Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Characterization of Interaction Between Porcine Reproductive and Respiratory Syndrome Virus and Porcine Dendritic Cells

  • Park, Jie-Yeun (Division of Preventive Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Hyun-Soo (Division of Preventive Medicine, College of Veterinary Medicine, Chungnam National University) ;
  • Seo, Sang-Heui (Division of Preventive Medicine, College of Veterinary Medicine, Chungnam National University)
  • Published : 2008.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

The porcine reproductive and respiratory syndrome Virus (PRRSV) is an infectious disease that causes abortions and respiratory disorders in swine. In this study, the interaction between PRRSV and porcine dendritic cells generated from $CD14^{+}$ monocytes in the presence of GM-CSF and IL-4 was examined. As a result, it was shown that immature and mature dendritic cells can be productively infected with PRRSV. When the expression of surface MHC molecules on infected dendritic cells was determined, MHC classes I and II were found to be downregulated when compared with un infected dendritic cells. With the exception of the IL-4 and IFN-$\gamma$ cytokines, the induction of the IL-10, IL-12, and TNF-$\alpha$ cytokines all increased in dendritic cells infected with PRRSV. A mixed lymphocyte reaction showed that peripheral blood mononuclear cells cocultured with PRRSV-infected dendritic cells were less stimulated than peripheral blood mononuclear cells cocultured with dendritic cells treated with PBS, LPS, or UV-inactivated PRRSV. Therefore, these results suggest that PRRSV would appear to modulate the immune stimulatory function of porcine dendritic cells.

