Biomolecules & Therapeutics

  • 제16권4호
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  • Pages.403-409
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  • 2008
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Suppressive Effect of a Carbohydrate Fraction from Eclipta prostrata on the Apoptosis of the Mouse Splenocytes in Culture

  • Bae, Eun-Kyong (School of Biological Sciences and Bio-health Products Research Center, Inje University) ;
  • Kim, Na-Ri (School of Biological Sciences and Bio-health Products Research Center, Inje University) ;
  • Yun, Mi-Jung (School of Biological Sciences and Bio-health Products Research Center, Inje University) ;
  • Youn, Hyong-Chol (School of Biological Sciences, Seoul National University) ;
  • Youn, Kyung-Joon (School of Biological Sciences, Seoul National University) ;
  • Lee, Kang-Ro (Natural Products Laboratory, College of Pharmacy, Sungkyunkwan University) ;
  • Youn, Hyun-Joo (School of Biological Sciences and Bio-health Products Research Center, Inje University)
  • 발행 : 2008.12.31
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초록

Eclipta prostrata grows abundantly in the tropical and the sub-tropical parts of the world including most part of the Korean Peninsula. The plant has been traditionally used for the treatment of a number of inflammatory diseases including hepatitis and enteritis but the nature of its immuno-modulating activity needs more studies. In this study, water-soluble sugar-containing fractions were purified from the herb and their effects on the culture of mouse splenocytes were examined. One of the fractions significantly suppressed apoptosis of the splenocytes in culture, which involves the gene expression regulation of a number of cytokines and cytokine receptors including MIP1-$\beta$. This study could explain an immunological activity of Eclipta prostrata and would lead to identify an immuno-active compound from the plant.

