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Differentiation Among Commercial Strains of Pleurotus spp. Based on DNA Fingerprinting Using Universal Rice Primer (URP)

Universal Rice Primer(URP)에 의한 DNA 핵산지문법을 이용한 느타리의 유통 품종간 구분

  • Seo, Kyoung-In (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jang, Kab-Yeul (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Park, Soon-Young (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Kwang-Ho (Department of Crop Science, KonKuk University) ;
  • Kong, Won-Sik (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 서경인 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 박순영 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 김광호 (건국대학교 식량자원학과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과)
  • Published : 2008.12.31

Abstract

To distinct the commercial strains in Pleurotus spp., 81 strains in eight Pleurotus species were used. DNA fingerprinting using URP-PCR was conducted to determine the phylogenetic relationships among Pleurotus strains. DNA profiles of Pleurotus species obtained by twelve URP primers were analyzed for genetic similarity by NTSYS program. We could divide strains into ten clusters, in which three of them belong to P. ostreatus and the others to the different species, respectively. At the 76% similarity level, 70 P. ostreatus strains were distinguished into three clusters. Cluster I contained 35 strains and some of them showed almost 100% similarity, one strain closely related to Weonhyeong and six strains closely related to Wangheukpyeong. In cluster II, twenty-one out of 23 strains showed 100% to Suhan. Cluster III contained twelve strains, including six strains closely related to Chunchu-2. The results suggested that there are many same strains with different names in mushroom spawn market.

본 연구에 사용된 느타리버섯류는 느타리속 8종 81개 품종으로 형태적 분류가 어려운 점 외에도 출처가 불확실한 품종이 재배되는 경우가 많아 정확한 품종구분이 요구되고 있다. 이러한 문제점을 해결하고자 국내에서 재배 유통되고 있는 느타리버섯류 81개 품종을 DNA 핵산지문법을 이용하여 유연관계를 분석한 결과 10 그룹으로 나누어졌으며, 이 중 느타리종에 속하는 70품종은 3 그룹으로 나누어졌다. 또한 이 그룹내에서 국내 주요품종인 원형, 수한, 춘추2호, 왕흑평과 동일하거나 유사한 것으로 나타난 품종이 많았다. I 그룹에 속하는 35개 품종 중 원형품종과 동일하거나 구별성이 인정되지 않는 품종은 4개 품종이었고, 왕흑평과는 6개의 품종이 동일한 것으로 나타났다. II 그룹의 23개 품종 중에는 21개의 품종이 수한과 구별성이 인정되지 않았다. 또한 II 그룹의 12개 품종 중 춘추2호와 유사 한 품종들은 총 6개였다.

Keywords

References

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