Keywords

References

  1. Banchereau, J. and R. M. Steinman. 1998. Dendritic cells and the control of immunity. Nature 392: 245-252 https://doi.org/10.1038/32588
  2. Banchereau, J., F. Briere, C. Caux, J. Davoust, S. Lebecque, Y. J. Liu, B. Pulendran, and K. Palucka. 2000. Immunobiology of dendritic cells. Annu. Rev. Immunol. 18: 767-811 https://doi.org/10.1146/annurev.immunol.18.1.767
  3. Bender, A., L. K. Bui, M. A. Feldman, M. Larsson, and N. Bhardwaj. 1995. Inactivated influenza virus, when presented on dendritic cells, elicits human $CD8^+$ cytolytic T cell responses. J. Exp. Med. 182: 1663-1671 https://doi.org/10.1084/jem.182.6.1663
  4. Benfield, D. A., E. Nelson, J. E. Collins, L. Harris, S. M. Goyal, D. Robison, et al. 1992. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J. Vet. Diagn. Invest. 4: 127-133 https://doi.org/10.1177/104063879200400202
  5. Bhardwaj, N., A. Bender, N. Gonzalez, L. K. Bui, M. C. Garrett, and R. M. Steinman. 1994. Influenza virus-infected dendritic cells stimulate strong proliferative and cytolytic responses from human $CD8^+$ T cells. J. Clin. Invest. 94: 797-807 https://doi.org/10.1172/JCI117399
  6. Cavanagh, D. 1997. Nidovirales: A new order comprising Coronaviridae and Arteriviridae. Arch. Virol. 142: 629-633
  7. Cella, M., M. Salio, Y. Sakakibara, H. Langen, I. Julkunen, and A. Lanzavecchia. 1999. Maturation, activation, and protection of dendritic cells induced by double-stranded RNA. J. Exp. Med. 189: 821-829 https://doi.org/10.1084/jem.189.5.821
  8. Duan, X., H. J. Nauwynck, and M. B. Pensaert. 1997. Effects of origin and state of differentiation and activation of monocytes/ macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV). Arch. Virol. 142: 2483-2497 https://doi.org/10.1007/s007050050256
  9. Gagnon, C. A. and S. Dea. 1998. Differentiation between porcine reproductive and respiratory syndrome virus isolates by restriction fragment length polymorphism of their ORFs 6 and 7 genes. Can. J. Vet. Res. 62: 110-116
  10. Grosjean, I., C. Caux, C. Bella, I. Berger, F. Wild, J. Banchereau, and D. Kaiserlian. 1997. Measles virus infects human dendritic cells and blocks their allostimulatory properties for $CD4^+$ T cells. J. Exp. Med. 186: 801-812 https://doi.org/10.1084/jem.186.6.801
  11. Hahn, G., R. Jores, and E. S. Mocarski. 1998. Cytomegalovirus remains latent in a common precursor of dendritic and myeloid cells. Proc. Natl. Acad. Sci. USA 95: 3937-3942
  12. Jo, S. K., H. S. Kim, S. W. Cho, and S. H. Seo. 2007. Genetic and antigenic characterization of swine H1N2 influenza viruses isolated from Korean pigs. J. Microbiol. Biotechnol. 17: 868-872
  13. Keffaber, K. 1989. Reproductive failure of unknown aetiology. AASP Newslett. 1: 1-10
  14. Kim, H. S., J. Kwang, I. J. Yoon, H. S. Joo, and M. L. Frey. 1993. Enhanced replication of porcine respiratory and reproductive syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch. Virol. 133: 477-483 https://doi.org/10.1007/BF01313785
  15. Kim, Y. and D. Jeoung, 2008. Role of CAGE, a novel cancer/testis antigen, in various cellular processes, including tumorigenesis, cytolytic T lymphocyte induction, and cell motility. J. Microbiol. Biotechnol. 18: 600-610
  16. Klagge, I. M. and S. Schneider-Schaulies. 1999. Virus interactions with dendritic cells. J. Gen. Virol. 80: 823-833 https://doi.org/10.1099/0022-1317-80-4-823
  17. Lane, P. J. and T. Brocker. 1999. Developmental regulation of dendritic cell function. Curr. Opin. Immunol. 11: 308-313 https://doi.org/10.1016/S0952-7915(99)80049-1
  18. Langhoff, E., E. F. Terwilliger, H. J. Bos, K. H. Kalland, M. C. Poznansky, O. M. Bacon, and W. A. Haseltine. 1991. Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures. Proc. Natl. Acad. Sci. USA 88: 7998-8002
  19. MacDonald, G. H. and R. E. Johnston. 2000. Role of dendritic cell targeting in Venezuelan equine encephalitis virus pathogenesis. J. Virol. 74: 914-922 https://doi.org/10.1128/JVI.74.2.914-922.2000
  20. Meulenberg, J. J., M. M. Hulst, E. J. de Meijer, P. L. Moonen, A. den Besten, E. P. de Kluyver, G. Wensvoort, and R. J. Moormann. 1993. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192: 62-72 https://doi.org/10.1006/viro.1993.1008
  21. Molitor, T. W., J. Xiao, and C. Choi. 1996. PRRS virus infection of macrophages: Regulation by maturation and activation state, pp. 563-569. In: Proceedings of the 27th Annual Meeting of the American Association of Swine Practitioners. Nashville, Tennessee
  22. Murtaugh, M. P., Z. Xiao, and F. A. Zuckermann. 2002. Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol. 15: 533-547 https://doi.org/10.1089/088282402320914485
  23. Nelsen, C. J., M. P. Murtaugh, and K. S. Faaberg. 1999. Porcine reproductive and respiratory syndrome virus comparison: Divergent evolution on two continents. J. Virol. 73: 270-280
  24. Oldstone, M. B. 1998. Viral persistence: Mechanisms and consequences. Curr. Opin. Microbiol. 1: 436-441 https://doi.org/10.1016/S1369-5274(98)80062-3
  25. Palucka, K. A., N. Taquet, F. Sanchez-Chapuis, and J. C. Gluckman. 1998. Dendritic cells as the terminal stage of monocyte differentiation. J. Immunol. 160: 4587-4595
  26. Rossow, K.D. 1998. Porcine reproductive and respiratory syndrome. Vet. Pathol. 35: 1-20 https://doi.org/10.1177/030098589803500101
  27. Sur, J. H., A. R. Doster, J. S. Christian, J. A. Galeota, R. W. Wills, J. J. Zimmerman, and F. A. Osorio. 1997. Porcine respiratory and reproductive syndrome virus replicates in testicular germ cells, alters spermatogenesis, and induces germ cell death by apoptosis. J. Virol. 71: 9170-9179
  28. Thanawongnuwech, R., G. B. Brown, P. G. halbur, R. L. Royer, and B. J. Thackerr. 2000. Pathogenesis of porcine reproductive and respiratory syndrome virus-induced increase in susceptibility to Streptococcus suis infection. Vet Pathol. 37: 143-152 https://doi.org/10.1354/vp.37-2-143
  29. Wensvoort, G., C. Terpstra, J. M. Pol, E. A. ter Laak, M. Bloemraad, E. P. de Kluyver, et al. 1991. Mystery swine disease in the Netherlands: The isolation of Lelystad virus. Vet. Quart. 13: 121-130 https://doi.org/10.1080/01652176.1991.9694296
  30. Will, R. W., J. J. Zimmerman, S. L. Yoon, M. J. Swenson, M. J. McGinley, H. T Hill, K. B. Platt, J. Christopher-Hennings, and E. A. Nelson. 1997. Porcine reproductive and respiratory syndrome virus: Apersistent infection. Vet. Microbiol. 55: 231-240 https://doi.org/10.1016/S0378-1135(96)01337-5
  31. Wu, W. H., Y. Fang, R. Farwell, M. Steffen-Bien, R. R. Rowland, J. Christopher-Hennings, and E. A. Nelson. 2001. A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by orf2b. Virology 287: 183-191 https://doi.org/10.1006/viro.2001.1034
  32. Yoon, K. J., L. L. Wu, J. J. Zimmerman, H. T. Hill, and K. B. Platt. 1996. Antibody-dependent enhancement (ADE) of porcine reproductive and respiratory syndrome virus (PRRSV) infection in pigs. Viral Immunol. 9: 51-63 https://doi.org/10.1089/vim.1996.9.51