Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Effects of Exposure Period on the Developmental Toxicity of 2-Bromopropane in Sprague-Dawley Rats

  • Shin, In-Sik (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Jong-Chan (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Kang-Hyeon (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Ahn, Tai-Hwan (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Bae, Chun-Sik (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Moon, Chang-Jong (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-Ho (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Dong-Ho (Animal Medical Center, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (Animal Medical Center, College of Veterinary Medicine, Chonnam National University)
  • Published : 2008.12.01
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Abstract

Recently we reported that 2-bromopropane (2-BP) has maternal toxicity, embryotoxicity, and teratogenicity in Sprague-Dawley rats. The aims of this study are to examine the potential effects of 2-BP administration on pregnant dams and embryo-fetal development, and to investigate the effects of metabolic activation induced by phenobarbital (PB) on developmental toxicities of 2-BP. Pregnant rats received 1000 mg/kg/day subcutaneous 2-BP injections on gestational days (GD) 6 through 10 (Group II and Group IIII) or 11 through 15 (Group IV). Pregnant rats in Group III received an intraperitoneal PB injection once daily at 80 mg/kg/day on GD 3 through 5 for induction of the liver metabolic enzyme system. Control rats received vehicle injections only on GD 6 through 15. All dams underwent caesarean sections on GD 20 and their fetuses were examined for external, visceral, and skeletal abnormalities. Significant adverse effects on pregnant dams and embryo-fetal development were observed in all the treatment groups, and the maternal and embryo-fetal effects of 2-BP observed in Group II were higher than those seen in Group IV. Conversely, maternal and embryo-fetal developmental toxicities observed in Group III were comparable to those seen in Group II. These results suggest that the potential effects of 2-BP on pregnant dams and embryo-fetal development are more likely in the first half of organogenesis (days $6{\sim}10$ of pregnancy) than in the second half and that the metabolic activation induced by PB pre-treatment did not modify the developmental toxic effects of 2-BP in rats.

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References

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