Parasites, Hosts and Diseases

  • 제46권3호
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  • Pages.145-151
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  • 2008
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Suppressed Production of Pro-inflammatory Cytokines by LPS-Activated Macrophages after Treatment with Toxoplasma gondii Lysate

  • Lee, Eun-Jung (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Heo, Yoo-Mi (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Choi, Jong-Hak (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Song, Hyun-Ouk (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Ryu, Jae-Sook (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine) ;
  • Ahn, Myoung-Hee (Department of Parasitology and Institute of Biomedical Science, Hanyang University College of Medicine)
  • 발행 : 2008.09.30
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초록

During Toxoplasma gondii infection, macrophages, dendritic cells, and neutrophils are important sources of pro-inflammatory cytokines from the host. To counteract the pro-inflammatory activities, T. gondii is known to have several mechanisms inducing down-regulation of the host immunity. In the present study, we analyzed the production of pro- and anti-inflammatory cytokines from a human myelomonocytic cell line, THP-1 cells, in response to treatment with T. gondii lysate or lipopolysaccharide (LPS). Treatment of THP-1 cells with LPS induced production of IL-12, TNF-$\alpha$, IL-8, and IL-10. Co-treatment of THP-1 cells with T. gondii lysate inhibited the LPS-induced IL-12, IL-8 and TNF-$\alpha$ expression, but increased the level of IL-10 synergistically. IL-12 and IL-10 production was down-regulated by anti-human toll-like receptor (TLR)-2 and TLR4 antibodies. T. gondii lysate triggered nuclear factor (NF)-${\kappa}B$-dependent IL-8 expression in HEK293 cells transfected with TLR2. It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2-mediated NF-${\kappa}B$ activation.

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