Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Characteristics of Agronomy Traits to Transgenic Rice Selected by Molecular Breeding Method

분자육종기법에 의해 선발된 형질전환 벼 계통의 작물학적 특성

  • Lee, Hyun-Suk (School of Applied Biosciences, Kyungpook National University) ;
  • Kang, Hyun-Goo (Department of Plant Resources, College of Life Science and Natural Resources, Kyungpook National University) ;
  • Park, Young-Hie (School of Applied Biosciences, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Chang-Kil (Department of Environmental Horticulture, College of Life Science and Natural Resources, Kyungpook National University) ;
  • Han, Jeung-Sul (National Institute of Hortcultural and Meicinal Crop, Rural Development Administration) ;
  • Sohn, Jae-Keun (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Kyung-Min (Department of Environmental Horticulture, College of Life Science and Natural Resources, Kyungpook National University) ;
  • Park, Gyu-Hwan (Department of Plant Resources, College of Life Science and Natural Resources, Kyungpook National University)
  • 이현숙 (경북대학교 응용생명과학부) ;
  • 강현구 (경북대학교 식물자원학과) ;
  • 박영희 (경북대학교 응용생명과학부) ;
  • 정희영 (경북대학교 응용생명과학부) ;
  • 김창길 (경북대학교 환경원예학과) ;
  • 한증술 (국립원예특작과학원) ;
  • 손재근 (경북대학교 응용생명과학부) ;
  • 김경민 (경북대학교 환경원예학과) ;
  • 박규환 (경북대학교 식물자원학과)
  • Published : 2008.10.31
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Abstract

This study was carried out to develop new cultivars using the $T_5$ generation of transformed rice by PCR analysis with DNA marker in each generation $(from\;T_3\;to\;T_5)$. In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants $(T_5)$ were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.

Arabidopsis thaliana $Ca^{2+}/H^+$ 수송체 유전자인 CAX 1(cation exchanger 1)이 도입된 벼 형질전환체에 대한 연구결과를 요약하면 다음과 같다. Agrobacterium 이용 형질전환 CAX 1 벼 $T_5$ 세대를 대상으로 CAX 1밴드가 도입되었음을 확인하였다. 농업적인 특징인 수장, 수수 및 간장은 모품종(일품)과 유사하였으며, 종자의 칼슘 함량도 높았다. 미립의 크기와 천립중은 모품종보다 약간 크나 거의 일치하고, 저온저항성과 도열병에 대한 내병성이 나타났다. $T_5$ 세대에서 형질이 안정적으로 발현되면서 모품종과 유사하고 농업적 형질이 우수한 3 계통을 선발하였다. 앞으로 $T_6$ 세대에 대한 특성조사를 거쳐 $Ca^{2+}$ 함량이 안정적으로 높게 유지되는 계통을 선발하여 세대육성하면 칼슘과 관련된 유전자의 내재해성 작물의 기초자료 이용되어 질 수 있을 것으로 사료된다.

Keywords

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