한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제25권4호
  • /
  • Pages.268-273
  • /
  • 2008
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

옻 추출물을 이용한 돈육의 생산과 가공기술의 개발 V. 옻 추출물 급여 후 돼지 림프구의 분포와 소장 점막 내부의 형태 변화

Production of High Quality Pork with Feed Additives of Lacquer Tree Extracts and Development of Meat Processing Technique V. Lymphocyte Distribution and Morphological Change in Small Intestine of Pigs after Fed with Fermented Lacquer Tree Extracts

  • 김상현 (한국생명공학연구원 국가영장류센터) ;
  • 성낙주 (경상대학교 자연과학대학) ;
  • 박중춘 (경상대학교 농업생명과학대학, 생명과학연구소) ;
  • 최충국 (경상대학교 농업생명과학대학, 생명과학연구소) ;
  • 송영민 (진주산업대학교 동물자원공학과) ;
  • 조규완 (경상대학교 수의과대학, 생명과학연구소, 동물과학연구소) ;
  • 차혜진 (경상대학교 수의과대학, 생명과학연구소, 동물과학연구소) ;
  • 김용환 (경상대학교 수의과대학, 생명과학연구소, 동물과학연구소)
  • Kim, Sang-Hyun (The National Primate Research Center, KRIBB) ;
  • Seuong, Nak-Ju (College of Natural Science, Gyeongsang National University) ;
  • Park, Joong-Choon (College of Agricultural & Life Science and Research Institute of Life Science, Gyeongsang National University) ;
  • Choi, Chung-Kuk (College of Agricultural & Life Science and Research Institute of Life Science, Gyeongsang National University) ;
  • Song, Young-Min (Department of Animal Resource & Biotechnology, Jinju National University) ;
  • Cho, Kyu-Woan (College of Veterinary Medicine, Research Institute of Life Science, Institute of animal Medicine, Gyeongsang National University) ;
  • Cha, Hye-Jin (College of Veterinary Medicine, Research Institute of Life Science, Institute of animal Medicine, Gyeongsang National University) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Research Institute of Life Science, Institute of animal Medicine, Gyeongsang National University)
  • 발행 : 2008.08.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

For evaluation of immune stimulation effect of lacquer tree extracts, lymphocytes were counted by labeling of FITC-conjugated monoclonal antibody in the pheripheral blood of pigs that fed with a fodder supplemented by lacquer tree extracts. Populations of MHC-II+, CD4+, and CD8+T lymphocytes were increased more than 2% level after 1 week feed supply of the lacquer tree extracts. The increase of those T cells reached at maximum level after 2 weeks in the tested group. B lympyocytes with surface IgM were increased 5% after 1 week feed supply of the lacquer tree extracts, and their numbers reached maximum after 2 weeks in the tested group. For the assessment of cytotoxicity of the lacquer tree extracts, morphological changes were examined on the epithelial cells of small intestine from pigs fed with a fodder supplemented by 0.1 % lacquer tree extracts for 6 weeks (the tested group). Thin-sectioned tissue of small intestine was fixed with glutaraldehyde, then coated with gold particles, and the specimen was examined under scanning electron microscope. The villi on the mucus membrane of jejunum and ileum from the tested pigswere enlarged on the tip and were linked each other.

