KSBB Journal

  • 제23권4호
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  • Pages.291-296
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  • 2008
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

인체 양막의 골형성유도능 평가

Evaluation of Osteoinduction Efficacy of Human Amniotic Membrane

  • 한정욱 (중앙대학교 의과대학 병리학교실) ;
  • 서영권 (동국대학교 생명화학공학과) ;
  • 박정극 (동국대학교 생명화학공학과) ;
  • 송계용 (중앙대학교 의과대학 병리학교실)
  • Han, Jung-Wook (Department of Pathology, Chung-Ang University) ;
  • Seo, Young-Kwon (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Park, Jung-Keug (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Song, Kye-Yong (Department of Pathology, Chung-Ang University)
  • 발행 : 2008.08.29
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초록

양막은 다양한 생체재료로 이용되어 왔으며 생체적합성과 환부의 치료효능이 우수한 것으로 알려져 있다. 본 연구에서는 양막을 갈아 양막스폰지를 제조하고 중간엽줄기세포를 배양한 뒤 누드마우스이식을 통하여 콜라젠스폰지와 비교하여 골형성유도능에 대한 연구를 실시하였다. 연구결과 세포의 부착과 증식면에서는 두 시료가 유사하였으나, 체내에 이식한 결과 양막스포지군에서 좀 더 많은 콜라젠 분비와 칼슘이 침착되었음을 확인하였다. 그리고 면역화학염색 결과 골형성 시 필요한 오스테오칼신과 오스테오넥틴이 좀 더 발현된 것을 관찰할 수 있었다. 따라서 양막은 골형성유도를 향상시킬 수 있는 가능성이 있음을 알 수 있었다.

Amniotic membrane (AM) has been used in various medical application such as biomaterials and it has a biocompatibility and wound healing effects. In this studies, we made AM sponge that was homogenized with AM and then lyophilized. And osteoinduction efficacy of AM sponge was evaluated with collagen sponge by mesenchymal stem cell culture and implantation in nude mouse. As a result of this study, adhesion and proliferation of MSC cells on AM sponge and collagen sponge were not different, but AM sponge was more superior to collagen sponge for induction of collagen secretion and calcium adhesion in matrix in vivo. Besides, AM sponges were more positive stained than collagen sponge about osteocalcin and osteonectin. As a results of this study, there is possibility of doing that AM could increase osteoinduction.

키워드

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