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참고문헌

  1. Akbar, A. N., Borthwick, N. J., Wickremasinghe, R. G., Panayoitidis, P., Pilling, D., Bofill, M., Krajewski, S., Reed, J. C. and Salmon, M. (1996). Interleukin-2 receptor common gammachain signaling cytokines regulate activated T cell apoptosis in response to growth factor withdrawal: selective induction of anti-apoptotic (bcl-2, bcl-xL) but not pro-apoptotic (bax, bclxS) gene expression. Eur. J. Immunol. 26, 294-299 https://doi.org/10.1002/eji.1830260204
  2. Alimonti, J. B., Ball, T. B. and Fowke, K. R. (2003). Mechanisms of CD4+ T lymphocyte cell death in human immunodeficiency virus infection and AIDS. J. Gen. Virology, 84, 1649–1661 https://doi.org/10.1099/vir.0.19110-0
  3. Amara, A., Le Gall, S., Schwartz, O., Salamero, J., Montes, M., Loetscher, P., Baggiolini, M., Virelizier, J.-L. and Arenzana-Seisdedos, F. (1997). HIV Coreceptor downregulation as antiviral principle: SDF-1-dependent internalization of the chemokine receptor CXCR4 contributes to inhibition of HIV replication. J. Exp. Med. 186, 139-146 https://doi.org/10.1084/jem.186.1.139
  4. Annumziata, C. M., Safiran, Y. J, Irving, S. G., Kasid, U. N. and Cossman, J. (2000). Hodgkin diseases: pharmacologic intervention of CD40-NF kappa B pathway by a protease inhibitor. Blood 96, 2841-2848
  5. Choi, K. S., Eom, Y. W., Kang, Y., Ha, M. J., Rhee, H., Yoon, J. W. and Kim, S. J. (1999). Cdc2 and Cdk2 kinase activated by transforming growth factor-beta1 trigger apoptosis through the phosphorylation of retinoblastoma protein in FaO hepatoma cells. J. Biol Chem. 274, 31775-31783 https://doi.org/10.1074/jbc.274.45.31775
  6. Bendjeddou, D., Lalaoui, K. and Stta, D. (2003). Immunostimulating activity of the hot water-soluble polysaccharide extracts of Anacycyclus pyrethrum, Alpinia galanga and Citrullus colocynthis. J. Ethnopharmacol. 88, 155-160 https://doi.org/10.1016/S0378-8741(03)00226-5
  7. Fedele, G., Celestino, I., Spensieri, F., Frasca, L., Nasso, M., Watanabe, M., Remoli, M. E., Coccia, E. M., Altieri, F. and Ausiello, C. M. (2007). Lipooligosaccharide from Bordetella pertussis induces mature human monocyte-derived dendritic cells and drives a Th2 biased response. Microbes Infect. 9, 855-863 https://doi.org/10.1016/j.micinf.2007.03.002
  8. Hahm, K. B., Kim, J. H., You, B. M., Kim, Y. S., Cho, S. W., Yim, H., Ahn, B. O. and Kim, W. B. (1998). Induction of apoptosis with an extract of Artemisia asiatica attenuates the severity of cerulein-induced pancreatitis in rats. Pancreas 17, 153-157 https://doi.org/10.1097/00006676-199808000-00007
  9. Han. Y., Xia, C., Cheng, X., Xiang, R., Liu, H., Yan, Q. and Xu, D. (1998). Preliminary studies on chemical constituents and pharmacological action of Eclipta prostrata L. Materia Medica. 23, 680-682
  10. Ishizaki, Y., Cheng, L., Mudge, A. W. and Raff, M. C. (1995). Programmed cell death by default in embryonic cells, fibroblasts, and cancer cells. Mol. Biol. Cell. 6, 1443-1458 https://doi.org/10.1091/mbc.6.11.1443
  11. Hwang J., Chung, H., Bae, E., Lee, A., Ji H., Park, D., Jung, H. J., Cho, C. W, Choi, H., Lee, D., Lee, K. and Youn, H. (2003). The Polysaccharide Fraction AIP1 from Artemisia iwayomogi Suppresses Apoptotic Death of the Mouse Spleen Cell in Culture. Arch. Parm. Res. 4, 294-300
  12. Kamin-Lewis, R., Abdelwahab, S. F., Trang, C., Baker, A., DeVico, A. L., Gallo, R. C. and Lewis, G. K. (2001). Perforinlow memory CD8+ cells are the predominant T cells in normal humans that synthesize the beta -chemokine macrophage inflammatory protein-1beta. Proc. Natl. Acad. Sci. USA. 98, 9283-9288 https://doi.org/10.1073/pnas.161298998
  13. Katayose, Y., Kim, M., Rakkar, A. N., Li, Z., Cowan, K. H. and Seth, P. (1997). Promoting apoptosis: a novel activity associated with the cyclin-dependent kinase inhibitor p27. Cancer Res. 57, 5441-5445
  14. Kobari, M., Yang, Z., Gong, D., Heissmeyer, V., Zhu, H., Jung, Y. K., Angelica, M., Gakidis, M., Rao, A., Sekine, T., Ikegami, F., Yuan, C. and Yuan, J. (2004). Wedelolactone suppress LPS- induced caspase-11 expression by directly inhibiting the IKK complex. J. Cell. Death Differ. 11, 123-130 https://doi.org/10.1038/sj.cdd.4401325
