Production of High Quality Pork with Feed Additives of Lacquer Tree Extracts and Development of Meat Processing Technique V. Lymphocyte Distribution and Morphological Change in Small Intestine of Pigs after Fed with Fermented Lacquer Tree Extracts

옻 추출물을 이용한 돈육의 생산과 가공기술의 개발 V. 옻 추출물 급여 후 돼지 림프구의 분포와 소장 점막 내부의 형태 변화

  • Kim, Sang-Hyun (The National Primate Research Center, KRIBB) ;
  • Seuong, Nak-Ju (College of Natural Science, Gyeongsang National University) ;
  • Park, Joong-Choon (College of Agricultural & Life Science and Research Institute of Life Science, Gyeongsang National University) ;
  • Choi, Chung-Kuk (College of Agricultural & Life Science and Research Institute of Life Science, Gyeongsang National University) ;
  • Song, Young-Min (Department of Animal Resource & Biotechnology, Jinju National University) ;
  • Cho, Kyu-Woan (College of Veterinary Medicine, Research Institute of Life Science, Institute of animal Medicine, Gyeongsang National University) ;
  • Cha, Hye-Jin (College of Veterinary Medicine, Research Institute of Life Science, Institute of animal Medicine, Gyeongsang National University) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Research Institute of Life Science, Institute of animal Medicine, Gyeongsang National University)
  • 김상현 (한국생명공학연구원 국가영장류센터) ;
  • 성낙주 (경상대학교 자연과학대학) ;
  • 박중춘 (경상대학교 농업생명과학대학, 생명과학연구소) ;
  • 최충국 (경상대학교 농업생명과학대학, 생명과학연구소) ;
  • 송영민 (진주산업대학교 동물자원공학과) ;
  • 조규완 (경상대학교 수의과대학, 생명과학연구소, 동물과학연구소) ;
  • 차혜진 (경상대학교 수의과대학, 생명과학연구소, 동물과학연구소) ;
  • 김용환 (경상대학교 수의과대학, 생명과학연구소, 동물과학연구소)
  • Published : 2008.08.31

Abstract

For evaluation of immune stimulation effect of lacquer tree extracts, lymphocytes were counted by labeling of FITC-conjugated monoclonal antibody in the pheripheral blood of pigs that fed with a fodder supplemented by lacquer tree extracts. Populations of MHC-II+, CD4+, and CD8+T lymphocytes were increased more than 2% level after 1 week feed supply of the lacquer tree extracts. The increase of those T cells reached at maximum level after 2 weeks in the tested group. B lympyocytes with surface IgM were increased 5% after 1 week feed supply of the lacquer tree extracts, and their numbers reached maximum after 2 weeks in the tested group. For the assessment of cytotoxicity of the lacquer tree extracts, morphological changes were examined on the epithelial cells of small intestine from pigs fed with a fodder supplemented by 0.1 % lacquer tree extracts for 6 weeks (the tested group). Thin-sectioned tissue of small intestine was fixed with glutaraldehyde, then coated with gold particles, and the specimen was examined under scanning electron microscope. The villi on the mucus membrane of jejunum and ileum from the tested pigswere enlarged on the tip and were linked each other.

Keywords

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