontol 1995;22:276-283 https://doi.org/10.1111/j.1600-051X.1995.tb00148.x
  30. Donath K, Breuner G. A method for the study of undecalcified bones and teeth with attached soft tissues. The Sage-Schliff (sawing and grinding) technique. J Oral Pathol 1982;11:318-326 https://doi.org/10.1111/j.1600-0714.1982.tb00172.x
  31. Trombelli L, Farina R. Clinical outcomes with bioactive agents alone or in combination with grafting or guided tissue regeneration. J Clin Periodontol 2008;35(s8):117-135 https://doi.org/10.1111/j.1600-051X.2008.01265.x
  32. Liaw L, Skinner MP, Raines EW et al. The adhesive and migratory effects of osteopontin are mediated via distinct cell surface integrins: role of $\alpha$v$\beta$3 in smooth muscle migration to osteopontin. J Clin Invest 1995;95:713-724 https://doi.org/10.1172/JCI117718
  33. Oldberg A, Franzen A, Heinegard D. Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an Arg-Gly-Asp cell binding sequence. Proc Natl Acad Sci USA 1986;83:8819-8823 https://doi.org/10.1073/pnas.83.23.8819
  34. Tye CE, Hunter GK, Goldberg HA. Identification of the type I collagen-binding domain of bone sialoprotein and characterization of the mechanism of interaction. J Biol Chem 2005;280:13487-13492 https://doi.org/10.1074/jbc.M408923200
  35. Ito S, Saito T, Amano K. In vitro apatite induction by osteopontin: interfacial energy for hydroxyapatite nucleation on osteopontin. J Biomed Mater Res A 2004;69:11-16
  36. Araujo M, Berglundh T, Lindhe J. The periodontal tissues in healed degree III furcation defects. An experimental study in dogs. J Clin Periodontol 1996;23:532-541 https://doi.org/10.1111/j.1600-051X.1996.tb01821.x
  37. Araujo MG, Lindhe J. GTR treatment of degree III furcation defects following application of enamel matrix proteins. An experimental study in dogs. J Clin Periodontol 1998;25:524-530 https://doi.org/10.1111/j.1600-051X.1998.tb02482.x