  15. Kroemer, G., Bosca, L., Zamzami, N., Merchetti, P., Hortelano, S. and Martinez-A, C. (1997). Detection of apoptosis and apoptosis-associated alterations. In: Lefkovits I. Immunology Methods Manual, Academic Press, San Diego, pp. 1111-1121
  16. Kumar, A., Commane, M., Flickinger, T. W., Horvath, C. M. and Stark, G. R. (1997). Defective TNF-alpha-induced apoptosis in STAT1-null cells due to low constitutive levels of caspases. Science 278, 1630-1632 https://doi.org/10.1126/science.278.5343.1630
  17. Kumari, C. S., Govindasamy, S. and Sukumar, E. (2006). Lipid lowering activity of Eclipta prostrata in experimental hyperlipidemia. J. Ethnopharmacol. 24, 332-335 https://doi.org/10.1016/j.jep.2005.10.031
  18. Kusumi, M., Yamashita, T., Fujii, T., Nagamatsu, T., Kozuma, S. and Taketani, Y. (2006). Expression patterns of lectin-like natural killer receptors, inhibitory CD94/NKG2A, and activating CD94/NKG2C on decidual CD56bright natural killer cells differ from those on peripheral CD56dim natural killer cells. J. Reprod. Immunol. 70, 33-42 https://doi.org/10.1016/j.jri.2005.12.008
  19. Lindsten, T., Ross, A. J., King, A., Zong, W., Rathmell, J. C., Shiels, H. A., Ulrich, E., Waymire, k. G., Mahar, P., Frauwirth, K., Chen, Y., Wei, M., Eng, V. M., Adelman, D. M., Simon, M. C., Ma, A., Golden, J. A., Evan, G., Korsmeyer, S. J., MacGregor G. R. and Thompson, C. B. (2000). The combined functions of proapoptotic Bcl-2 family members Bak and Bax are essential for normal development of multiple tissues. Molecular Cell 6, 1389-1399 https://doi.org/10.1016/S1097-2765(00)00136-2
  20. Luo, X., Budihardjo, I., Zou, H., Slaughter, C. and Wang, X. (1998). Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94, 481-490 https://doi.org/10.1016/S0092-8674(00)81589-5
  21. Modi, W. S., Bergeron, J. and Sanford, M. (2001). The human MIP-1-beta chemokine is encoded by two paralogous genes, ACT-2 and LAG-1. Immunogenetics 53, 543-549 https://doi.org/10.1007/s002510100366
  22. Mores, W. B., do Nascimento, M. C., Parente, J. P., da Silvia, M. H., Melo, P. A. and Suarez-Kurtz, G. (1998). Neutralization of lethal and myotoxic activitis of South American rattle snake venom by extracts and constituents of the plant Eclipta prostrata (Asteraceae). Toxicon 27, 1003-1009 https://doi.org/10.1016/0041-0101(89)90151-7
  23. Srivastava, R. and Kulshreshtha, K. (1989). Bioactive polysaccharides from plants. Phytochemistry 28, 2877-2883 https://doi.org/10.1016/0031-9422(89)80245-6
  24. Sullivan, B. A. and Kronenberg, M. (2007). TCR-mediated recognition of glycolipid CD1 complexes. Curr. Top Microbiol. Immunol. 314, 165-193 https://doi.org/10.1007/978-3-540-69511-0_7
  25. Taylor, K. (1995). A modification of the phenol sulfuric acid method of total sugar determination. Appl. Biochem. Biotechnol. 53, 207-214 https://doi.org/10.1007/BF02783496
  26. Tewtrakul, S., Subhadhirasakul, S., Cheenpracha, S. and Karalai, C. (2007). HIV-1 protease and HIV-1 integrase inhibitory substances from Eclipta prostrata. Phytother. Res. 21, 1092-1095 https://doi.org/10.1002/ptr.2252
  27. Van Vliet, S., Dunnen, J., Gringhuis, S., Geijtenbeek, T. and van Kooyk, Y. (2007) Innate signaling and regulation of dendritic cell immunity. Curr. Opin. Immunol. 19, 435-440 https://doi.org/10.1016/j.coi.2007.05.006
  28. Wajant, H. (2002) The Fas signaling pathway: more than a paradigm. Science 296, 1635-1636 https://doi.org/10.1126/science.1071553
  29. Winnicka, K., Bielawski, K. and Bielawska, A. (2006) Cardiac glycosides in cancer research and cancer therapy. Acta. Polon Pharm. 2, 109-115
  30. Winnicka, K., Bielawski, K., Bielawska, A. and Miltyk, W. (2007) Apoptosis-Mediated Cytotoxicity of Ouabain, Digoxin and Proscillaridin A in the Estrogen Independent MDA-MB-231 Breast Cancer Cells. Arch. Pharm. Res. 30, 1216-1224 https://doi.org/10.1007/BF02980262

피인용 문헌

  1. Sulphur compounds from the aerial parts of Eclipta prostrata vol.38, pp.6, 2010, https://doi.org/10.1016/j.bse.2010.12.024