Korean Journal of Medicinal Crop Science (한국약용작물학회지)

  • 제16권6호
  • /
  • Pages.402-408
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  • 2008
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Enhancement of Immune Activities of Natural Water-Soluble Sulforaphane by Nano Encapsulation Process

천연 수용성 설포라판의 나노입자화를 통한 면역 활성 증진

  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Han, Jae-Gun (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Oh, Sung-Ho (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Young-Beom (Jeong Woo Foods Co.) ;
  • Ko, Jung-Rim (Jeong Woo Foods Co.) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University)
  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 한재건 (강원대학교 BT특성화학부대학) ;
  • 정향숙 (강원대학교 BT특성화학부대학) ;
  • 오성호 (강원대학교 BT특성화학부대학) ;
  • 권민철 (강원대학교 BT특성화학부대학) ;
  • 최영범 (정우식품) ;
  • 고정림 (정우식품) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • 발행 : 2008.12.30
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초록

This study was performed to investigate improving immune activities of natural water-soluble sulforaphane extracted from Brassica oleracea var. italica by nano encapsulation process. The nanoparticles of the sulforaphane extracted with ultrasonification process at $60^{\circ}C$ promoted human B and T cell growth, about $7{\sim}35%$ compared to the control. The secretion of IL-6 and TNF-${\alpha}$ from T cells were also enhanced as $2.6{\times}10^{-4}pg/cell$ and $2.1{\times}10^{-4} pg/cell$, respectively, by the adding nano samples. NK cell activation was improved about 8%, compare to the control in adding cultured medium of T cell added nano samples. It was also found that sulforaphane extracted from B. oleracea var. italica had highly inhibitory activity on hyaluronidase as $IC_{50}$ about $200\;{\mu}g/m{\ell}$. It can be concluded that natural water-soluble sulforaphane samples by nano-encapsulation, each size is 200 nm, extracted from B. oleracea var. italica has high immune activities through higher efficiency of bio-activation than conventional extracts.

키워드

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