Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Asparagus cochinchinensis (Lour.) Merr. on the Stimulation of Osteoblast Differentiation and Inhibition of Osteoclast Generation

천문동 추출물에 의한 조골세포 분화 촉진 및 파골세포 생성 억제효과

  • 이승연 ((주)KMSI 부설 한국의과학연구소) ;
  • 김시나 ((주)KMSI 부설 한국의과학연구소) ;
  • 김종근 ((주)KMSI 부설 한국의과학연구소)
  • Published : 2008.01.31
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Abstract

Bone mass in adults decreases with age because of the imbalance between the rate of bone formation and resorption. We performed this study to investigate whether Asparagus cochinchinensis (Lour.) Merr. (ACAE) plays a role in osteoblasts differentiation and osteoclasts formation. Ethanol extract of ACAE showed increase in the differentiation and alkaline phosphatase activity of osteoblasts. Also, it decreased the number of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells (OCLs) and TRAP activity. Therefore, ACAE has the potential to prevent bone-related diseases such as osteoporosis by increasing the differentiation of osteoblasts and reducing both the number and activity of osteoclasts.

천문동 에탄올 추출물은 조골세포의 증식을 농도 및 시간 의존적으로 유의성 있게 증가시켰으며 조골세포 분화유도 및 석회화에 관여하는 염기성 인산분해효소 활성을 효과적으로 촉진시켰다. 또한 TRAP 양성인 다핵 파골세포수 및 TRAP 활성도를 감소시켰다. 결과적으로 천문동 에탄올 추출물은 조골세포의 활성 증가 및 파골세포의 활성 억제 등뼈의 대사에 영향을 미치므로 뼈의 대사와 관련된 치료제 및 골다공증 예방 건강 보조식품으로서 개발 가능성이 있으며, 이와 관련된 작용기전 및 활성성분의 분리, 정제에 대한 연구가 더욱 필요할 것으로 사료된다.

Keywords

References

  1. Kalervo V, Haibo Z, Mika M, Jussi MH. 2000. The cell biology of osteoclast function. J Cell Sci 113: 377-381
  2. Suda T, Nakamura I, Jimi B, Takahashi N. 1997. Regulation of osteoclast function. J Bone Mineral Res 12: 869-879 https://doi.org/10.1359/jbmr.1997.12.6.869
  3. Im GI, Quershi SA, Kenny J, Rubash HE, Shanbhag AS. 2004. Osteoblast proliferation and maturation by bisphosphonates. Biomaterials 25: 4105-4115 https://doi.org/10.1016/j.biomaterials.2003.11.024
  4. Cryer R, Bauer DC. 2002. Oral bisphosphonates and upper gastrointestinal tract problem: what is the evidence? Mayo Clin Proc 77: 1031-1043 https://doi.org/10.4065/77.10.1031
  5. Recker RP. 1993. Current theraphy for osteoporosis. J Clin Endocrinol Metab 76: 14-16 https://doi.org/10.1210/jc.76.1.14
  6. Kim SW, Kim HG, Lee BE, Hwand HH, Baek DH, Ko SY. 2006. Effects of mushroom, leurotus eryngii, extracts on bone metabolism. Clin Nutr 25: 166-170 https://doi.org/10.1016/j.clnu.2005.10.014
  7. Muhlbauer RC, Lozano A, Reinli A. 2002. Onion and a mixture of vegetables, salads, and herbs affect bone resorption in the rat by a mechanism independent of their base excess. J Bone Mineral Res 17: 1230-1236 https://doi.org/10.1359/jbmr.2002.17.7.1230
  8. Ochiuto F, Pasquale RD, Guglielmo G, Palumbo DR, Zangla G, Samperi S, Renzo A, Circosta C. 2007. Effects of phytoestrogenic isoflavones from red clover (Trifolium pretense L.) on experimental osteoporosis. Phytother Res 21: 130-134 https://doi.org/10.1002/ptr.2037
  9. Lee JW, Lee IS. 2004. Effects of Rubus coreanus Miquel extracts on the activity and differentiation of MC3T3-E1 osteoblastic cell. J Life Sci 14: 967-974 https://doi.org/10.1016/0024-3205(74)90086-1
  10. Park JH, Lee JW, Kim HJ, Lee IS. 2005. Effects of Solidago virga-aurea var. gigantea Miq. root extracts on the activity and differentiation of MC3T3-E1 osteoblastic cell. J Kor Soc Food Sci Nutr 34: 929-936 https://doi.org/10.3746/jkfn.2005.34.7.929
  11. 최옥자. 1991. 약초의 성분과 이용. 일월서각, 서울. p 672
  12. Koo HN, Jeong HJ, Choi JY, Choi SD, Choi TJ, Cheon YS, Kim KS, Kang BK, Park ST, Chang CH, Kim CH, Lee YM, Kim HM, An NH, Kim JJ. 2000. Inhibition of tumor necrosis factor-$\alpha$-induced apoptosis by Asparagus cochinchinensis in Hep G2 cells. J Ethnopharm 73: 137-143 https://doi.org/10.1016/S0378-8741(00)00287-7
  13. Kim H, Lee E, Lim T, Jung J, Lyu Y. 1998. Inhibitory effect of Asparagus cochinchinensis on tumor necrosis factor-alpha secretion from astrocytes. Inter J Immunopharm 20: 153-162 https://doi.org/10.1016/S0192-0561(98)00022-8
  14. Mosmann T. 1983. Rapid colorimetric assay for the cellular growth and survival; application to proliferation and cytotoxic assay. J Immun Methods 65: 56-58 https://doi.org/10.1016/0022-1759(83)90303-4
  15. Boyce BF, Xing L. 2006. Osteoclast, no longer osteoblast slaves. Nature Med 12: 1356-1358 https://doi.org/10.1038/nm1206-1356
  16. Suda T, Takahashi N, Martin TJ. 1992. Modulation of osteoclast differentiation. Endo Reviews 13: 66-80
  17. Laurence D, Maylis D, Philippe J, Dominique H. 2007. Embryonic stem cells: new tool to study osteoblast and osteoclast differentiation. Stem Cells 25: 544-552 https://doi.org/10.1634/stemcells.2006-0395
  18. Karst M, Gorny G, Galvin RJ, Oursler MJ. 2004. Roles of stromal cell RANKL, OPG, and M-CSF expression in biphasic TGF-$\beta$ regulation of osteoclast differentiation. J Cell Physiol 200: 99-106 https://doi.org/10.1002/jcp.20036
  19. Vaananen HK, Horton M. 1995. The osteoclast clear zone is a specialized cell extracellular matrix adhesion structure. J Cell Sci 108: 2729-2732

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