키워드

참고문헌

  1. 김태욱, 한국의 수목(옻나무과), 교학사. 2002: 482-483
  2. 농림부 산림청 임업 연구원. 옻나무 urushiol과 Flavonoids의 간독성및 면역증진과 암세포 증식 및 혈관신생 억제제 개발. 농림부 기술 개발 사업보고서. 2001: 187
  3. 농촌 진흥청. 한국산 옻나무 수액의 알레르기 유도 물질의 특성에 관한 연구. 농촌 진흥청 시험 보고서. 1990: 33
  4. 나천수, 정남철, 오광인, 옻나무 침액 성분 중 urushiol의 암세포 증식 억제 균과 -in vitro 세포 독성효과. 한국 임학회지. 1988 87(2): 260-264
  5. 신동원, 김남일, 여인석, 한 권으로 읽는 동의보감 (옻편), 도서출판 들녘, 1999:956-957
  6. 이진만, 이상한, 권상호, 김준한, 이동성, 문철호, 김종국, 2000, 기능성건강식품 제조/실험, 도서출판 동국. 2000: 311-338
  7. 임계택, 심재환. 옻나무 에탄올 추출물의 쥐 뇌세포에 대한 항산화 효과. 한국식품 과학 회지 1997; 29(6): 1248-1252
  8. 임계택, 심재환. 옻나무 추출물의 생리활성에 이용에 대한 연구. 옻나무 추출물의 생리학적 기능. 한국식품 과학회지. 1999; 31(1): 238-242
  9. 정대교, 송홍근. 김훈. 한국산 옻나무 수액의 알레르기 유도물질의 특성에 관한 연구-옻나무 수액의 분취방법. 농사시험연구 논문집. 1991; 33: 675-679
  10. 조영식. 옻 추출물의 세포독성 및 자궁경부암 유발인자 E6과 E7의 작용에 미치는 효과. 한국식품 과학회지. 2000 32(6): 1389-1393
  11. 최덕진, 운태진, 김태홍. 옻에 의한 전신성 접촉 피부염의 전신광화학 요법, 대한 피부과학회지. 2002; 40(5): 483-487
  12. Cavdan F. Effect of Rhus coriaria(Anacardiaceae) on susperoxide seavenging and xanthin oxidase activity. J Enzyme Med Chem. 2003; 18(1): 59-62 https://doi.org/10.1080/1475636031000069273
  13. Choi C, Han, HJ, Kwon D, Kim J, Cheon DS, Min K, Cho WS, Chung HK, Jung T, Jung K, and Chae, C: Antimicrobial susceptibility of pathogenic Escherichia coliisolates from pig in Korea. J VET Sci 2002 64(1): 71-73 https://doi.org/10.1292/jvms.64.71
  14. Choi J, Yoon BJ, Han YN, Lee KT, Ha J, Jang HJ, Park HJ. Antirheumatoid arthritis effect of Rhus vernicifluaand of the active component, sulfuretin Planta Med. 2003; 69(10): 899-904 https://doi.org/10.1055/s-2003-45097
  15. Dunne HW and Lenan AD. Disease of Swine. Iowa state univ. 5ed. 1975: 571-554
  16. Hong DH, Han SB, Lee CW, Park SH, Jeon YJ, Kim MJ, Kwak SS, Kim HM Cytotoxicity of urushiol isolated Sap of Korean lacquer(Rhus vernicifera stokes). Arch Pham RES, 1999 22(6): 638-641 https://doi.org/10.1007/BF02975339
  17. Kim IT, Park YM, Shin KM, Ha J, Choi J, Choi J, Park HJ, Lee C. Anti-inflammatory and anti-nociceptive effects of the extract Kalopanax piturus, Pueraria thunbergiana and Rhus vernicifula. J Ethnopharmachol, 2004; 94(1): 168-173
  18. Lee JC, Kim J, Jang YS. Ethanol- eluted extract ofRhus vernicifluastroke inhibit growth and induces apoptosis in human lymphoma cell. J Biochem Mol, 2003; 36(4): 337-343 https://doi.org/10.5483/BMBRep.2003.36.4.337
  19. Lee JC, Lee KY, Kim J, Na CS, Jung NC, Chung GH, Jang YS. Extract from Rhus vernicifluastroke is capable of inhibiting the growth of human lymphoma cells. Food Chem Toxicol, 2004; 42(9): 1383-1388 https://doi.org/10.1016/j.fct.2004.03.012
  20. Lim KT. Hu C, Kitts DD. Antioxidant activity of Rhus vernicifluastroke ethanol extract. Eood Chem. Toxicol, 2001; 39(3): 229-237
  21. Lim KT, Lee SJ, Hae KS, Lim K. Effect ofisolated from Rhus vernicifluastroke TPA induced apoptosis and production cytokines in cultured mouse primary splenocytes. Toxicol Lerr, 2003; 145(3): 261-267 https://doi.org/10.1016/S0378-4274(03)00304-7
  22. Moxley RA. Edema disease. Vet Clin North Am Food Anim Pract. 2000 16: 175-185 https://doi.org/10.1016/S0749-0720(15)30142-0
  23. Oh SH, Haw CR, Lee MH. Clinical and immunological feature of systemic contact dermatitis ingestion of Rhus (Toxicodenaron). Contact dermatitis, 2003; 48(5): 251-264 https://doi.org/10.1034/j.1600-0536.2003.00103.x
  24. Tangpu, V. and Yadav, A.K. Antidiarrheal activity of Rhus japonica ripen fruit extract in a mice. Fitoterapia, 2004 75(1): 39-44 https://doi.org/10.1016/j.fitote.2003.08.015