Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of an ISR-Related Metabolite Produced by Pseudomonas chlororaphis O6 against the Wildfire Pathogen Pseudomonas syringae pv. tabaci in Tobacco

  • Park, Myung-Ryeol (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Young-Cheol (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Park, Ju-Yeon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Han, Song-Hee (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Sun-Woo (Department of Chemistry, College of Natural Science, Chonnam National University) ;
  • Kim, In-Seon (Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, College of Agriculture and Life Sciences, Chonnam National University)
  • Published : 2008.10.31
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Abstract

Pseudomonas chlororaphis O6 exhibits induced systemic resistance (ISR) against P. syringae pv. tabaci in tobacco. To identify one of the ISR metabolites, O6 cultures were extracted with organic solvents, and the organic extracts were subjected to column chromatography followed by spectroscopy analyses. The ISR bioassay-guided fractionation was carried out for isolation of the metabolite. High-resolution mass spectrometric analysis of the metabolite found $C_{9}H_{9}O_{3}N$ with an exact mass of 179.0582. LC/MS analysis in positive mode showed an $(M+H)^{+}$ peak at m/z 180. Nuclear magnetic resonance ($^{1}H,\;^{13}C$) analyses identified all protons and carbons of the metabolite. Based on the spectroscopy data, the metabolite was identified as 4-(aminocarbonyl) phenylacetate (4-ACPA). 4-ACPA applied at 68.0 mM exhibited ISR activity at a level similar to 1.0 mM salicylic acid. This is the first report to identify an ISR metabolite produced by P. chlororaphis O6 against the wildfire pathogen P. syringae pv. tabaci in tobacco.

Keywords

References

  1. Audenaert, K., T. Pattery, P. Cornelis, and M. Hofte. 2002. Induction of systemic resistance to Botrytis cinerea in tomato by Pseudomonas aeruginosa 7NSK2: Role of salicylic acid, pyochelin, and pyocyanin. Mol. Plant Microbe Interact. 15: 1147-1156 https://doi.org/10.1094/MPMI.2002.15.11.1147
  2. De Meyer, G. and M. Hofte. 1997. Salicylic acid produced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 induces resistance to leaf infection by Botrytis cinerea on bean. Phytopathology 87: 588-593 https://doi.org/10.1094/PHYTO.1997.87.6.588
  3. Delaney, T. P., S. Uknes, B. Vernooij, L. Friedrich, K. Weymann, D. Negrotto, et al. 1994. A central role of salicylic acid in plant disease resistance. Science 266: 1247-1250 https://doi.org/10.1126/science.266.5188.1247
  4. Han, S. H., S. J. Lee, J. H. Moon, K. H. Park, K. Y. Yang, B. H. Cho, et al. 2006. GacS-dependent production of 2R,3R-butanediol by Pseudomonas chlororaphis O6 is a major determinant for eliciting systemic resistance against Erwinia carotovora but not against Pseudomonas syringae pv. tabaci in tobacco. Mol. Plant Microbe Interact. 19: 924-930 https://doi.org/10.1094/MPMI-19-0924
  5. Han, S. H., A. J. Anderson, K. Y. Yang, B. H. Cho, K. Y. Kim, M. C. Lee, Y. H. Kim, and Y. C. Kim. 2006. Multiple determinants influence root colonization and induction of induced systemic resistance by Pseudomonas chlororaphis O6. Mol. Plant Pathol. 7: 463-472 https://doi.org/10.1111/j.1364-3703.2006.00352.x
  6. Heil, M. and R. M. Bostock. 2002. Induced systemic resistance (ISR) against pathogens in the context of induced plant defences. Ann. Bot. (Lond) 89: 503-512 https://doi.org/10.1093/aob/mcf076
  7. Kang, B. R., S. H. Han, R. E. Zdor, A. J. Anderson, M. Spencer, K. Y. Yang, et al. 2007. Inhibition of seed germination and induction of systemic disease resistance by Pseudomonas chlororaphis O6 requires phenazine production regulated by the global regulator, GacS. J. Microbiol. Biotechnol. 17: 586-593
  8. Kim, H. J., H. S. Nam, A. J. Anderson, K. Y. Yang, B. H. Cho, and Y. C. Kim. 2007. Mutation in the edd gene encoding the 6-phosphogluconate dehydratase of Pseudomonas chlororaphis O6 impairs root colonization and is correlated with reduced induction of systemic resistance. Lett. Appl. Microbiol. 44: 56-61 https://doi.org/10.1111/j.1472-765X.2006.02029.x
  9. Murphy, A. M., L. J. Holcombe, and J. P. Carr. 2000. Characteristics of salicylic acid-induced delay in disease caused by a necrotrophic fungal pathogen in tobacco. Physiol. Mol. Plant Pathol. 57: 47-54 https://doi.org/10.1006/pmpp.2000.0280
  10. Nam, H. S., A. J. Anderson, K. Y. Yang, B. H. Cho, and Y. C. Kim. 2006. The dctA gene of Pseudomonas chlororaphis O6 is under RpoN control and is required for effective root colonization and induction of systemic resistance. FEMS Microbiol. Lett. 256: 98-104 https://doi.org/10.1111/j.1574-6968.2006.00092.x
  11. Ongena, M., E. Jourdan, M. Schafer, C. Kech, H. Budzikiewicz, A. Luxen, and P. Thonart. 2005. Isolation of an N-alkylated benzylamine derivative from Pseudomonas putida BTP1 as elicitor of induced systemic resistance in bean. Mol. Plant Microbe Interact. 18: 562-569 https://doi.org/10.1094/MPMI-18-0562
  12. Oostendorp, M., W. Kunz, B. Dietrich, and T. Staub. 2001. Induced disease resistance in plants by chemicals. Eur. J. Plant Pathol. 107: 19-28 https://doi.org/10.1023/A:1008760518772
  13. Spencer, M., C.-M. Ryu, K.-Y. Yang, Y. C. Kim, J. W. Kloepper, and A. J. Anderson. 2003. Induced defence in tobacco by Pseudomonas chlororaphis strain O6 involves at least the ethylene pathway. Physiol. Mol. Plant Pathol. 63: 27-34 https://doi.org/10.1016/j.pmpp.2003.09.002